Zeiss LSM 980 with Airyscan 2 for Imaging Core Facility

项目概要/摘要 此申请是寻求资金购买蔡司LSM 980共焦与Airyscan 2将被安置在一个 格鲁吉亚州立大学（GSU）文理学院的全校成像核心设施， 支持22个NIH资助的研究项目，3个NSF资助的研究项目，和3个其他研究基金会- 为16个用户群体（28个项目，9个主要用户和7个次要用户）资助研究项目。这些项目 包括癌症研究、离子通道调节、神经发育和 神经基因组学，疼痛管理的神经生物学，社会行为和心理健康，神经系统疾病， 喂养和体重控制，神经炎症，分娩和微生物对大脑发育，尿酸盐 代谢、代谢综合征、炎症性肠病、脂肪吞噬、认知和能量稳态， 等建议的高端，完全集成的交钥匙共焦系统，具有更高的分辨率，增强 灵敏度和成像速度的提高被所有用户认为是成像核心的理想系统 满足关键需求的设施。新的共焦系统将用于联邦资助的团体进行 在共享使用的基础上进行生物医学研究，以最大限度地支持NIH资助的基础， 翻译、生物医学和神经行为研究。将所提出的共焦系统并入到 成像核心设施将提高成本效益，确保平稳和安全的运行，鼓励 独立研究人员、研究小组、部门/研究所和学院之间的均衡共享 和大学水平的研究中心，并促进多学科合作。成像核心设施有 两个使用针孔的老化激光扫描共聚焦系统，Zeiss LSM 700（10年以上）和LSM 780（6年以上） 旧）。它们正被各种生物医学研究人员大量用于细胞和组织成像 样本跨越GSU和附近机构的多个主要部门/研究所。的年度服务 这两个系统的协议将在不久的将来停止由蔡司和更换零件 不会得到保证。系统的任何意外故障都将对系统造成巨大的破坏。 正在进行的NIH资助的研究项目在时间和研究资金方面。此外，收购这样一个 高端共焦系统将提供一种独特工具来扩展其执行高速成像的能力， 大大降低了光毒性，并提高了对细胞和组织切片样品成像的分辨率和灵敏度。 这将是一个伟大的除了设施，以保证继续支持各种NIH资助的项目。它将 还帮助研究人员推进和加快他们的研究项目，超越他们目前的限制。 该设施有专门的空间和关键人员到位，以支持采购和日常运作， 拟议的共聚焦系统以及得到强有力机构支持的可行和可持续的财政计划 确保拟议共聚焦系统的长期运行和维护，以最大限度地支持 并对当前和未来NIH资助的具有尖端成像能力的研究项目产生影响。

Project Summary/Abstract This application is to seek funds to purchase a Zeiss LSM 980 confocal with Airyscan 2 to be housed in a university-wide Imaging Core Facility in the College of Arts and Sciences at Georgia State University (GSU) to support 22 NIH-funded research projects, 3 NSF funded research projects, and 3 other research foundation- funded research projects for 16 user groups (28 projects, 9 Major Users and 7 Minor Users). These projects include ongoing investigations in the field of cancer research, ion channel modulation, neural development and neurogenomics, neurobiology of pain management, social behavior and mental health, neurological disorders, feeding and body weight control, neuroinflammation, parturition and microbes on brain development, urate metabolism, metabolic syndrome, inflammatory bowel disease, lipophagy, cognition and energy homeostasis, etc. The proposed high-end, fully integrated turnkey confocal system with improved resolution, enhanced sensitivity, and increased imaging speed is identified by all the users to be an ideal system for the Imaging Core Facility to meet critical needs. The new confocal system will be used by federally funded groups conducting biomedical research on a shared-use basis to maximize the supports to the NIH-funded projects in basic, translational, biomedical, and neurobehavioral research. The incorporation of the proposed confocal system into the Imaging Core Facility will promote cost-effectiveness, ensure a smooth and safe operation, encourage balanced sharing among independent researchers, research groups, departments/institutes, and College-level & University-level research centers, and boost multidisciplinary collaborations. The Imaging Core Facility has two aging laser scanning confocal systems using pinholes, Zeiss LSM 700 (10+ year old) and LSM 780 (6+ year old). They are being heavily used by a diverse group of biomedical investigators for imaging cell and tissue samples span multiple major departments/institutes at GSU and nearby institutions. The annual service agreements for both systems are going to be discontinued by Zeiss in the near future and replacement parts are not going to be guaranteed. Any unexpected breakdown of the systems will cause tremendous disruptions to the ongoing NIH-funded research projects in terms of time and research funds. Besides, the acquisition of such a high-end confocal system will provide a unique tool to extend their ability to perform high-speed imaging with greatly reduced phototoxicity and improved resolution and sensitivity for imaging cell and tissue section samples. It will be a great addition to the facility to guarantee the continued support to varied NIH-funded projects. It will also help the investigators to advance and accelerate their research projects beyond their current limitations. The facility has dedicated space and critical personnel in place to support the acquisition and daily operation of the proposed confocal system as well as a viable and sustainable financial plan with strong institutional support to ensure the long-term operation and maintenance of the proposed confocal system to maximize the support and impact to current and future NIH-funded research projects with cutting-edge imaging capabilities.