Association of CBP+ Streptococcus mutans with root caries in older adults

CBP 变形链球菌与老年人根龋的关联

• 批准号: 10401604
• 负责人: Jacqueline Abranches
• 金额: $ 11.09万
• 依托单位: UNIVERSITY OF FLORIDA
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-05-17 至 2022-07-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10401604
• 关键词: Aftercare; Age-Years; Bacteria; Binding; Binding Proteins; Biochemical Genetics; Caries prevention; Child; Clinical; Collagen; Cross-Sectional Studies; Dental Schools; Dental caries; Dentin; Dentists; Development; Elderly; Epithelial Cells; Etiology; Gingival Recession; Goals; Infection; Infective endocarditis; Intervention; Lead; Lesion; Life Expectancy; Mediating; Membrane Proteins; Mentors; Modeling; Names; Oral; Plant Roots; Population; Rattus; Recurrence; Research; Risk; Root Caries; Scientist; Severities; Streptococcus mutans; Surface; Tooth structure; Training; Translational Research; Work; career; cognitive ability; demineralization; dental biofilm; early childhood; experience; genetic approach; microbial signature; new product development; next generation; older patient; oral cavity epithelium; oral infection; oral pathogen; pathogen; prevent; skills; socioeconomics

Due to the prolonged life expectancy and retention of teeth combined with socioeconomic limitations, physical and cognitive abilities, older patients are highly vulnerable to root caries. In the U.S, 64% of adults older than 60 years of age experience root caries. The oral pathogen Streptococcus mutans is a keystone pathogen in dental caries due to its direct association with caries initiation and development. Nearly 20% of S. mutans strains express collagen-binding proteins (CBPs), specifically Cnm and Cbm, that mediate binding to collagen- rich surfaces. Our group showed that Cnm also mediates invasion of oral epithelial cells and avid binding to dentin and root surfaces (both are rich in collagen). Using a rat caries model, we showed that Cnm contributes to oral colonization, maturation of the dental biofilm, and severity of coronal caries. Moreover, in a recent cross-sectional study with children, we observed a strong association between infection with S. mutans CBP+ strains and early childhood caries (ECC) recurrence. In cases of gingival recession, the dentin is often exposed due to physical abrasion and demineralization. As collagen is the main organic material of dentin and, considering our recent observation that CBP+ S. mutans strains are strongly associated with ECC recurrence, we hypothesize that older adults experiencing gingival recession who are also infected with CBP+ S. mutans are at greater risk of developing root caries than those infected with CBP– S. mutans strains. However, clinical evidence correlating the presence of CBP+ S. mutans strains with root caries to support this hypothesis is lacking. The goal of this supplement application is to identify microbial signatures associated with root caries in older adult populations and determine whether infection with CBP+ S. mutans strains is associated with increased risk of root caries. Thus, findings from this supplement application can lead to a paradigm shift from clinical interventions focused solely on treatment after the lesion appears to root caries prevention and treatment by integration of this information into the assessment of caries risk. Dr. Garcia's career goal is to become an independent clinician-scientist at a research-intensive dental school conducting clinical- translational research, and serving as a mentor to the next generation clinician scientists. This supplement will allow her to obtain the additional training (courses) and experience (new lab skills) to make a smooth transition into an independent career as a dentist-scientist. Additionally, an outstanding group of collegial mentors comprised of microbiologists and a dentist-scientist will work closely with the candidate to provide guidance in professional development and with the scientific aspect of this proposal.

由于预期寿命延长和牙齿保留以及社会经济限制， 和认知能力，老年患者非常容易患根龋。在美国，64%的老年人 60多岁的人有过根面龋。口腔病原体变形链球菌是口腔疾病的关键病原体 龋齿，因为它与龋齿的发生和发展直接相关。近20%的S。变形 菌株表达胶原结合蛋白（CBP），特别是Cnm和Cbm，其介导与胶原的结合。 丰富的表面。我们的研究小组表明，Cnm还介导口腔上皮细胞的侵袭，并与 牙本质和牙根表面（两者都富含胶原蛋白）。使用大鼠龋齿模型，我们表明Cnm有助于 口腔定植、牙齿生物膜的成熟和冠状龋的严重程度。此外，在最近的 在儿童的横断面研究中，我们观察到感染S.变异CBP+ 菌株和早期儿童龋齿（ECC）复发。在牙龈萎缩的情况下，牙本质往往是暴露 由于物理磨损和脱矿作用。由于胶原蛋白是牙本质的主要有机物质， 考虑到我们最近的观察，CBP+ S.变异株与ECC复发密切相关， 我们假设，老年人经历牙龈萎缩谁也感染了CBP+ S。变形 比那些感染了CBP- S的人患牙根龋的风险更大。变异株但临床 与CBP+ S存在相关的证据。支持这一假设的变异株与根龋 缺乏本补充申请的目的是确定与根龋相关的微生物特征， 老年人群，并确定是否感染CBP+ S。变异株与 增加根龋的风险。因此，这项补充应用的发现可能会导致从 临床干预只集中在病变出现后的治疗，以预防根龋， 通过将这些信息整合到龋齿风险评估中进行治疗。加西亚医生的职业目标是 成为一名独立的临床医生，科学家在研究密集型牙科学校进行临床- 转化研究，并作为下一代临床科学家的导师。该补充将 让她获得额外的培训（课程）和经验（新的实验室技能），以顺利过渡 成为一名独立的牙医科学家此外，一群杰出的大学导师 由微生物学家和牙医科学家组成，将与候选人密切合作，提供指导， 专业发展和科学方面的这一建议。