Cochlear Vasculature and Its Role in Strial Atrophy

项目总结 感音神经性听力损失通常与血管纹中的血管系统退化有关， 耳蜗侧壁上皮带血管，是听力功能所必需的。工业生产的运行机制 然而，萎缩仍然难以捉摸，而且没有根治性或预防性的干预措施。这项研究测试了两个 对增强或“正常化”耳蜗血管功能的假说的预测可能会减缓或 血管纹的逆转萎缩：1)受影响小鼠的纹状萎缩与血管有关 血管纹的异常和血管完整性的丧失以及2)改善内皮屏障 功能、血管张力和血管生成保护小鼠免受氨基糖苷类药物诱导的血管萎缩。至 衡量这种变化，该方法使用创新的技术来比较，在正常和受伤的内心 EAR，包括整个耳蜗区微血管在内的血管的三维形态以及分子 在单细胞水平上的耳蜗血管的构成。因此，除了检验上述预测外， 这项研究可以确定细胞亚型、分子机制和结构，对 耳蜗内的循环和溶质运输，这对理解和管理具有广泛的意义 内耳疾病。 好了！

PROJECT SUMMARY Sensorineural hearing loss is often associated with degeneration of the vasculature in the stria vascularis, a vascularized epithelium in the lateral wall of the cochlea required for hearing function. The mechanism for strial atrophy remains elusive, however, and no curative or preventative interventions exist. This research tests two predictions of the hypothesis that augmenting or “normalizing” vascular function in the cochlea may slow or reverse atrophy in the stria vascularis: 1) that strial atrophy in affected mice is associated with vascular abnormalities and loss of vascular integrity in the stria vascularis and 2) that improved endothelial barrier function, vascular tone and angiogenesis protects mice against aminoglycoside-induced strial atrophy. To measure such changes, the approach uses innovative techniques to compare, in normal and injured inner ears, the morphology of vasculature including microvessels in the whole cochlea in 3D as well as the molecular makeup of the cochlear vasculature at the single cell level. Thus, in addition to testing the predictions above, this research could identify cell subtypes, molecular mechanisms and structures of potential significance to circulation and solute transport in the cochlea, which has broad implications for understanding and managing inner ear disease. !