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Center for Sub-Cellular Genomics

亚细胞基因组学中心

基本信息

批准号：
10457892
负责人：
JAMES H EBERWINE
金额：
$ 250万
依托单位：
UNIVERSITY OF PENNSYLVANIA
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-09-01 至 2024-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10457892
关键词：
Address Alzheimer&apos s Disease Attention Automobile Driving Awareness Biological Assay Biological Models Bipolar Disorder Cell Nucleus Cell physiology Cells Cellular biology Characteristics Chromatin Communities Complex Data Dendrites Depressive disorder Devices Disease Education and Outreach Educational workshop Functional disorder Genetic Transcription Genome Genomics High-Throughput Nucleotide Sequencing Human Individual Informatics Institution Investigation Learning Liquid substance Mass Spectrum Analysis Measurement Measures Memory Mental Depression Methods Mitochondria Modeling Molecular Molecular Biology Motivation Nanotechnology Neurodegenerative Disorders Neurons Nuclear Optical Methods Optics Organelles Output Pathway interactions Phenotype Philadelphia Physiology Process Proteins RNA Regulation Research Project Grants Resolution Risk Rodent Role Schizophrenia Signal Transduction Structure Synapses System Techniques Technology Tissues Training Translations Traumatic Brain Injury Variant Work autism spectrum disorder base deep learning epigenomics exosome experience flexibility functional genomics genome-wide human disease informatics tool lipidomics macromolecule metabolomics multimodality nanoscale neuropsychiatric disorder new technology novel outreach response subcellular targeting technology development technology validation transcriptome welfare whole genome

项目摘要

A cell is a highly complex system with distributed molecular physiologies in structured sub- cellular compartments whose interplay with the nuclear genome determine the functional characteristics of the cell. A classic example of distributed genomic processes is found in neurons. Learning and memory requires modulation of individual synapses through RNA localization, localized translation, and localized metabolites such as those from dendritic mitochondria. Dendrites of neurons integrate distributed synaptic signals into both electrical and nuclear transcriptional response. Dysfunction of these distributed genomic functions in neurons can result in a broad spectrum of neuropsychiatric diseases such as bipolar and depressive disorders, autism, among others. Understanding complex genomic interactions within a single cell requires new technologies: we need nano-scale ability to make genome-wide measurements at highly localized compartments and to effect highly localized functional genomic manipulations, especially in live tissues. To address this need, we propose to establish a Center for Sub-Cellular Genomics using neurons as model systems. The center will develop new optical and nanotechnology approaches to isolate sub-cellular scale components for genomic, metabolomics, and lipidomic analyses. The center will also develop new mass spectrometry methods, molecular biology methods, and informatics models to create a platform technology for sub-cellular genomics.

细胞是一个高度复杂的系统，在结构化的子系统中具有分布式分子生理学。与核基因组的相互作用决定功能的细胞区室细胞的特性。分布式基因组过程的一个经典例子是神经元学习和记忆需要通过RNA调节单个突触局部化、局部化翻译和局部化代谢物，例如来自树突状细胞的那些代谢物，线粒体神经元的树突将分布的突触信号整合到电和神经元中。核转录反应。神经元中这些分布的基因组功能的功能障碍可导致广泛的神经精神疾病，如双相情感障碍和抑郁症失调症自闭症等等了解单个细胞内复杂的基因组相互作用需要新技术：我们需要纳米级的能力，在高度局部化的区域进行全基因组测量并实现高度定位的功能性基因组操作，尤其是在活组织中。到为了满足这一需求，我们建议建立一个使用神经元的亚细胞基因组学中心 as model模型systems系统.该中心将开发新的光学和纳米技术方法，用于基因组学、代谢组学和脂质组学分析分离的亚细胞规模组分。的中心还将开发新的质谱分析方法，分子生物学方法，信息学模型，为亚细胞基因组学创建平台技术。

项目成果

期刊论文数量（9）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Modeling and optimization of parallelized immunomagnetic nanopore sorting for surface marker specific isolation of extracellular vesicles from complex media.

DOI：
10.1038/s41598-023-39746-7
发表时间：
2023-08-16
期刊：
SCIENTIFIC REPORTS
影响因子：
4.6
作者：
Lin, Andrew A.;Shen, Hanfei;Spychalski, Griffin;Carpenter, Erica L.;Issadore, David
通讯作者：
Issadore, David

Label-Free Multitarget Separation of Particles and Cells under Flow Using Acoustic, Electrophoretic, and Hydrodynamic Forces.

DOI：
10.1021/acs.analchem.1c00312
发表时间：
2021-05
期刊：
Analytical chemistry
影响因子：
7.4
作者：
Yupan Wu;Rajarshi Chattaraj;Yukun Ren;Hongyuan Jiang;Daeyeon Lee
通讯作者：
Yupan Wu;Rajarshi Chattaraj;Yukun Ren;Hongyuan Jiang;Daeyeon Lee

Parallelized immunomagnetic nanopore sorting: modeling, scaling, and optimization of surface marker specific isolation of extracellular vesicles from complex media.

并行免疫磁性纳米孔分选：从复杂介质中表面标记物特异性分离细胞外囊泡的建模、缩放和优化。

DOI：
10.21203/rs.3.rs-2913647/v1
发表时间：
2023
期刊：
Research square
影响因子：
0
作者：
Lin,AndrewA;Shen,Hanfei;Spychalski,Griffin;Carpenter,EricaL;Issadore,David
通讯作者：
Issadore,David

Scaling up the throughput of microfluidic droplet-based materials synthesis: A review of recent progress and outlook.

扩大基于微流控液滴的材料合成的通量：近期进展和展望的回顾。

DOI：
10.1063/5.0049897
发表时间：
2021
期刊：
Applied physics reviews
影响因子：
15
作者：
Wu,Jingyu;Yadavali,Sagar;Lee,Daeyeon;Issadore,DavidA
通讯作者：
Issadore,DavidA

Ultrasensitive Single Extracellular Vesicle Detection Using High Throughput Droplet Digital Enzyme-Linked Immunosorbent Assay.

DOI：
10.1021/acs.nanolett.2c00274
发表时间：
2022-06-08
期刊：
NANO LETTERS
影响因子：
10.8
作者：
Yang, Zijian;Atiyas, Yasemin;Shen, Hanfei;Siedlik, Michael J.;Wu, Jingyu;Beard, Kryshawna;Fonar, Gennadiy;Dolle, Jean Pierre;Smith, Douglas H.;Eberwine, James H.;Meaney, David F.;Issadore, David A.
通讯作者：
Issadore, David A.