Intensification of metallo-enzyme production to unlock sustainable biocatalytic hydrogenation

强化金属酶生产以实现可持续的生物催化氢化

• 批准号: 10065700
• 金额: $ 9.08万
• 依托单位: HYDREGEN LIMITED
• 依托单位国家: 英国
• 项目类别: Collaborative R&D
• 财政年份: 2023
• 资助国家: 英国
• 起止时间: 2023 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=10065700
• 关键词: Intensification; metallo; enzyme; production; unlock

**HydRegen** is a 2021 spin-out from the University of Oxford's Department of Chemistry.**Our vision:** The HydRegen technologies offer the potential for cleaner, safer, faster and cheaper chemical manufacture.Deep understanding of biology and chemistry allow the HydRegen team to select the best components from biology and use them to develop robust biotechnologies that solve real problems in the chemicals sectors.We expect our technologies to play a part in the UK chemicals sector meeting ambitious Net Zero emissions targets (by 2050) and increasing adoption of enabling technologies such as Industrial Biocatalysis and continuous flow process.Currently we are addressing challenges in the manufacture of pharmaceuticals and speciality chemicals (e.g. flavours and fragrances) and exploring the potential of our technologies for larger-scale, lower-value chemical production.**Dr Morra's Group (University of Nottingham)** have expertise in novel hydrogenase enzymes that are able to cycle H2 and H+. Theses enzymes are of interest to HydRegen due to their unusual balance in 'ease of production' and 'ease of handling'. Dr Morra's team have know-how and facilities in enzyme production spanning early-stage academic research through to evaluation of scalable enzyme production in bioreactors (up to 100L).**Project focus:** here we tackle challenges in manufacturing readiness of the HydRegen technologies by intensifying and scaling enzyme production, and applying the enzyme generated to a demonstrator process for quinuclidinol production. Quinuclidinol is an important chemical building-block present in a number of active pharmaceuticals.Dr Morra and HydRegen started their collaboration in 2022 via BBSRC-NIBB funding. This project allows both partners to realise the potential of this collaboration.**Project outputs:*** Lower cost-of-production of three enzymes that are critical to HydRegen.* Use of these enzymes for production of chemical building-block quninuclidinol at \>10g scale.* Validation of the cost and sustainability metrics for quinuclidinol manufacture.* New IP (enzyme production, biocatalyst formulation, process chemistry).* 'License-ready' bio-based manufacturing route for quinuclidinol that meets the needs for UK-based production (cost, foot-print, productivity, safety, sustainability).

** hydrogen **是牛津大学化学系于2021年分拆出来的项目。我们的愿景：氢技术为更清洁、更安全、更快速、更便宜的化学品生产提供了潜力。对生物学和化学的深刻理解使hydrogen团队能够从生物学中选择最佳成分，并利用它们开发强大的生物技术，解决化学领域的实际问题。我们希望我们的技术在英国化工行业发挥作用，实现雄心勃勃的净零排放目标（到2050年），并越来越多地采用工业生物催化和连续流工艺等技术。目前，我们正在解决制药和特种化学品（如香精和香料）生产中的挑战，并探索我们的技术在大规模、低价值化学品生产中的潜力。** Morra博士小组（诺丁汉大学）**在能够循环H2和H+的新型氢化酶方面拥有专业知识。这些酶对氢很感兴趣，因为它们在“易于生产”和“易于处理”方面具有不同寻常的平衡。Morra博士的团队在酶生产方面拥有专业知识和设备，从早期学术研究到生物反应器中可扩展酶生产的评估（高达100升）。**项目重点：**在这里，我们通过加强和规模化酶生产，并将所产生的酶应用于喹啉酚生产的示范工艺，来解决氢技术生产准备就绪的挑战。喹啉是许多活性药物中重要的化学成分。Morra博士和hydrogen博士在BBSRC-NIBB的资助下于2022年开始合作。该项目使双方合作伙伴都能够实现这种合作的潜力。**项目产出：***降低了三种制氢关键酶的生产成本。*使用这些酶生产化学构件基喹啉，生产规模为100 - 10克。*验证喹喹啉生产的成本和可持续性指标。*新知识产权（酶生产、生物催化剂配方、工艺化学）。*“许可就绪”的喹喹啉生物基生产路线，满足英国生产的需求（成本、足迹、生产率、安全性、可持续性）。