Cell Imaging and Histology

细胞成像和组织学

• 批准号: 10400708
• 负责人: BROOKE M EMERLING
• 金额: $ 20.66万
• 依托单位: SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-05-01 至 2025-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10400708
• 关键词: AGTR2 gene; Algorithmic Analysis; Calendar; Cancer Center; Cancer Center Support Grant; Cells; Communication; Computer Workstations; Computer software; Contract Services; Core Facility; Cryoultramicrotomy; Data; Data Analyses; Doctor of Philosophy; Documentation; Employee; Equipment; Experimental Designs; Faculty; Feedback; Fluorescence; Fluorescence Resonance Energy Transfer; Grant; Histologic; Histology; Hybrids; Image; Image Analysis; Imaging technology; Immunofluorescence Immunologic; Immunohistochemistry; In Situ Hybridization; Institutional Review Boards; Label; Laboratories; Lasers; Leadership; Light; Maintenance; Malignant Neoplasms; Microscope; Microscopy; Mission; Pathology; Preparation; Publications; Research; Research Personnel; Reservations; Resolution; Resource Sharing; Scanning; Science; Scientist; Services; Slide; Stains; Surveys; System; Technology; Time; Tissues; Training; Transcript; Work; cellular imaging; cost effectiveness; fluorescence microscope; human tissue; imaging facilities; instrument; instrumentation; intravital microscopy; live cell imaging; meetings; member; programs; reconstruction; repaired

ABSTRACT – CELL IMAGING AND HISTOLOGY SHARED RESOURCE The mission of the Cell Imaging and Histology Shared Resource, which is managed by the Cancer Center, is to provide state-of-the-art imaging and histological services to Center researchers. The Core offers the instrumentation, expertise, training, and service to enable advanced cellular imaging and data analysis. The Cell Imaging facility's technology includes a recently acquired hybrid confocal/super-resolution Nikon A1 microscope system equipped for live-cell imaging, as well as three other confocal microscope systems—a Zeiss LSM 710 NLO laser point scanning system equipped with a multiphoton laser, An Olympus FloView 1000, and a Yokogawa spinning disk confocal microscope. In addition, the facility has six other fluorescence microscopes supporting a wide range of imaging technologies such as FRET, TIRF, live cell and time-lapse imaging. Most of the advanced microscopes at SBP are located within the facility. The facility's computer workstations provide access to image analysis software packages including Image-Pro plus, MetaMorph, Volocity, and Imaris 8.0, as well as Nikon software for off-line reconstruction of Nikon Super-Resolution data. The Histology facility services include preparation and cryosectioning of tissues; automated staining for H&E (Leica XLf), and immunohistochemistry, immunofluorescence, and in situ hybridization (Leica Bond RX). The facility's Aperio slide scanners (AT2 and FL ScanScopes, Leica) provide automated high-resolution scanning of whole IHC or fluorescence stained slides for data documentation, analysis, storage, and dissemination. The facility also provides assistance in obtaining human tissues, including assistance with IRB and MTA issues. Both the adjacent Cell Imaging and Histology facilities provide user training or full-service. Trained microscope users have 24/7 access to the equipment, with reservations for each instrument made through an on-line iLab calendar. The Core is very broadly used—in the past 5 years, the Core has contributed to research in 47 Cancer Center member laboratories representing all three Center programs and assisted in projects resulting in more than 190 cancer-relevant publications.

摘要-细胞成像和组织学共享资源 由癌症中心管理的细胞成像和组织学共享资源的使命是 为中心研究人员提供最先进的影像和组织学服务。酷睿提供了 仪器、专业知识、培训和服务，以实现先进的细胞成像和数据分析。《细胞》 成像设备的技术包括最近获得的混合共焦/超分辨率尼康A1显微镜 配备活细胞成像的系统，以及其他三个共焦显微镜系统-蔡司LSM 710 配备多光子激光器、奥林巴斯FloView 1000和横河的NLO激光点扫描系统 旋转圆盘共聚焦显微镜。此外，该设施还有另外六个荧光显微镜，支持 广泛的成像技术，如FRET、TIRF、活细胞和延时成像。大多数高级 SBP的显微镜位于设施内。该设施的计算机工作站提供对图像的访问 分析软件包包括Image-Pro plus、Metamorph、Volocity和Imaris 8.0，以及尼康 用于离线重建尼康超分辨率数据的软件。组织学设施服务包括 组织的制备和冷冻切片；H&E(徕卡XLF)的自动染色，以及免疫组织化学， 免疫荧光和原位杂交(徕卡Bond RX)。该设施的Aperio幻灯片扫描仪(AT2和FL ScanScope，徕卡)提供对整个IHC或荧光染色幻灯片的自动高分辨率扫描 数据记录、分析、存储和传播。该设施还提供帮助，以获得 人体组织，包括协助处理IRB和MTA问题。相邻细胞影像与组织学 设施提供用户培训或全方位服务。训练有素的显微镜用户可以全天候使用设备， 通过在线iLab日历预订每台仪器。核心应用非常广泛--在 在过去的5年里，核心为代表所有癌症中心成员的47个实验室的研究做出了贡献 三个中心项目，并协助项目，出版了190多种癌症相关出版物。