Biophysics Core

生物物理学核心

• 批准号: 10508448
• 负责人: Gregory B Melikian
• 金额: $ 77.98万
• 依托单位: SEATTLE CHILDREN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-06-22 至 2027-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10508448
• 关键词: Acylation; Automobile Driving; Beds; Behavior; Biological; Biological Models; Biophysics; Capsid; Cells; Cellular Structures; Complex; Coupled; Detection; Development; Educational workshop; Environment; Equipment; Event; Fluorescence; Fluorescence Microscopy; Fluorescence Resonance Energy Transfer; Goals; HIV; HIV-1; Hydroxyl Radical; Image; In Vitro; Investigation; Joints; Label; Life Cycle Stages; Machine Learning; Macromolecular Complexes; Mass Spectrum Analysis; Membrane Proteins; Mission; Nuclear Import; Nucleic Acids; Ohio; Persons; Phase; Photobleaching; Primer Extension; Process; Protein Dynamics; Proteins; RNA; RNA Probes; Research Personnel; Resolution; Resources; Services; Spectrum Analysis; Techniques; Technology; Testing; Training; Universities; Viral; Visit; biophysical analysis; biophysical properties; biophysical techniques; cellular imaging; crosslink; insight; instrumentation; new technology; novel imaging technology; particle; protein complex; quantitative imaging; response; single molecule; spectroscopic imaging; stoichiometry; structural biology; tool

ABSTRACT, CORE 3 Biophysical methods, such as single-molecule techniques, quantitative imaging/spectroscopy and native mass spectrometry (nMS) are powerful tools to gain mechanistic insights into key dynamic interactions and processes occurring during the early and late steps of the HIV-1 lifecycle. The goal of Core 3 (Biophysics Core) is to provide requisite instrumentation, expertise and training to augment the B-HIVE capabilities across all three center projects by enabling cutting-edge studies of protein-protein, protein-nucleic acid and protein-membrane interactions in living cells and in model systems. To accomplish these goals we will: (1) Provide advanced fluorescence microscopy capabilities to visualize early and late events of the HIV life cycle (Melikian); (2) provide advanced capabilities in single-molecule biophysics to probe dynamics of proteins, nucleic acids and their complexes (Millar & Musier-Forsyth); and (3) provide methods for biophysical characterization of macromolecular complexes (Musier-Forsyth and Wysocki). A critical part of the Core 3 mission is training the users remotely or in person, through visits to the labs of the Core co-leads, workshops and videoconferences. To accomplish this goal, the core will provide basic imaging, single-molecule and nMS services using established pipelines to all B-HIVE projects, as needed, implement advanced techniques and engage in long-term projects through collaborative efforts with the B-HIVE investigators, and develop novel technologies in response to the needs of Projects 1-3. 1

摘要，核心3 生物物理方法，如单分子技术、定量成像/光谱学和天然质量 光谱分析(NMS)是获得对关键动态交互和过程的机械性洞察的强大工具 发生在艾滋病毒-1生命周期的早期和后期。核心3(生物物理学核心)的目标是提供 必要的仪器、专业知识和培训，以增强B-HIVE在所有三个中心的能力 促进对蛋白质-蛋白质、蛋白质-核酸和蛋白质-膜的前沿研究的项目 活细胞和模型系统中的相互作用。为实现这些目标，我们将：(1)提供先进的 显示艾滋病毒生命周期早期和晚期事件的荧光显微镜能力(梅利肯)；(2)提供 在单分子生物物理方面的先进能力，以探测蛋白质、核酸及其 络合物(Millar和Musier-Forsyth)；和(3)提供生物物理表征的方法 大分子络合物(Musier-Forsyth和Wysocki)。核心3任务的一个关键部分是培训 远程或面对面的用户，通过访问核心共同领导的实验室、讲习班和视频会议。 为了实现这一目标，核心将提供基本的成像、单分子和NMS服务 根据需要为所有B-HIVE项目铺设管道，实施先进技术并参与长期项目 通过与B-HIVE调查人员的合作，并开发新技术来应对 项目1-3的需要。 1