Nanomedicine-Driven Strategies to Repair Peripheral Nerve Injuries

项目摘要 神经系统的正常运作对我们的生活质量非常重要。神经 在我们的身体内，从脊柱发出分支，可以延伸到一米远。周围神经损伤 是一种常见的情况，在大多数情况下都很容易痊愈。然而，末端器官超过~18英寸远 来自神经体的，极易受到不良的再神经结果和长期损害的影响。A Main 原因是轴突生长速度较慢(1-2 mm/天或1英寸/月)。一些组织，如 骨骼肌，可以保持18-24个月的神经再支配。然而，目前的干预策略 是有限的，大多数伤势都会自然解决。此应用程序旨在开发一种能够 促进更快的轴突生长，并保持肌肉的健康状态，直到发生神经再生。 这一应用主要集中在组织纳米转染介导的非病毒基因载体的传递上，以诱导 分子的变化和细胞的重新编程。将货物就地运送到受伤地点的能力开启 以精确医学方法为基础的基因和细胞疗法超越神经损伤的途径。 F99阶段的完成奠定了坚实的智力、技术和专业基础 该奖项的博士后(K00)阶段。在K00阶段，培训了解细胞和基因是如何- 基础疗法影响神经发育和神经退行性疾病将发展知识， 成为一名独立调查员所必需的专业知识和技能。

PROJECT ABSTRACT Appropriate functioning of the nervous system is tremendously important to our quality of life. The nerves within our body branch from the spinal column and can extend up to one meter away. Peripheral nerve injuries are a common occurrence and can readily heal in most cases. However, end-organs beyond ~18 inches away from the nerve body, are highly susceptible to poor reinnervation outcomes and long-term detriments. A main reason for this being the slow growth rate of the axons (1-2mm/day or 1 inch/month). Some tissues, such as skeletal muscle, can remain ready for reinnervation for 18-24 months. However, current intervention strategies are limited, and most injuries are left to resolve naturally. This application aims to develop a strategy capable of promoting quicker neurite outgrowth and preserving the muscle in a healthy state until reinnervation can occur. This application focuses on tissue-nanotransfection mediated delivery of non-viral gene cargo to induce molecular changes and to reprogram cells. The ability to deliver cargos locally to the site of injury opens avenues beyond nerve injury for gene and cell-based therapeutics with a precision medicine approach. Completion of the F99 phase sets a strong intellectual, technical and professional foundation for the postdoctoral (K00) phase of this award. During the K00 phase, training in understanding how cell and gene- based therapies impact neurodevelopmental and neurodegenerative conditions will develop knowledge, expertise, and skills essential to becoming an independent investigator.