Development of Wireless Equipment for Autonomous Rodent Infusion Tasks

项目总结 尽管在临床前和临床成瘾领域都取得了重大进展，但美国公民的数量 物质使用障碍(Suds)和致命性过量用药的结果在过去不断增加。 二十年了。为了解决这一令人担忧的健康问题，需要翻译的啮齿动物模型来揭示神经 电路和基础的细胞和分子机制的发展和维持的SUDS。在……里面 在这方面，静脉注射自我给药程序是啮齿动物SUD模型的黄金标准。然而， 尽管这是最可靠的程序之一，具有明显的表面有效性，但该程序仍受到其 系留性质，将其使用限制在啮齿动物暴露于未富集物的有限空间 食物、水或社会互动有限或无法获得的环境。环境或环境是至关重要的 SUD的组件。在啮齿动物模型中，有证据表明，自愿社交和 非社会奖励，如跑轮子，以及操作者送来的美味食物会减少 消费、升级和恢复药物自我管理。在这里，我们建议使用我们的 在无线技术、设备开发和药物滥用责任的啮齿动物模型方面的互补专业知识 建立无线控制的静脉注射自我给药实验，在复杂和更具行为学的- 相关环境，如丰富的实验室住宅。虽然无线给药设备具有 以前开发的，它们只允许小容量和有限数量的受控输液。 静脉注射自我给药程序通常由长期的每日一次组成，在此期间 为了评估药物滥用的责任，需要多次受控的药物摄入量，因此需要更大的药物数量。至 解决这一限制，并允许在丰富的实验室家庭笼子中评估药物滥用责任 提出1)开发一种无线控制的可穿戴式静脉留置导管药库 2)使用蓝牙网格技术实现基于家庭的静脉自我给药程序 使用交互式互联设备。我们设想，这些新的开源方法将扩大 通过支持新的实验设计来改善现有的临床前SUD模型的翻译价值 旨在开发药物使用障碍治疗的战略。

PROJECT SUMMARY Despite major advances in both the preclinical and clinical addiction fields, the number of US citizens afflicted by substance use disorders (SUDs) and the lethal overdose outcomes has continuously increased over the past two decades. To tackle this alarming health issue, translational rodent models are needed to uncover the neural circuits and the cellular and molecular mechanisms underlying the development and maintenance of SUDs. In this regard, intravenous self-administration procedures is the gold standard for rodent SUD models. However, despite being one of the most reliable procedures, with clear face validity, this procedure is still limited by its tethered nature, constraining its use to restricted spaces in which rodents are exposed to unenriched environments with limited or no access food, water, or social interaction. The setting or environment is a critical component of SUD. In rodent models, evidence has shown that availability for volitional social interaction and non-social rewards such as wheel running, and operant-delivered palatable foods can decrease the consumption, the escalation, and the reinstatement in drug self-administration. Here we propose to use our complementary expertise in wireless technology, device development, and rodent models of drug abuse liability to establish wirelessly controlled intravenous self-administration experiments in complex and more ethologically- relevant environments such as enriched laboratory homecages. While wireless drug administration devices have previously been developed, they only allow for small volume and restricted number of controlled infusions. Intravenous self-administration procedures are generally composed of long-term daily session during which multiple controlled drug intakes and thus larger drug volumes are necessary to assess drug abuse liability. To tackle this limitation and permit the assessment of drug abuse liability in enriched laboratory home cages we propose to 1) develop a wireless-controlled wearable drug reservoir connected to intravenous indwelling catheter and 2) use Bluetooth mesh technology to enable homecage-based intravenous self-administration procedures using interactive connected devices. We envision that these new open-source approaches will broaden the translational value of pre-clinical SUD models by enabling new experimental designs to improve current strategies aiming at developing substance use disorders treatments.