The Role of Ventromedial Hypothalamus Cholecystokinin Receptor B Containing Neurons in Regulation of Hypoglycemia and Hypoglycemia-Associated Autonomic Failure

摘要 我的F32题为“腹内侧下丘脑胆囊收缩素B受体的作用， 神经元（VMNCCKBR）在调节脑功能减退和脑功能减退相关的自主神经功能衰竭”需要 广泛使用遗传小鼠模型和神经外科技术来评估特定神经元在 葡萄糖代谢的调节。2020年3月20日，密歇根大学的所有研究都停止了， 是为了减少70%以上的鼠群由于动物设施人员的减少和有限的 PPE，我们仅限于维持水平养殖，直至二零二零年六月中旬。 我的实验要求我在小鼠8 - 12周龄时进行神经外科手术， 手术后4 - 8周的动物，因此已经指定用于实验的几组小鼠， 2020年3月下旬至6月，由于繁殖新小鼠所需的时间， 实验是允许的，神经外科的年龄要求，我无法重新开始手术，直到 2020年10月，直到2020年12月才能开始代谢实验。因此，由于其特殊性， 我的训练和实验类型，我执行，我失去了额外的6个月的实验训练 除了由于实验室关闭而损失的3个月之外。 在这次延期所要求的额外6个月期间，我将特别专注于延长我的 使用光遗传学评估VMNCCKBR神经元的生理学并开发额外的病毒追踪的培训 评估VMN神经元连接的策略。申请的资金将提供我的津贴和机构 在我接受培训的同时，

Abstract My F32 entitled “The Role of Ventromedial Hypothalamus Cholecystokinin B Receptor Containing Neurons (VMNCCKBR) in Regulation of Hypoglycemia and Hypoglycemia Associated Autonomic Failure” requires extensive use of genetic mouse models and neurosurgical techniques to evaluate the role of specific neurons in the regulation of glucose metabolism. On March 20, 2020, all research at University of Michigan stopped and I was required to reduce my mouse colony by over 70%. Due to reductions in animal facility staffing and limited PPE, we were restricted to maintenance level breeding until mid-June 2020. My experiments require that I perform neurosurgery when the mice are 8 – 12 weeks of age and study the animals 4 – 8 weeks after surgery, thus several cohorts of mice that had been designated for experiments in late March through June 2020 were lost. Due to the time required to generate new mice once breeding for experiments was allowed and the age requirements for neurosurgery, I was unable to restart surgeries until October 2020 and could not start metabolic experiments until December 2020. Thus, due to the specific nature of my training and the type of experiments that I perform, I lost an additional 6 months of experimental training in addition to the 3 months that were lost due to the lab shut down. During the additional 6 months requested in this extension, I will specifically focus on extending my training in using optogenetics to assess the physiology of VMNCCKBR neurons and develop additional viral tracing strategies to evaluate VMN neuron connectivity. The funds requested will provide my stipend and institutional support while I receive this training.