An Exploris 240 for Metabolomics

项目摘要 匹兹堡大学的健康科学代谢组学和脂质组学核心（HSMLC）要求 资金用于Thermo Fisher Exploris 240质谱仪与Vanquish UHPLC耦合，以扩大 技术能力和产品提供给其用户群。HSMLC成立于2017年，目前服务于 超过100名调查员这些合作范围从按服务收费的客户到 广泛的以赠款为基础的合作。自成立以来，该核心小组已为23项同行审议作出了贡献。 出版物和115份赠款申请，资助率为21%。Exploris 240连接到Vanquish UPLC将补充2018年底购买的现有Thermo Fisher ID-X三杂交体， S10奖。Vanquish-ID-X系统已成功并持续用于脂质组学和 代谢组学自安装以来，其唯一的主要停机时间归因于COVID 19。获得的数据 ID-X为与HSMLC相关的23篇出版物中的8篇做出了贡献。虽然许多研究人员利用 代谢组学和脂质组学的HSMLC，该提案突出了主要兴趣在于 代谢组学Exploris 240的安装将补充ID-X，并允许分离 代谢组学和脂质组学平台，Exploris 240仅用于代谢组学分析， 包括靶向分析、差异分析和13 C示踪研究。在以下方面取得了一些进展： 仪器证明需要这个要求超过其他高分辨率质谱仪，包括（1） 简单的IC内部校准（2）扩大质荷比范围，低至40 m/z（3）极性切换， 高质量准确度和（4）获取非靶向代谢组学的X功能。这些进步将有助于 在较低质量范围内使用MS 2片段进行代谢物鉴定。MS 2片段在40-50 m/z之间 在同位素异构体分析的实施中也是至关重要的， 富集，但富集的位置将需要提供代谢失调的支持数据。 此外，将脂质组学和代谢组学分离到各个平台上将补充最近的 扩大我们的核心员工，并允许方法开发时间，以扩大核心能力和 我们使命的一个更广泛的教育和培训部门的成立，提供我们在LC-MS方面的专业知识 分析，实验规划和培训有兴趣更好地了解质量的合作者 光谱应用。

Project Summary The Health Sciences Metabolomics and Lipidomics Core (HSMLC) at the University of Pittsburgh is requesting funds for a Thermo Fisher Exploris 240 mass spectrometer coupled to a Vanquish UHPLC to expand technological capabilities and offerings to its user base. The HSMLC was founded in 2017 and currently serves over 100 investigators at Pitt and beyond. These collaborations range from fee-for-service customers to extensive grant-based collaborations. Since its inception, the core has contributed to 23 peer reviewed publications and 115 grant submissions with a 21% funding rate. The Exploris 240 coupled to the Vanquish UPLC will complement an existing Thermo Fisher ID-X tribrid that was purchased at the end of 2018 with an S10 award. The Vanquish-ID-X system has been used successfully and continuously for both lipidomics and metabolomics since its installation, with its only major downtime attributed to COVID19. Data obtained on the ID-X contributed to 8 of the 23 publications associated with the HSMLC. While many investigators utilize the HSMLC for metabolomics and lipidomics, this proposal highlights investigators whose primary interest is in metabolomics. Installation of the Exploris 240 will complement the ID-X and allow the separation of metabolomic and lipidomics platforms with the Exploris 240 being solely used for metabolomics analysis that includes targeted profiling, differential analysis and 13C tracer studies. Several advancements in instrumentation justify the need for this request over other high resolution mass spectrometers and include (1) Easy IC—internal calibration (2) expanded mass to charge range, down to 40 m/z (3) polarity switching with high mass accuracy and (4) Acquire X functionality for untargeted metabolomics. These advancements will aid in metabolite identification using MS2 fragments in the lower mass range. MS2 fragments between 40-50 m/z will also be critical in the implementation of isotopomers analysis when not only the number of carbons enriched, but the position of enrichment will be required to provide supporting data for metabolic dysregulation. Furthermore, separation of lipidomics and metabolomics onto individual platforms will complement the recent expansion of our core staff and allow for method development time to expand core capabilities and the inception of a more extensive education and training arm of our mission, to provide our expertise in LC-MS analysis, experimental planning and training to collaborators who are interested in better understanding mass spectrometry applications.