High Throughput Platform for the Discovery of GMP Compatible Stem Cell Manufacturing Protocols

在可控和可重复的方式下制造细胞是再生医学中的一个关键挑战。我们将开发一种集成平台技术，用于快速识别新的、高效的干细胞扩增和分化方案，这些方案经过优化，可转化为临床级生产。几项创新技术将结合在一起，进行高通量GMP兼容筛选，以发现新的细胞生产协议。这些协议将被转移到一个放大的生物过程中，在那里将使用一种新的细胞成像技术来监测细胞性能。成功的协议将被转换为GMP制造过程，以进行最终验证。通过这种方式，安全、可靠、成本效益高的GMP验证方案将被快速发现，从而极大地降低再生细胞疗法的开发成本和时间。

The manufacture of cells in a controlled and reproducible manner is a critical challenge in regenerative medicine. We will develop an integrated platform technology for the rapid identification of novel, efficient stem cell expansion and differentiation protocols that are optimised for translation to clinical grade manufacturing. Several innovative technologies will be combined to perform high throughput GMP compatible screens to discover novel cell production protocols. These protocols will be transferred to a scaled-up bioprocess where a novel cell imaging technology will be utilised to monitor cell performance. Successful protocols will be translated to a GMP manufacturing process for final validation. In this way, safe, robust, cost-effective GMP validated protocols will be rapidly discovered, greatly reducing the cost and time for development of regenerative cell therapeutics.