Core B: Liver Metastasis Resource Core (LMRC)

核心 B：肝转移资源核心 (LMRC)

• 批准号: 10331762
• 负责人: Edwin Melencio Posadas
• 金额: $ 24.64万
• 依托单位: CEDARS-SINAI MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-01-21 至 2024-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10331762
• 关键词: Address; Animal Model; Autopsy; Basic Science; Biological; Biological Assay; Biological Markers; Biomedical Research; Biostatistics Core; Blood; Blood specimen; Cancer Etiology; Cessation of life; Clinic; Clinical; Clinical Sciences; Collection; Colonic Neoplasms; Complement; Cost efficiency; Data Analytics; Defense Mechanisms; Development; Engraftment; FDA approved; Face; Generations; Goals; Growth; Health Personnel; Healthcare Systems; Human; Institutional Review Boards; Investigation; Liver; Los Angeles; Malignant Neoplasms; Medical center; Metastatic Neoplasm to the Liver; Methods; Modeling; Mus; NanoVelcro; Natural History; Nebraska; Neoplasm Circulating Cells; Neoplasm Metastasis; Output; Pathologic; Patients; Persons; Pharmacology; Physiological; Procedures; Process; Productivity; Program Efficiency; Prostate; Prostatic Neoplasms; Protocols documentation; Research; Research Personnel; Resources; Sampling; Services; Signal Transduction; Site; Specimen; Standardization; Tissue Banks; Tissue Model; Tissues; Transgenic Organisms; Translating; Tumor Tissue; United States; Universities; Variant; Work; analytical tool; blood-based biomarker; data communication; data sharing; experience; extracellular vesicles; human tissue; in vivo Model; innovative technologies; insight; liver development; model development; mouse model; nano-string; nanotechnology platform; neoplastic cell; new technology; novel; operation; pancreatic cancer model; pancreatic neoplasm; patient derived xenograft model; programs; synergism; tool; tumor; tumor microenvironment

ABSTRACT Core B [The Liver Metastasis Resource Core (LMRC)] will supply human tissues and animal models to support the four Projects in studying interactions between the liver microenvironment and the metastatic tumor cells that colonize it. The Core will standardize operations across Projects through three functions: (1) Providing human tumor tissue and blood specimens, along with thorough pathological annotation; (2) Producing novel transgenic and orthotopic mouse models of liver metastasis and patient-derived xenografts (PDX) in mice using human metastatic tumor specimens; and (3) Analyzing mouse and human biomarkers, as well as isolating and characterizing circulating tumor cells (CTCs) and extracellular vesicles (EVs) using several approaches, including a suite of NanoVelcro Assays, an emerging nanotechnology platform. Core B will provide these resources under the aegis of well-coordinated and efficient central processes that maximize productivity and cost efficiency for the Program. The Core has the following specific aims: AIM 1. Procure and process human biospecimens, for appropriate distribution to investigators. AIM 2. Generate transgenic, orthotopic, and PDX mouse models of liver metastasis. AIM 3. Collect and characterize blood components, including CTCs and EVs. In summary, Core B will provide investigators with well-annotated tissue specimens, managed under harmonized operating procedures across all collection sites and Projects. The Core will house all resources and expertise, professional and technical, to generate physiologically relevant mouse models to support the Projects. Centralized pathologic, clinical and technical services will enhance communication of data and analytical insight across all Projects, supporting the Program's overall goals.

摘要 核心B [肝转移资源核心（LMRC）]将提供人体组织和动物模型，以支持 研究肝脏微环境与转移性肿瘤细胞之间相互作用的四个项目， 核心将通过三个功能标准化跨项目的操作：（1）提供人力资源， 肿瘤组织和血液标本，沿着进行彻底的病理学注释;（2）产生新的转基因 和原位小鼠肝转移模型和患者来源的异种移植物（PDX）， 人转移性肿瘤样本;和（3）分析小鼠和人生物标志物，以及分离 以及使用几种方法表征循环肿瘤细胞（CTC）和细胞外囊泡（EV）， 包括一套NanoVelcro Assays，一种新兴的纳米技术平台。核心B将提供这些 在协调良好且高效的中央流程的支持下，资源可以最大限度地提高生产力， 方案的成本效益。 该核心有以下具体目标： AIM 1.采集和处理人体生物标本，以便适当分发给研究者。 AIM 2.生成肝转移的转基因、原位和PDX小鼠模型。 AIM 3.收集和表征血液成分，包括CTC和EV。 总之，核心B将为研究者提供注释良好的组织标本，按照 统一了所有采集点和项目的操作程序。核心将容纳所有资源， 该研究所拥有专业和技术知识，以生成生理学相关的小鼠模型，以支持项目。 集中的病理、临床和技术服务将加强数据和分析见解的交流 在所有项目中，支持计划的总体目标。