Flow Cytometry

项目摘要/摘要-流式细胞仪共享资源 联合国儿童基金会流式细胞术共享资源(Flow Cytometer)支持基础癌症和转化性癌症 研究通过提供具有成本效益的全方位服务的流式细胞仪服务和高速无菌细胞分选， 包括对病人样本的分析，向联合国儿童基金会成员和非成员用户提供。教务主任 Jennifer Gillette博士(CMO)负责管理资源，该资源由一名全职核心技术总监运营， Wade Johnson博士。联合国排雷行动研究管理局管理该资源，该资源提供几个 各种类型的流式细胞仪服务，包括用于复杂多小组分析和单细胞分析的仪器 分选到微孔板中进行克隆、聚合酶链式反应和RNA测序分析。预订、计费和使用 通过一个集中的在线系统进行跟踪。此外，该资源还管理流式细胞仪数据 机构的分析合同，单个实验室可以降价购买。资源总监 和工作人员与联合国儿童基金会研究方案密切合作，开发新的方法，促进创新 基础研究和翻译研究目标。此外，该资源还积极促进和传播信息 关于新的流式细胞术和数据分析技术，通过维护最新的网页和提供 在联东综合团会议和务虚会上的介绍。最后，资源总监和工作人员积极参与 通过培训和参加几个研究生级别的课程，联合国儿童基金会继续为联合国儿童基金会的教育任务服务。在.期间 在上一个5年项目期间，来自3个联合国儿童特派团研究方案的58名联合国儿童特派团成员 占总资源使用量的75%。此外，资源还支助了146份出版物和128份 同行评审的赠款。

Project Summary/Abstract - FLOW CYTOMETRY SHARED RESOURCE The UNMCCC Flow Cytometry Shared Resource (Flow Cytometry) supports basic and translational cancer research by providing cost-effective, full service flow cytometry services and high-speed sterile sorting of cells, including the analysis of patient samples, to UNMCCC members and non-member users. Faculty Director Jennifer Gillette, Ph.D. (CMO) supervises the Resource, which is operated by a full-time core technical director, Wade Johnson, Ph.D. The UNMCCC Research Administration manages the Resource, which provides several types of flow cytometry services including instrumentation for complex multi-panel analysis as well as single cell sorting into microplate wells for cloning, PCR, and RNA-sequencing analysis. Reservations, billing and usage are tracked through a centralized on-line system. Additionally, the Resource manages the flow cytometry data analysis contract for the institution, which individual labs can buy into at a reduced price. The Resource Director and staff work closely with UNMCCC research programs to develop new methodologies that promote innovative basic and translational research goals. Moreover, the Resource actively promotes and disseminates information about new flow cytometry and data analysis techniques by maintaining an up-to-date web page and by giving presentations at UNMCCC meetings and retreats. Finally, the Resource Director and staff actively engage in the education mission of the UNMCCC through training and participation in several graduate level courses. During the previous 5-year project period, 58 UNMCCC members from 3 UNMCCC Research Programs were responsible for 75% of total Resource usage. Additionally, the Resource supported 146 publications and 128 peer-reviewed grants.