Biophysics Core

生物物理学核心

• 批准号: 10508448
• 负责人: Gregory B Melikian
• 金额: $ 77.98万
• 依托单位: SEATTLE CHILDREN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-06-22 至 2027-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10508448
• 关键词: Acylation; Automobile Driving; Beds; Behavior; Biological; Biological Models; Biophysics; Capsid; Cells; Cellular Structures; Complex; Coupled; Detection; Development; Educational workshop; Environment; Equipment; Event; Fluorescence; Fluorescence Microscopy; Fluorescence Resonance Energy Transfer; Goals; HIV; HIV-1; Hydroxyl Radical; Image; In Vitro; Investigation; Joints; Label; Life Cycle Stages; Machine Learning; Macromolecular Complexes; Mass Spectrum Analysis; Membrane Proteins; Mission; Nuclear Import; Nucleic Acids; Ohio; Persons; Phase; Photobleaching; Primer Extension; Process; Protein Dynamics; Proteins; RNA; RNA Probes; Research Personnel; Resolution; Resources; Services; Spectrum Analysis; Techniques; Technology; Testing; Training; Universities; Viral; Visit; biophysical analysis; biophysical properties; biophysical techniques; cellular imaging; crosslink; insight; instrumentation; new technology; novel imaging technology; particle; protein complex; quantitative imaging; response; single molecule; spectroscopic imaging; stoichiometry; structural biology; tool

ABSTRACT, CORE 3 Biophysical methods, such as single-molecule techniques, quantitative imaging/spectroscopy and native mass spectrometry (nMS) are powerful tools to gain mechanistic insights into key dynamic interactions and processes occurring during the early and late steps of the HIV-1 lifecycle. The goal of Core 3 (Biophysics Core) is to provide requisite instrumentation, expertise and training to augment the B-HIVE capabilities across all three center projects by enabling cutting-edge studies of protein-protein, protein-nucleic acid and protein-membrane interactions in living cells and in model systems. To accomplish these goals we will: (1) Provide advanced fluorescence microscopy capabilities to visualize early and late events of the HIV life cycle (Melikian); (2) provide advanced capabilities in single-molecule biophysics to probe dynamics of proteins, nucleic acids and their complexes (Millar & Musier-Forsyth); and (3) provide methods for biophysical characterization of macromolecular complexes (Musier-Forsyth and Wysocki). A critical part of the Core 3 mission is training the users remotely or in person, through visits to the labs of the Core co-leads, workshops and videoconferences. To accomplish this goal, the core will provide basic imaging, single-molecule and nMS services using established pipelines to all B-HIVE projects, as needed, implement advanced techniques and engage in long-term projects through collaborative efforts with the B-HIVE investigators, and develop novel technologies in response to the needs of Projects 1-3. 1

摘要，核心 3 生物物理方法，例如单分子技术、定量成像/光谱学和天然质量 光谱测定 (nMS) 是获得关键动态相互作用和过程的机械见解的强大工具 发生在 HIV-1 生命周期的早期和晚期阶段。 Core 3（生物物理学核心）的目标是提供 必要的仪器、专业知识和培训，以增强所有三个中心的 B-HIVE 能力 通过实现蛋白质-蛋白质、蛋白质-核酸和蛋白质-膜的前沿研究来开展项目 活细胞和模型系统中的相互作用。为了实现这些目标，我们将：（1）提供先进的 荧光显微镜功能可可视化 HIV 生命周期的早期和晚期事件（Melikian）； (2) 提供 单分子生物物理学的先进能力，可探测蛋白质、核酸及其动力学 复合物（Millar & Musier-Forsyth）； (3) 提供生物物理表征的方法 大分子复合物（Musier-Forsyth 和 Wysocki）。 Core 3 任务的一个关键部分是培训 通过访问核心联合领导的实验室、研讨会和视频会议，远程或亲自与用户交流。 为了实现这一目标，该核心将使用已建立的技术提供基本成像、单分子和 nMS 服务。 根据需要向所有 B-HIVE 项目输送管道，实施先进技术并参与长期项目 通过与 B-HIVE 研究人员的合作，开发新技术来应对 项目1-3的需求。 1