Biophysics Core

生物物理学核心

• 批准号: 10508448
• 负责人: Gregory B Melikian
• 金额: $ 77.98万
• 依托单位: SEATTLE CHILDREN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-06-22 至 2027-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10508448
• 关键词: Acylation; Automobile Driving; Beds; Behavior; Biological; Biological Models; Biophysics; Capsid; Cells; Cellular Structures; Complex; Coupled; Detection; Development; Educational workshop; Environment; Equipment; Event; Fluorescence; Fluorescence Microscopy; Fluorescence Resonance Energy Transfer; Goals; HIV; HIV-1; Hydroxyl Radical; Image; In Vitro; Investigation; Joints; Label; Life Cycle Stages; Machine Learning; Macromolecular Complexes; Mass Spectrum Analysis; Membrane Proteins; Mission; Nuclear Import; Nucleic Acids; Ohio; Persons; Phase; Photobleaching; Primer Extension; Process; Protein Dynamics; Proteins; RNA; RNA Probes; Research Personnel; Resolution; Resources; Services; Spectrum Analysis; Techniques; Technology; Testing; Training; Universities; Viral; Visit; biophysical analysis; biophysical properties; biophysical techniques; cellular imaging; crosslink; insight; instrumentation; new technology; novel imaging technology; particle; protein complex; quantitative imaging; response; single molecule; spectroscopic imaging; stoichiometry; structural biology; tool

ABSTRACT, CORE 3 Biophysical methods, such as single-molecule techniques, quantitative imaging/spectroscopy and native mass spectrometry (nMS) are powerful tools to gain mechanistic insights into key dynamic interactions and processes occurring during the early and late steps of the HIV-1 lifecycle. The goal of Core 3 (Biophysics Core) is to provide requisite instrumentation, expertise and training to augment the B-HIVE capabilities across all three center projects by enabling cutting-edge studies of protein-protein, protein-nucleic acid and protein-membrane interactions in living cells and in model systems. To accomplish these goals we will: (1) Provide advanced fluorescence microscopy capabilities to visualize early and late events of the HIV life cycle (Melikian); (2) provide advanced capabilities in single-molecule biophysics to probe dynamics of proteins, nucleic acids and their complexes (Millar & Musier-Forsyth); and (3) provide methods for biophysical characterization of macromolecular complexes (Musier-Forsyth and Wysocki). A critical part of the Core 3 mission is training the users remotely or in person, through visits to the labs of the Core co-leads, workshops and videoconferences. To accomplish this goal, the core will provide basic imaging, single-molecule and nMS services using established pipelines to all B-HIVE projects, as needed, implement advanced techniques and engage in long-term projects through collaborative efforts with the B-HIVE investigators, and develop novel technologies in response to the needs of Projects 1-3. 1

摘要，核心3 生物物理方法，如单分子技术、定量成像/光谱学和自然质量 光谱分析（nMS）是获得对关键动态相互作用和过程的机械见解的强大工具 发生在HIV-1生命周期的早期和晚期。核心3（生物物理学核心）的目标是提供 必要的仪器、专业知识和培训，以增强所有三个中心的B-HIVE能力 通过实现蛋白质-蛋白质，蛋白质-核酸和蛋白质-膜的前沿研究， 活细胞和模型系统中的相互作用。为了实现这些目标，我们将：（1）提供先进的 荧光显微镜的能力，以可视化艾滋病毒生命周期的早期和晚期事件（Melikian）;（2）提供 单分子生物物理学的先进能力，以探测蛋白质，核酸及其 复合物（Millar & Musier-Forsyth）;和（3）提供用于生物物理表征的方法， 大分子复合物（Musier-Forsyth和Wysocki）。核心3号使命的一个关键部分是训练 通过访问核心共同领导人的实验室、研讨会和视频会议，远程或亲自与用户进行交流。 为了实现这一目标，核心将提供基本的成像，单分子和nMS服务， 根据需要，为所有B-HIVE项目提供管道，实施先进技术并参与长期项目 通过与B-HIVE研究人员的合作努力，并开发新技术，以应对 项目1-3的需求。 1