Clemson University Genomics and Bioinformatics Facility (CUGBF)

项目摘要/摘要 基因组学和生物信息学是当今生命科学研究的主流。一大障碍 对于来自IDEA州的初级教员来说，缺乏最先进的设施和培训机会 通常在NIH有大量资金的州发现。这通常会阻碍产生 NIH R01资金具有竞争力所需的初步数据。《实施》 测量和解释基因动力学和基因组结构的先进工具对 真核病原体的研究为治疗干预的发展提供依据。基因组水平 分析和生物信息学是一个发展中的领域，迅速出现新的标准和 能够洞察真核生物学基本原理的技术。大多数生命科学 研究人员在使用生物信息学和基因组学方面缺乏经验。因此，我们 提议继续聘用科布雷第一阶段下聘用的生物信息学专家，雇用一名 额外的湿实验室技术人员来处理我们快速增加的负荷，然后支持基因组学， 通过克莱姆森的计算和生物信息基础设施和教育推广 大学基因组学和生物信息学设施(CUGBF)在第二阶段。增强的 CUGBF由三个相互补充的部分组成：1)具有基因组学的分子实验室 和下一代测序(NGS)资源，2)具有高性能的生物信息学实验室 性能计算资源；3)克莱姆森大学的人员和设备 人类遗传学-基因组科学实验室中心(CUCHG-GSL，由 人类遗传学的眼镜蛇)。具备这三项功能后，增强型CUGBF可提供 核酸提取和下一代测序综合服务包 对复杂的数据进行分析和解释。通过这个模型，CUGBF设计了， 执行并教育EPIC TIS和CU教职员工，以实现基因组学和 将生物信息学纳入他们的研究计划。科布雷二期堆芯的主要目标 优化建议是继续提升现有的物质和人力资本，用于 基因组学和计算基础设施，并整合基因组数据和 将计算资源用于德克萨斯大学和加州大学的教职员工研究计划。

Project Summary/Abstract Genomics and bioinformatics are the currency in today’s Life Science research. A major obstacle for junior faculty from IDeA states is the lack of state-of-the-art facilities and training opportunities commonly found in states with substantial NIH funding. This often hinders the generation of preliminary data necessary to be competitive for NIH R01 funding. The implementation of advanced tools to measure and interpret gene dynamics and genomic architecture is vital to the study of eukaryotic pathogens for the development of therapeutic intervention. Genomic level analyses and bioinformatics are a developing field, with rapidly emerging new standards and technologies that provide insight into the fundamentals of eukaryotic biology. Most life science researchers are not experienced in the use of bioinformatics and genomics. Therefore, we propose the continuation of the bioinformatics specialists hired under COBRE Phase I, hire an additional wet lab technician to handle our rapidly increasing load and then bolster the genomics, computational and bioinformatic infrastructure and educational outreach via the Clemson University Genomics and Bioinformatics Facility (CUGBF) throughout Phase II. The enhanced CUGBF is structured into three complementary parts: 1) a molecular laboratory with genomics and next-generation sequencing (NGS) resources, 2) a bioinformatics laboratory with high performance computing resources, & 3) the personnel and equipment at the Clemson University Center for Human Genetics- Genomics Sciences laboratory (CUCHG-GSL which is supported by a COBRE in Human Genetics). Having all three allows the enhanced CUGBF to offer a comprehensive service package from extraction and next-generation sequencing of nucleic acid to the complex data analysis and interpretation. Through this model, CUGBF has designed, executed, and educated EPIC TIs and CU faculty to enable the integration of genomics and bioinformatics into their research programs. The Primary goal of this COBRE Phase II core enhancement proposal is to continue to enhance the current physical and human capital for genomics and computational infrastructure of the CUGBF and integrate genomic data and computational resources into the TI’s and CU faculty research programs.