Lipocalin 2 as a regulator of phospholipid metabolism in adipose mitochondrial bioenergetics

磷脂，特别是心磷脂(CL)和磷脂酸(PA)是线粒体膜结构和动力学的重要调节因子。内质网(ER)是线粒体磷脂生物合成的主要部位，内质网-线粒体接触部位或所谓的线粒体-内质网相关膜(MAM)是内质网与线粒体之间磷脂交换的平台。MAM功能障碍扰乱磷脂代谢，从而破坏线粒体的结构和功能。因此，维持MAM中磷脂代谢的动态平衡对于线粒体的正常结构和功能至关重要。然而，我们对脂肪细胞膜上磷脂的生物合成和交换是如何调控的，以及这些调控过程中涉及哪些因素，我们知之甚少。我们一直在研究Lipocalin 2(Lcn2)作为生热脂肪细胞激活和线粒体呼吸的关键调节因子的代谢作用。Lcn2KO小鼠表现出产热功能受损，并加剧了饮食诱导的肥胖和胰岛素抵抗。值得注意的是，Lcn2的缺失扰乱了棕色和米色脂肪细胞的线粒体结构，扰乱了线粒体的动力学，损害了线粒体的呼吸，而Lcn2在脂肪组织中的过表达促进了产热，增加了棕色脂肪组织的线粒体代谢，并在没有生热刺激的情况下激活了白色脂肪组织的褐化。最有趣的是，我们发现Lcn2定位于脂肪细胞的MAM，并且Lcn2具有强烈和选择性地结合磷脂酸(PA)的能力。我们推测，Lcn2作为一种新的PA结合蛋白，通过MAM调节PA的转运，为CL的生物合成提供前体，从而维持线粒体磷脂的动态平衡和健康。我们提出了三个目标：1)确定Lcn2在PA转运中的作用；2)确定Lcn2在CL生物合成中的作用；3)确定Lcn2在MAM功能和生热脂肪细胞线粒体动力学中的作用。我们相信，该项目将为了解肥胖和衰老过程中热源性脂肪细胞线粒体生物能量学失调的分子机制开辟一条新的途径。

Phospholipids, particularly cardiolipin (CL) and phosphatidic acid (PA) are known as critical regulators of mitochondrial membrane architecture and dynamics. Endoplasmic reticulum (ER) is the major site of phospholipid biosynthesis for mitochondria; ER-mitochondria contact sites or so-called Mitochondria-ER Associated Membranes (MAMs) serve as the platforms for phospholipid exchange between ER and mitochondria. MAM dysfunction disturbs phospholipid metabolism, thereby disrupting mitochondrial architecture and function. As such, maintaining metabolic homeostasis of phospholipids at MAMs is essential for the normal architecture and functioning of mitochondria. However, we have little or no knowledge on how phospholipid biosynthesis and exchange at the MAMs is regulated in adipocytes and what factors are involved in these regulatory processes. We have been characterizing the metabolic role of lipocalin 2 (Lcn2) as a critical regulator of thermogenic adipocyte activation and mitochondrial respiration. Lcn2 KO mice exhibit impaired thermogenesis as well as exacerbated diet-induced obesity and insulin resistance. Notably, deletion of Lcn2 disrupts mitochondrial architecture, dysregulates mitochondrial dynamics, and impairs mitochondrial respiration in brown and beige adipocytes, whereas overexpression of Lcn2 in adipose tissue promotes thermogenesis, increases mitochondrial metabolism of brown adipose tissue, and activates beiging of white adipose tissue without thermogenic stimulation. Most intriguingly, we discovered that Lcn2 is localized at MAMs in adipocytes, and Lcn2 has ability to strongly and selectively bind phosphatidic acid (PA). We hypothesize that Lcn2 plays a key role as a novel PA binding protein in the regulation of PA transport through MAM providing the precursor for CL biosynthesis, thereby maintaining mitochondrial phospholipid homeostasis and health. We proposed three Aims to 1) characterize the role of Lcn2 in PA transport, 2) determine the role of Lcn2 in CL biosynthesis, and 3) determine the role of Lcn2 in MAM function and mitochondrial dynamics in thermogenic adipocytes. We believe that this project will open a novel avenue to understand the molecular mechanisms for the dysregulation of mitochondrial bioenergetics in thermogenic adipocytes in obesity and during aging.