Tissue Procurement and Processing Core

组织采购和加工核心

• 批准号: 10392979
• 负责人: Maki Nakayama
• 金额: $ 18.06万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-04-01 至 2025-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10392979
• 关键词: Adipocytes; Agreement; Alberta province; Antibodies; Antigens; Basic Science; Blood specimen; Cell Line; Cells; Clinical; Collaborations; Colorado; Communities; Complement; Consultations; Diabetes Mellitus; Disease model; Drug or chemical Tissue Distribution; Equipment; Formalin; Freezing; Funding; Generations; Histologic; Histology; Histopathology; Human; Immune; Individual; Institutional Review Boards; Insulin-Dependent Diabetes Mellitus; International; Letters; Maintenance; Mission; Non-Insulin-Dependent Diabetes Mellitus; Operative Surgical Procedures; Organoids; Pancreas; Paraffin Embedding; Paraffin Tissue; Pathogenesis; Pathology; Patients; Preparation; Procedures; Protocols documentation; Reagent; Research; Research Personnel; Resources; Rodent; Sampling; Services; Source; Spleen; Stains; Surveys; Thymus Gland; Tissue Embedding; Tissue Procurements; Tissue Sample; Tissues; Training; Universities; Work; base; biobank; cell repository; human pluripotent stem cell; human tissue; innovation; instrument; islet; liquid crystal polymer; material transfer agreement; member; tissue preparation; tissue processing

TISSUE PROCUREMENT AND PROCESSING CORE PROJECT SUMMARY/ABSTRACT The overall mission of the Tissue Procurement and Processing (TPP) Core is to facilitate access and usage of tissues and services to meet the specialized needs of the diabetes research community to expedite and catalyze innovative diabetes research at UC AMC. This core will establish and expand resources to assist DRC investigators with the acquisition and processing of rodent and human tissue, and specialized cell lines that are broadly required for diabetes research. For the past 20 years, the services provided by the BDC-based facility has provided high quality tissues to BDC investigators. DRC funding would allow us to expand access to all DRC investigators studying the pathogenesis of Type1 (T1D) and Type 2 (T2D) diabetes. In addition, there is increased need for primary human tissue. We have established a centralized facility to facilitate access to human tissues from external sources (University of Alberta, Edmonton; see letter of support), and internally through the UC-AMC Pathology Biorepository/Tissue Distribution Facility (see letter of support). Furthermore, administrative support for the preparation of IRB approval, human exempt protocols and material transfer agreements for these tissues will be available upon request by DRC members. The core will also provide diabetes-related histopathology services, training in basic and specialized sample preparation and processing procedures, and will offer DRC members access to resources such as validated commonly used reagents and equipment. Finally, the TPP Core will provide a cell banking service to distribute validated diabetes-related cell lines to DRC investigators. These services will facilitate individual studies, in addition to promoting collaborations between investigators. Major services to be provided by the TPP core include 1) tissue preparation, including rodent and human islet isolation and processing; 2) procurement of primary human tissues from local, national and international sources; 3) sample processing for histological analysis; 4) validated and optimized reagents, such as antibodies and probes; and 5) validated cell line distribution. These services have been selected based on a campus-wide survey that solicited information from UC AMC diabetes investigators about their research needs. These services have been selected based on a campus-wide survey that solicited information from UC AMC diabetes investigators about their research needs. More than 65% of our DRC membership, which represents >95% of our non-clinical research base indicated their need for tissue procurement and/or processing assistance.

组织采购和处理核心 项目总结/摘要 组织采购和处理（TPP）核心的总体使命是促进获取和使用 组织和服务，以满足糖尿病研究界的专门需求，以加快和催化 加州大学AMC分校的创新糖尿病研究。这一核心将建立和扩大资源，以协助刚果民主共和国 研究人员获得和处理啮齿动物和人类组织，以及专门的细胞系， 广泛用于糖尿病研究。在过去的20年里，由位于BDC的设施提供的服务 为BDC的调查人员提供了高质量的组织。刚果民主共和国的资金将使我们能够扩大对所有刚果民主共和国的访问 研究1型（T1 D）和2型（T2 D）糖尿病发病机制的研究人员。此外还有 增加了对原始人体组织的需求。我们建立了一个中央设施， 组织从外部来源（阿尔伯塔大学，埃德蒙顿;见支持信），并通过内部 UC-AMC病理学生物储存库/组织分销机构（见支持函）。此外，行政 支持IRB批准、人类豁免协议和材料转移协议的准备， 卫生纸将应要求提供给DRC成员。核心还将提供糖尿病相关的 组织病理学服务，基本和专业样本制备和处理程序的培训，以及 将为DRC成员提供资源，如经验证的常用试剂和设备。最后， TPP核心将提供细胞库服务，向刚果民主共和国分发经验证的糖尿病相关细胞系 investigators.这些服务将促进个人研究，并促进 investigators. TPP核心将提供的主要服务包括：1）组织制备，包括啮齿动物和 人胰岛分离和处理; 2）从地方、国家和国际组织采购原代人组织， 国际来源; 3）用于组织学分析的样品处理; 4）经验证和优化的试剂，例如 作为抗体和探针;和5）验证的细胞系分布。选择这些服务的依据是 一项全校范围的调查，从UC AMC糖尿病研究者那里征集有关他们研究需求的信息。 这些服务是根据校园范围内的调查选择的，该调查向加州大学AMC征求信息 糖尿病研究者了解他们的研究需求。超过65%的刚果民主共和国成员， 我们超过95%的非临床研究基地表示需要组织采购和/或处理协助。