Lens epithelial cell heterogeneity during development

发育过程中晶状体上皮细胞的异质性

• 批准号: 10311989
• 负责人: Xiaohua Gong
• 金额: $ 35.03万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-02-01 至 2024-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10311989
• 关键词: 1 year old; AQP1 gene; Age; Aging; Anterior; Antibodies; Candidate Disease Gene; Cataract; Cell physiology; Cells; Development; Disease; Epithelial; Epithelial Cells; Exhibits; Eye; Fiber; Gene Expression Profile; Gene Mutation; Genes; Genetic Transcription; Goals; Growth; Heterogeneity; Homeostasis; Image; Impairment; Knock-out; Knowledge; Lead; Lens Fiber; Lens development; Light; Literature; MAP2K1 gene; Maintenance; Mediating; Microphthalmos; Molecular; Mus; Outcome; Pathologic; Phase; Phenotype; Physiological; Property; Proteins; Regulation; Resolution; Retina; Shapes; Signal Pathway; Signal Transduction; Signal Transduction Pathway; Stimulus; Testing; Tissues; Transgenic Animals; Transgenic Mice; Up-Regulation; Water; age related; base; cell type; experimental study; high resolution imaging; insight; lens; monolayer; novel; postnatal; postnatal development; rapid growth; response; single cell analysis; single-cell RNA sequencing; transcription factor; transcriptomics

The proliferation, differentiation and homeostasis of lens anterior monolayer epithelium determine the lens growth, size, shape and transparency, which are absolutely required for focusing and transmitting a light image onto the retina. The goal of this proposal is to test a novel hypothesis that the intrinsic heterogeneity of lens epithelial cells regulates lens growth and homeostasis, and distinctive cell clusters of lens monolayer epithelium are precisely organized for mediating external signals of surrounding ocular tissues and environmental stimuli to regulate the lens growth, size, shape and transparency, and that disruptions of specific cell cluster(s) impair the regulation of lens growth and homeostasis to lead to various lens and/or eye pathological outcomes including cataracts, microphthalmia, and macrophthalmia.

晶状体前单层上皮的增殖、分化和稳态