Fusion protein based biopesticides for sustainable crop production

该项目旨在从融合蛋白中开发新的生物杀虫剂，这些融合蛋白可以传递杀虫毒素并穿过目标昆虫的肠道。该项目的结果将是创造有针对性的融合蛋白杀虫剂;这需要开发经济生产的工艺，加快表达速率，优化相关的发酵和冷冻干燥。具体目标是：（i）“第一候选”融合蛋白的生产（ii）表达和发酵的增加（iii）下游加工和冷冻干燥的研究（iv）用于最大功效和贮存稳定性的递送制剂（v）进行所有温室/田间试验以确定在最终用户条件下的功效，提供用于提交注册的数据，包括对非靶生物的影响（vi）应用这些方法来简化后续候选融合蛋白的开发

This project aims to develop novel bio insecticides from fusion proteins that deliver insecticidal toxins and across the target insect gut. The results of the project will be the creation of targeted fusion protein insecticides; this requires the development of processes for their economic production, acceleration of expression rate, and optimisation of associated fermenting and freeze drying. The detailed objectives are: (i) production of a "first candidate" fusion protein (ii) increase in expression and fermentation (iii) investigation of downstream processing and freeze drying (iv) delivery formulation for maximum efficacy and shelf stability (v) conduct all glasshouse/field trials to establish efficacy under end user conditions providing data for submission for registration, including effects on non target organisms (vi) application of these processes to streamline the development of follow up candidate fusion proteins