Clemson University Genomics and Bioinformatics Facility (CUGBF)

项目概要/摘要 基因组学和生物信息学是当今生命科学研究的主流。一个主要障碍 对于来自IDEA州的初级教师来说，缺乏最先进的设施和培训机会 通常在国家卫生研究院有大量资金的州发现。这往往阻碍了 初步数据必须是有竞争力的NIH R 01资金。执行 先进的工具来测量和解释基因动态和基因组结构是至关重要的， 研究真核病原体以开发治疗干预。基因组水平 分析和生物信息学是一个发展中的领域，新标准迅速出现， 提供深入了解真核生物学基础的技术。大多数生命科学 研究人员在使用生物信息学和基因组学方面缺乏经验。所以我们 建议继续在COBRE第一阶段聘用生物信息学专家， 额外的湿实验室技术人员来处理我们迅速增加的工作量，然后支持基因组学， 通过克莱姆森的计算和生物信息基础设施和教育推广 大学基因组学和生物信息学设施（CUGBF）在整个第二阶段。增强的 CUGBF分为三个互补的部分：1）基因组学分子实验室 和下一代测序（NGS）资源，2）生物信息学实验室， 性能计算资源，以及3）克莱姆森大学的人员和设备 人类遗传学中心-基因组学科学实验室（CUCHG-GSL，由 人类遗传学中的COBRE）。拥有这三个允许增强的CUGBF提供 从核酸提取到下一代测序的综合服务包 复杂的数据分析和解释。通过这个模型，CUGBF设计了， 执行，并教育EPIC TI和CU教师，使基因组学和 将生物信息学融入他们的研究计划中COBRE第二阶段核心的主要目标是 加强建议是继续加强目前的物质和人力资本， CUGBF的基因组学和计算基础设施，并整合基因组数据， 计算资源到TI和CU的教师研究计划。

Project Summary/Abstract Genomics and bioinformatics are the currency in today’s Life Science research. A major obstacle for junior faculty from IDeA states is the lack of state-of-the-art facilities and training opportunities commonly found in states with substantial NIH funding. This often hinders the generation of preliminary data necessary to be competitive for NIH R01 funding. The implementation of advanced tools to measure and interpret gene dynamics and genomic architecture is vital to the study of eukaryotic pathogens for the development of therapeutic intervention. Genomic level analyses and bioinformatics are a developing field, with rapidly emerging new standards and technologies that provide insight into the fundamentals of eukaryotic biology. Most life science researchers are not experienced in the use of bioinformatics and genomics. Therefore, we propose the continuation of the bioinformatics specialists hired under COBRE Phase I, hire an additional wet lab technician to handle our rapidly increasing load and then bolster the genomics, computational and bioinformatic infrastructure and educational outreach via the Clemson University Genomics and Bioinformatics Facility (CUGBF) throughout Phase II. The enhanced CUGBF is structured into three complementary parts: 1) a molecular laboratory with genomics and next-generation sequencing (NGS) resources, 2) a bioinformatics laboratory with high performance computing resources, & 3) the personnel and equipment at the Clemson University Center for Human Genetics- Genomics Sciences laboratory (CUCHG-GSL which is supported by a COBRE in Human Genetics). Having all three allows the enhanced CUGBF to offer a comprehensive service package from extraction and next-generation sequencing of nucleic acid to the complex data analysis and interpretation. Through this model, CUGBF has designed, executed, and educated EPIC TIs and CU faculty to enable the integration of genomics and bioinformatics into their research programs. The Primary goal of this COBRE Phase II core enhancement proposal is to continue to enhance the current physical and human capital for genomics and computational infrastructure of the CUGBF and integrate genomic data and computational resources into the TI’s and CU faculty research programs.