Flow Cytometry

项目总结/摘要-流式细胞术共享资源 UNMCCC流式细胞术共享资源（流式细胞术）支持基础和转化癌症 通过提供具有成本效益的全方位流式细胞仪服务和细胞的高速无菌分选， 包括对病人样本的分析，提供给联合国儿童基金会成员和非成员用户。课程主任 Jennifer Gillette博士(CMO)监督由全职核心技术总监运营的资源， 韦德约翰逊博士联合国气候变化协调中心研究管理处负责管理该资源，该资源提供若干 流式细胞仪服务类型包括复杂的多面板分析仪器以及单细胞 分选到微板威尔斯孔中用于克隆、PCR和RNA测序分析。预订、计费和使用 通过一个集中的在线系统进行跟踪。此外，资源管理流式细胞术数据 分析合同的机构，其中个别实验室可以购买以降低价格。资源总监 和工作人员与联合国儿童基金会的研究项目密切合作，开发新的方法，促进创新 基础和转化研究目标。此外，资源中心还积极宣传和传播信息， 有关新的流式细胞术和数据分析技术，通过维护一个最新的网页，并给予 在联合国儿童基金会会议和务虚会上作介绍。最后，资源主任和工作人员积极参与 通过培训和参加几个研究生课程，执行联合国儿童基金会的教育使命。期间 在前5年的项目期间，来自3个联合国气候变化中心研究项目的58名联合国气候变化中心成员， 占总资源使用量的75%。此外，该资源还支持了146份出版物和128份 同行评审赠款。

Project Summary/Abstract - FLOW CYTOMETRY SHARED RESOURCE The UNMCCC Flow Cytometry Shared Resource (Flow Cytometry) supports basic and translational cancer research by providing cost-effective, full service flow cytometry services and high-speed sterile sorting of cells, including the analysis of patient samples, to UNMCCC members and non-member users. Faculty Director Jennifer Gillette, Ph.D. (CMO) supervises the Resource, which is operated by a full-time core technical director, Wade Johnson, Ph.D. The UNMCCC Research Administration manages the Resource, which provides several types of flow cytometry services including instrumentation for complex multi-panel analysis as well as single cell sorting into microplate wells for cloning, PCR, and RNA-sequencing analysis. Reservations, billing and usage are tracked through a centralized on-line system. Additionally, the Resource manages the flow cytometry data analysis contract for the institution, which individual labs can buy into at a reduced price. The Resource Director and staff work closely with UNMCCC research programs to develop new methodologies that promote innovative basic and translational research goals. Moreover, the Resource actively promotes and disseminates information about new flow cytometry and data analysis techniques by maintaining an up-to-date web page and by giving presentations at UNMCCC meetings and retreats. Finally, the Resource Director and staff actively engage in the education mission of the UNMCCC through training and participation in several graduate level courses. During the previous 5-year project period, 58 UNMCCC members from 3 UNMCCC Research Programs were responsible for 75% of total Resource usage. Additionally, the Resource supported 146 publications and 128 peer-reviewed grants.