Integrative analyses of the kinetochore and the spindle assembly checkpoint

动粒和纺锤体装配检查点的综合分析

• 批准号: 10393295
• 负责人: Ajit Joglekar
• 金额: $ 5.97万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-07-01 至 2023-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10393295
• 关键词: Academia; Award; Basic Science; Biochemical; Biophysics; Cancerous; Cell Cycle Regulation; Cell division; Cells; Cellular biology; Chromosomes; Code; Complex; Data Analyses; Dedications; Development; Ensure; Faculty; Financial Support; Funding; Future; Genome; Institution; Kinetochores; Learning; Mentorship; Methods; Microscopy; Mitosis; Molecular; Negative Staining; Parents; Phase; Polishes; Process; Protein Engineering; Proteins; Pythons; Research; Research Project Grants; Resource Development; Schools; Science; Scientist; System; Talents; Time; Universities; Work; base; career; daughter cell; experience; graduate student; insight; meetings; molecular imaging; pleasure; programs; recruit; single molecule

Abstract My lab combines cell biology and biophysics to conduct integrative analyses of two complex cellular systems: the kinetochore, a protein machine that moves chromosomes during mitosis, and the Spindle Assembly Checkpoint (SAC), which is a cell cycle control. Even as we are making excellent progress on the stated aims of our project, I have started developing the next phase of this research that involves replacing components of the kinetochore protein machine with de novo designed proteins. For this exciting work, I recruited Mr. Martin Fernandez, a recent graduate from the Lehigh University. Martin works in my lab as a research technician, spending 60% of his time on active research involving benchwork, data analysis, and presentation, and the remaining 40% of the time on lab management duties. I have had the pleasure of working with Martin for over 6 months, and this experience makes it clear to me that: (a) Martin possesses the talent and dedication to emerge as a professional in the biomedical sciences, and (b) Martin has a passion for basic research and wants to ultimately join the academia. Based on my experience, I am confident that he will successfully apply for joining a graduate program in the coming year. Therefore, I want to maximize the impact of Martin’s work in my lab over his career by ensuring that: (a) he dedicates 100% of his time to research, and (b) he gets the full benefit of the mentorship and development resources that I can provide. For this reason, I, together with Martin, am applying for a diversity supplement to cover Martin’s efforts for 1 year. During this year, Martin will work essentially as a new graduate student in my lab and drive an exciting research project forward. He will learn cutting-edge experimental methods (negative-stain EM microscopy, single molecule imaging, etc.). He will also engage in professional development activities (learning to code using Python, networking with graduate students and faculty at my institution, presenting his work at the ASCB meeting, putting together a polished application for graduate school, etc.). I am confident that Martin will fully utilize the financial support and recognition that comes with this funding and start his career as a scientist in the near future.

摘要 我的实验室结合了细胞生物学和生物物理学，对两个复杂的 细胞系统：动粒，一种在有丝分裂期间移动染色体的蛋白质机器， 以及主轴组件检查点（SAC），其是细胞周期控制。即使我们正在 在我们项目的既定目标方面取得了出色的进展，我已经开始开发下一阶段的 这项研究涉及用从头开始的蛋白质替换动粒蛋白机器的组件， 设计蛋白质为了这项激动人心的工作，我聘请了马丁费尔南德斯先生，一位刚毕业的学生 来自利哈伊大学。马丁在我的实验室里做研究技术员， 时间在积极的研究，涉及工作台，数据分析和演示，其余的 40%的时间在实验室管理上。我有幸与马丁合作， 超过6个月，这段经历让我清楚地认识到：（a）马丁拥有天赋， 致力于成为生物医学科学的专业人士，（B）马丁有一种激情 进行基础研究，并希望最终加入学术界。根据我的经验，我 相信他将成功地申请加入研究生课程在未来一年。 因此，我想通过确保马丁在我的实验室的工作对他的职业生涯产生最大的影响， （a）他把100%的时间都花在研究上，（B）他得到了研究的全部好处。 我能提供的指导和发展资源。因此，我和马丁， 我正在申请一个多元化的补充，以涵盖马丁的努力为1年。今年，马丁 我将主要作为一名新的研究生在我的实验室工作，并推动一个令人兴奋的研究项目 性新他将学习尖端的实验方法（负染色EM显微镜，单 分子成像等）。他还将参与专业发展活动（学习 使用Python编写代码，与我所在机构的研究生和教师建立联系，介绍他的 在ASCB会议上工作，整理一份精美的研究生院申请表等）。我是 我相信马丁将充分利用随之而来的财政支持和认可， 在不久的将来开始他的科学家生涯。