"Clinical Trials" on a Premature Vascular Aging-on-a-Chip Model

摘要 Hutchinson-Gilford早衰综合征（HGPS）是一种遗传性疾病， 老化，而早老蛋白的积累也发生在生理老化过程中。我们的paratal项目旨在 优化用患者来源的成纤维细胞（FB）生成的仿生HGPS-on-a-chip系统，平滑 肌肉细胞（SMC）和内皮细胞（EC），其允许应用相关的周期性拉伸以执行 “临床试验”或告知HGPS患者的临床试验设计。本附录旨在进一步发展 简化实验设计的内部协议，因为它们涉及数据生成，收集， 分析，存储，以及沉积到MPS-Db，具体到父母补助金，在大多数情况下， 对MPS-Db有意义的方式。我们预计，我们将能够成功地制定这些精简的 在1年的补充项目期间，这将使我们都开始存放任何数据的协议 在父母项目的第二年或之后，一旦它们可用， 花费额外的专门努力将实验室生成的数据与MPS-Db连接起来。

Abstract Hutchinson-Gilford progeria syndrome (HGPS) is a genetic disorder that results in premature and accelerated aging, while accumulation of progerin also occurs during physiological aging. Our paratal project seeks to optimize a biomimetic HGPS-on-a-chip system generated with patient-derived fibroblasts (FBs), smooth muscle cells (SMCs), and endothelial cells (ECs) that allow application of relevant cyclic stretch for performing ‘clinical trials’ or informing clinical trial designs for HGPS patients. This supplement further aims at developing in-house protocols for streamlining experimental designs as they relate to data-generation, collection, analyses, storage, as well as deposition into the MPS-Db, specific to the parental grant and in the most meaningful way to the MPS-Db. We anticipate that we will be able to succeed in formulating these streamlined protocols within the 1-year supplement project period that will allow us to both start depositing any data generated in Y2 of the parental project or beyond as soon as they become available, without having to spending additional dedicated efforts in bridging the laboratory-generated data with the MPS-Db.