Biophysics Core
生物物理学核心
基本信息
- 批准号:10508448
- 负责人:
- 金额:$ 77.98万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2022
- 资助国家:美国
- 起止时间:2022-06-22 至 2027-03-31
- 项目状态:未结题
- 来源:
- 关键词:AcylationAutomobile DrivingBedsBehaviorBiologicalBiological ModelsBiophysicsCapsidCellsCellular StructuresComplexCoupledDetectionDevelopmentEducational workshopEnvironmentEquipmentEventFluorescenceFluorescence MicroscopyFluorescence Resonance Energy TransferGoalsHIVHIV-1Hydroxyl RadicalImageIn VitroInvestigationJointsLabelLife Cycle StagesMachine LearningMacromolecular ComplexesMass Spectrum AnalysisMembrane ProteinsMissionNuclear ImportNucleic AcidsOhioPersonsPhasePhotobleachingPrimer ExtensionProcessProtein DynamicsProteinsRNARNA ProbesResearch PersonnelResolutionResourcesServicesSpectrum AnalysisTechniquesTechnologyTestingTrainingUniversitiesViralVisitbiophysical analysisbiophysical propertiesbiophysical techniquescellular imagingcrosslinkinsightinstrumentationnew technologynovel imaging technologyparticleprotein complexquantitative imagingresponsesingle moleculespectroscopic imagingstoichiometrystructural biologytool
项目摘要
ABSTRACT, CORE 3
Biophysical methods, such as single-molecule techniques, quantitative imaging/spectroscopy and native mass
spectrometry (nMS) are powerful tools to gain mechanistic insights into key dynamic interactions and processes
occurring during the early and late steps of the HIV-1 lifecycle. The goal of Core 3 (Biophysics Core) is to provide
requisite instrumentation, expertise and training to augment the B-HIVE capabilities across all three center
projects by enabling cutting-edge studies of protein-protein, protein-nucleic acid and protein-membrane
interactions in living cells and in model systems. To accomplish these goals we will: (1) Provide advanced
fluorescence microscopy capabilities to visualize early and late events of the HIV life cycle (Melikian); (2) provide
advanced capabilities in single-molecule biophysics to probe dynamics of proteins, nucleic acids and their
complexes (Millar & Musier-Forsyth); and (3) provide methods for biophysical characterization of
macromolecular complexes (Musier-Forsyth and Wysocki). A critical part of the Core 3 mission is training the
users remotely or in person, through visits to the labs of the Core co-leads, workshops and videoconferences.
To accomplish this goal, the core will provide basic imaging, single-molecule and nMS services using established
pipelines to all B-HIVE projects, as needed, implement advanced techniques and engage in long-term projects
through collaborative efforts with the B-HIVE investigators, and develop novel technologies in response to the
needs of Projects 1-3.
1
摘要,核心3
生物物理方法,如单分子技术、定量成像/光谱学和自然质量
光谱分析(nMS)是获得对关键动态相互作用和过程的机械见解的强大工具
发生在HIV-1生命周期的早期和晚期。核心3(生物物理学核心)的目标是提供
必要的仪器、专业知识和培训,以增强所有三个中心的B-HIVE能力
通过实现蛋白质-蛋白质,蛋白质-核酸和蛋白质-膜的前沿研究,
活细胞和模型系统中的相互作用。为了实现这些目标,我们将:(1)提供先进的
荧光显微镜的能力,以可视化艾滋病毒生命周期的早期和晚期事件(Melikian);(2)提供
单分子生物物理学的先进能力,以探测蛋白质,核酸及其
复合物(Millar & Musier-Forsyth);和(3)提供用于生物物理表征的方法,
大分子复合物(Musier-Forsyth和Wysocki)。核心3号使命的一个关键部分是训练
通过访问核心共同领导人的实验室、研讨会和视频会议,远程或亲自与用户进行交流。
为了实现这一目标,核心将提供基本的成像,单分子和nMS服务,
根据需要,为所有B-HIVE项目提供管道,实施先进技术并参与长期项目
通过与B-HIVE研究人员的合作努力,并开发新技术,以应对
项目1-3的需求。
1
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Gregory B Melikian其他文献
Gregory B Melikian的其他文献
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{{ truncateString('Gregory B Melikian', 18)}}的其他基金
Molecular Interactions of HIV-1 with the Nuclear Pore Complex
HIV-1 与核孔复合物的分子相互作用
- 批准号:
10241258 - 财政年份:2019
- 资助金额:
$ 77.98万 - 项目类别:
Molecular Interactions of HIV-1 with the Nuclear Pore Complex
HIV-1 与核孔复合物的分子相互作用
- 批准号:
10462620 - 财政年份:2019
- 资助金额:
$ 77.98万 - 项目类别:
Inhibition of viral entry by interferon-induced proteins
干扰素诱导蛋白抑制病毒进入
- 批准号:
10418696 - 财政年份:2018
- 资助金额:
$ 77.98万 - 项目类别:
Inhibition of viral entry by interferon-induced proteins
干扰素诱导蛋白抑制病毒进入
- 批准号:
10190798 - 财政年份:2018
- 资助金额:
$ 77.98万 - 项目类别:
Imaging of Single HIV-1 Uncoating and Transport to the nucleus
单个 HIV-1 脱壳和转运至细胞核的成像
- 批准号:
9354023 - 财政年份:2017
- 资助金额:
$ 77.98万 - 项目类别:
Functional Characterization of the Hepatitis C Virus E1-E2 Glycoproteins
丙型肝炎病毒 E1-E2 糖蛋白的功能表征
- 批准号:
7522862 - 财政年份:2009
- 资助金额:
$ 77.98万 - 项目类别:
Functional Characterization of the Hepatitis C Virus E1-E2 Glycoproteins
丙型肝炎病毒 E1-E2 糖蛋白的功能表征
- 批准号:
8116923 - 财政年份:2009
- 资助金额:
$ 77.98万 - 项目类别:
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