"Clinical Trials" on a Premature Vascular Aging-on-a-Chip Model

摘要 哈钦森-吉尔福德早衰症(HGPS)是一种导致早产和早衰的遗传性疾病。 在生理衰老过程中，孕激素的积累也会发生。我们的胎教项目旨在 优化由患者来源的成纤维细胞(FBS)生成的仿生HGPS芯片上系统，平滑 肌肉细胞(SMC)和内皮细胞(ECs)，允许应用相关的循环拉伸来执行 “临床试验”或告知HGPS患者的临床试验设计。本副刊进一步旨在发展 简化实验设计的内部协议，因为它们与数据生成、收集、 分析、存储以及存储到MPS-DB中，具体到父母补助金和最多 对MPS-DB有意义的方式。我们预计，我们将能够成功地制定这些简化的 在1年补充项目期内的协议，允许我们开始存储任何数据 在父项目的Y2年内或之后生成，而不必 在实验室生成的数据与MPS-DB之间建立联系方面投入更多的专门努力。

Abstract Hutchinson-Gilford progeria syndrome (HGPS) is a genetic disorder that results in premature and accelerated aging, while accumulation of progerin also occurs during physiological aging. Our paratal project seeks to optimize a biomimetic HGPS-on-a-chip system generated with patient-derived fibroblasts (FBs), smooth muscle cells (SMCs), and endothelial cells (ECs) that allow application of relevant cyclic stretch for performing ‘clinical trials’ or informing clinical trial designs for HGPS patients. This supplement further aims at developing in-house protocols for streamlining experimental designs as they relate to data-generation, collection, analyses, storage, as well as deposition into the MPS-Db, specific to the parental grant and in the most meaningful way to the MPS-Db. We anticipate that we will be able to succeed in formulating these streamlined protocols within the 1-year supplement project period that will allow us to both start depositing any data generated in Y2 of the parental project or beyond as soon as they become available, without having to spending additional dedicated efforts in bridging the laboratory-generated data with the MPS-Db.