CORE B (Molecular Pathology Core)

CORE B（分子病理学核心）

• 批准号: 10407749
• 负责人: Oliver Gene McDonald
• 金额: $ 13.24万
• 依托单位: UNIVERSITY OF MIAMI SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-07-08 至 2027-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10407749
• 关键词: Animals; Barrett Esophagus; Basic Science; Biostatistics Core; Chemicals; Clinical Research; Collaborations; Collection; Comprehensive Cancer Center; Consultations; Custom; Data; Diagnostic; Doctor of Philosophy; Dysplasia; Ensure; Esophageal Adenocarcinoma; Evaluation; Experimental Pathology; Fee-for-Service Plans; Genetic Transcription; Gold; Histology; Human; In Situ; Individual; Infrastructure; Laboratories; Lesion; Measures; Microscopic; Morphology; Mus; Operative Surgical Procedures; Pathologist; Pathology; Patients; Process; Quality Control; Research; Research Personnel; Resource Sharing; Resources; Sampling; Services; Slide; Specimen; Staging; Stains; Structure; Supervision; System; Testing; Tissue Procurements; Tissue Sample; Tissues; Universities; Work; animal tissue; anticancer research; base; biobank; clinically relevant; cost effective; experience; gastrointestinal; human tissue; in vivo; interest; molecular pathology; mouse model; neoplastic; protein expression; quality assurance; tissue resource; tumorigenesis

Abstract/Summary: The Molecular Pathology Core will be located at the University of Miami under the direct supervision of Dr. Oliver McDonald, MD, Ph.D., with collaborative support from Dr. Ladan Fazlollahi, MD, MPH at Columbia University. This Core provides several essential services to P01 investigators. The Molecular Pathology Core interfaces with existing infrastructure at the University of Miami to coordinate the collection of high quality tissue specimens (human and animal) so that an experienced gastrointestinal pathologist can process them into slide-based formats (H&E stains, TMAs, specialized chemical stains, immunohistochemical stains) that are suitable for histopathologic microscopic evaluation. The Core also annotates, stores, and tracks tissue biospecimens as they are collected and processed. Most importantly, the Core provides expert gastrointestinal pathology consultation and diagnostic slide review, including H&E morphology, special stain interpretation, and immunohistochemical (IHC) evaluation. These basic Core services are required for successful execution of P01 projects, all of which require in-depth histopathologic characterization of Barrett’s Esophagus (BE) and esophageal adenocarcinoma (EAC). The grading of BE-associated glandular dysplasia, grading and staging esophageal adenocarcinoma, evaluating of in situ protein expression in these lesions, and implementating of quantitative scoring systems are amenable to statistical tests of significance in collaboration with the Biostatistics Core.

摘要/总结： 分子病理学中心将设在迈阿密大学，由Dr. 奥利弗麦克唐纳，医学博士，博士，在哥伦比亚大学医学博士Ladan Fazlollahi博士的合作支持下， 大学该核心为P01研究者提供几项基本服务。分子病理学核心 与迈阿密大学现有的基础设施接口，以协调收集高质量的 组织标本（人类和动物），以便有经验的胃肠道病理学家可以处理它们 以载玻片为基础的格式（H&E染色，TMA，专门的化学染色，免疫组织化学染色）， 适用于组织病理学显微镜评价。Core还可以注释、存储和跟踪组织 生物标本，因为他们收集和处理。最重要的是，核心提供专家胃肠 病理学咨询和诊断切片审查，包括H&E形态学，特殊染色解释， 免疫组织化学（IHC）评价。这些基本核心服务是成功执行 P01项目，所有这些项目都需要对Barrett食管（BE）进行深入的组织病理学表征， 食管腺癌（EAC）。BE相关腺体发育不良的分级、分级和分期 食管腺癌，评估这些病变中的原位蛋白表达，并实施 定量评分系统可以与 生物统计学核心。