Phenomaster NG Mouse Metabolic Phenotyping System

摘要 在这项S10申请中，需要资金购买新的小鼠代谢表型系统，以 为圣路易斯华盛顿大学许多对新陈代谢感兴趣的研究人员提供服务。我们正在寻找 获得具有以下模块的Phenomaster下一代(NG)小鼠代谢表型系统 测量体力活动、食物摄入量、氧气消耗、二氧化碳产生和其他代谢 参数同时放在16个单独的笼子中。此外，该系统还将提供两台电动汽车 在运动挑战中测量这些参数的跑步机。最后，新系统将是 封闭在环境室中，以控制光线循环，防止环境应激源暴露，以及 将温度控制在3-30°C的范围内。我们已经确定了十几所华盛顿大学 由NIH资助并将成为该系统的主要用户的教职员工。此外，几个次要用户 也被鉴定出。这些教职员工被任命在华盛顿各地的不同部门。 这表明了该机构对新陈代谢的跨学科兴趣，还 强调我们大学的合作性质。PhenoMaster NG系统将安装在 动物模型研究核心，为糖尿病研究中心和营养性肥胖提供服务 华盛顿大学研究中心。目前，该核心使用的PhenoMaster系统有几个 年，使用过时的技术，只有八个笼子，目前保留了几个 提前几个月，用户等待获得访问权限。新系统将大大缩短等待时间 随着它所提供的技术进步，将提高实验的严密性和重复性 在这个系统中进行。系统中包括的附加模块(跑步机和13CO2传感器)将 也为严格描述代谢的特性提供了新的实验机会和终点 表型。成功收购PhenoMaster NG将提高吞吐量、准确性和精确度 一个现有的代谢表型鉴定设施，为校园内多名由NIH资助的研究人员提供服务。

ABSTRACT In this S10 application, funds are requested to purchase a new system for mouse metabolic phenotyping to serve the many researchers interested in metabolism at Washington University in St. Louis. We are seeking to acquire a Phenomaster Next Generation (NG) mouse metabolism phenotyping system with modules for measuring physical activity, food intake, oxygen consumption, carbon dioxide production, and other metabolic parameters concurrently in sixteen individual cages. Moreover, this system will also provide two motorized treadmills for measuring these parameters during exercise challenges. Lastly, the new system will be enclosed in an environmental chamber to control light cycles, prevent environmental stressor exposure, and control the temperature in a range from 3-30ºC. We have identified more than a dozen Washington University faculty members who are NIH funded and will be major users for this system. In addition, several minor users were also identified. These faculty members are appointed in a variety of departments across Washington University, which illustrates the cross-disciplinary interest in metabolism at the institution and also emphasizes the collaborative nature of our university. The PhenoMaster NG system would be housed in the Animal Models Research Core, which serves both the Diabetes Research Center and Nutrition Obesity Research Center at Washington University. Currently, this core uses a PhenoMaster system that is several years old, employs antiquated technology, comprises only eight cages, and currently is reserved several months in advance with users waiting to gain access. The new system would alleviate much of this wait time and with the technologic advances it provides, would improve rigor and reproducibility of the experiments conducted in this system. The additional modules (treadmills and 13CO2 sensors) included in the system will also provide new experimental opportunities and endpoints for rigorous characterization of metabolic phenotypes. Successful acquisition of the PhenoMaster NG will enhance throughput, accuracy, and precision of an existing metabolic phenotyping facility that serves multiple NIH-funded investigators across campus.