TOPIC 397 - FUNCTIONALLY CLOSED PURIFICATION AND ELUTION OF UNTOUCHED T CELLS USING CLEAVABLE MAGNETIC BEADS WITH RATCHETING CYTOMETRY

主题 397 - 使用可切割磁珠和棘轮细胞术对未接触的 T 细胞进行功能封闭纯化和洗脱

基本信息

  • 批准号:
    10287169
  • 负责人:
  • 金额:
    $ 39.8万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2020
  • 资助国家:
    美国
  • 起止时间:
    2020-09-08 至 2021-06-07
  • 项目状态:
    已结题

项目摘要

The goal of this effort is to develop a functionally closed, high throughput magnetic cell purification system which can achieve 1) purification of T cell populations directly from a leukopak biomatrix via positive selection and 2) cleavage of bead-cell bonds resulting in untouched T cells for cell therapy production. We believe that a functionally closed system that can achieve high purity positive selection of target cell populations will improve potency and repeatability of the final therapeutic cell batches as well as enable therapeutic production using more nuanced and delicate cell types. In Phase I we will perform separations using Ferrologix existing T cell purification cartridge and commercially available cleavable magnetic beads. Leukopak/apheresis specimens will be first tagged with anti-CD3 cleavable magnetic beads, separated with Ferrologix ratcheting cytoemtry cartridge, incubated within the cartridge with bead cleaving agents, and T cell elution off chip while the beads remain trapped in the cartridge. Ferrologix untouched T cell workflow will be compared in split sample fashion with two other cleavable bead workflows from Miltenyi and StemCell Technologies. Workflows will be compared on the basis of processing time, purity, viability, and successful cleavage of magnetic beads.
该努力的目标是开发功能封闭的高通量磁性细胞纯化系统,其可以实现1)通过阳性选择直接从leukopak生物基质纯化T细胞群和2)切割珠-细胞键,产生用于细胞治疗生产的未接触的T细胞。我们相信,可以实现靶细胞群的高纯度阳性选择的功能封闭系统将提高最终治疗性细胞批次的效力和可重复性,并能够使用更细微和微妙的细胞类型进行治疗性生产。在第一阶段,我们将使用Ferrologix现有的T细胞纯化柱和市售的可切割磁珠进行分离。首先用抗CD 3可切割磁珠标记Leukopak/单采标本,用Ferrologix棘轮细胞计数检测盒分离,在检测盒内用磁珠切割剂孵育,T细胞洗脱出芯片,同时磁珠仍截留在检测盒中。Ferrologix未接触T细胞工作流程将以分离样品的方式与Miltenyi和StemCell Technologies的其他两种可切割珠工作流程进行比较。将根据处理时间、纯度、活力和磁珠的成功切割来比较工作流程。

项目成果

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