Zeiss LSM 980 with Airyscan 2 for Imaging Core Facility

项目摘要/摘要 此应用程序是寻求资金，以购买蔡司LSM 980共焦与AiryScan 2，以容纳在 佐治亚州立大学(GSU)文理学院的大学成像核心设施 支持22个NIH资助的研究项目，3个NSF资助的研究项目，以及3个其他研究基金会- 资助16个用户组的研究项目(28个项目，9个主要用户和7个次要用户)。这些项目 包括正在进行的癌症研究、离子通道调制、神经发育和 神经基因组学，疼痛管理的神经生物学，社会行为和心理健康，神经障碍， 喂养和体重控制，神经炎症，分娩和微生物对大脑发育的影响，尿酸盐 代谢，代谢综合征，炎症性肠病，吞脂性，认知和能量平衡， 等。拟议的高端、完全集成的交钥匙共焦系统具有更高的分辨率、增强的 灵敏度和成像速度的提高被所有用户认为是成像核心的理想系统 满足关键需求的设施。新的共焦系统将由联邦资助的团体使用， 在共享使用的基础上进行生物医学研究，以最大限度地支持NIH资助的基础、 翻译、生物医学和神经行为研究。将拟议的共焦系统纳入 成像核心设施将促进成本效益，确保顺利和安全运行，鼓励 在独立研究人员、研究小组、部门/研究所和学院级别之间实现均衡共享 和大学级的研究中心，促进多学科合作。成像核心设备具有 两个使用针孔的老化激光扫描共焦系统，Zeiss LSM 700(使用10年)和LSM 780(使用6年 旧的)。它们正被各种生物医学研究人员大量用于细胞和组织的成像 样本涉及格拉斯哥州立大学和附近机构的多个主要部门/研究所。年度服务 蔡司将在不久的将来终止这两个系统的协议，更换部件 不会得到保证。任何意想不到的系统故障都会对 在时间和研究资金方面，正在进行的NIH资助的研究项目。此外，收购这样一个 高端共焦系统将提供一种独特的工具，以扩展他们执行高速成像的能力 大大降低了光毒性，提高了成像细胞和组织切片样品的分辨率和灵敏度。 这将是对该设施的一个极大的补充，以保证对NIH资助的各种项目的持续支持。会的 还可以帮助研究人员推进和加快他们的研究项目，使其超越目前的限制。 该设施有专门的空间和关键人员，以支持采购和日常运营 拟议的协调中心系统以及具有强有力机构支助的可行和可持续的财务计划 确保拟议的共焦系统长期运行和维护，以最大限度地提供支助 以及对目前和未来由美国国立卫生研究院资助的具有尖端成像能力的研究项目的影响。

Project Summary/Abstract This application is to seek funds to purchase a Zeiss LSM 980 confocal with Airyscan 2 to be housed in a university-wide Imaging Core Facility in the College of Arts and Sciences at Georgia State University (GSU) to support 22 NIH-funded research projects, 3 NSF funded research projects, and 3 other research foundation- funded research projects for 16 user groups (28 projects, 9 Major Users and 7 Minor Users). These projects include ongoing investigations in the field of cancer research, ion channel modulation, neural development and neurogenomics, neurobiology of pain management, social behavior and mental health, neurological disorders, feeding and body weight control, neuroinflammation, parturition and microbes on brain development, urate metabolism, metabolic syndrome, inflammatory bowel disease, lipophagy, cognition and energy homeostasis, etc. The proposed high-end, fully integrated turnkey confocal system with improved resolution, enhanced sensitivity, and increased imaging speed is identified by all the users to be an ideal system for the Imaging Core Facility to meet critical needs. The new confocal system will be used by federally funded groups conducting biomedical research on a shared-use basis to maximize the supports to the NIH-funded projects in basic, translational, biomedical, and neurobehavioral research. The incorporation of the proposed confocal system into the Imaging Core Facility will promote cost-effectiveness, ensure a smooth and safe operation, encourage balanced sharing among independent researchers, research groups, departments/institutes, and College-level & University-level research centers, and boost multidisciplinary collaborations. The Imaging Core Facility has two aging laser scanning confocal systems using pinholes, Zeiss LSM 700 (10+ year old) and LSM 780 (6+ year old). They are being heavily used by a diverse group of biomedical investigators for imaging cell and tissue samples span multiple major departments/institutes at GSU and nearby institutions. The annual service agreements for both systems are going to be discontinued by Zeiss in the near future and replacement parts are not going to be guaranteed. Any unexpected breakdown of the systems will cause tremendous disruptions to the ongoing NIH-funded research projects in terms of time and research funds. Besides, the acquisition of such a high-end confocal system will provide a unique tool to extend their ability to perform high-speed imaging with greatly reduced phototoxicity and improved resolution and sensitivity for imaging cell and tissue section samples. It will be a great addition to the facility to guarantee the continued support to varied NIH-funded projects. It will also help the investigators to advance and accelerate their research projects beyond their current limitations. The facility has dedicated space and critical personnel in place to support the acquisition and daily operation of the proposed confocal system as well as a viable and sustainable financial plan with strong institutional support to ensure the long-term operation and maintenance of the proposed confocal system to maximize the support and impact to current and future NIH-funded research projects with cutting-edge imaging capabilities.