CORE B (Molecular Pathology Core)

摘要/摘要： 分子病理学核心将设在迈阿密大学，由Dr。 Oliver McDonald，医学博士，博士，哥伦比亚大学公共卫生硕士Ladan Fazlollahi博士的合作支持 大学。这一核心为P01调查人员提供了几项基本服务。分子病理学的核心 与迈阿密大学现有的基础设施对接，以协调收集高质量的 组织标本(人和动物)，以便有经验的胃肠道病理学家处理 以玻片为基础的形式(H&E染色、TMA、特殊化学染色、免疫组织化学染色) 适用于组织病理学显微镜检查。核心还注释、存储和跟踪组织 收集和加工时的生物检疫。最重要的是，核心提供专家胃肠 病理会诊和诊断幻灯片审查，包括HE形态、特殊染色解释和 免疫组织化学(IHC)评价。这些基本核心服务是成功执行 P01项目，所有这些项目都需要对Barrett‘s食道(BE)和 食管腺癌(EAC)。BE相关性腺体发育不良的分级、分级和分期 食管腺癌，评估这些病变中的原位蛋白表达，并实施 量化评分系统可以接受与 生物统计学核心。

Abstract/Summary: The Molecular Pathology Core will be located at the University of Miami under the direct supervision of Dr. Oliver McDonald, MD, Ph.D., with collaborative support from Dr. Ladan Fazlollahi, MD, MPH at Columbia University. This Core provides several essential services to P01 investigators. The Molecular Pathology Core interfaces with existing infrastructure at the University of Miami to coordinate the collection of high quality tissue specimens (human and animal) so that an experienced gastrointestinal pathologist can process them into slide-based formats (H&E stains, TMAs, specialized chemical stains, immunohistochemical stains) that are suitable for histopathologic microscopic evaluation. The Core also annotates, stores, and tracks tissue biospecimens as they are collected and processed. Most importantly, the Core provides expert gastrointestinal pathology consultation and diagnostic slide review, including H&E morphology, special stain interpretation, and immunohistochemical (IHC) evaluation. These basic Core services are required for successful execution of P01 projects, all of which require in-depth histopathologic characterization of Barrett’s Esophagus (BE) and esophageal adenocarcinoma (EAC). The grading of BE-associated glandular dysplasia, grading and staging esophageal adenocarcinoma, evaluating of in situ protein expression in these lesions, and implementating of quantitative scoring systems are amenable to statistical tests of significance in collaboration with the Biostatistics Core.