Proteomics

摘要：蛋白质组学共享资源 蛋白质组学SR（PROT）是一个国家的最先进的设施，带来了前沿的蛋白质组学研究LCCC 教师。它是一个完善的共享资源（SR），为实验设计提供咨询， 为LCCC成员提供样品制备、质谱（MS）和广泛的数据分析。所指令 专家的工作人员，SR是全方位的服务，允许用户与项目，但很少有经验，以了解范围 数据采集机会。SR还具有允许更有经验的用户访问的工具 到昂贵的、维护良好的质谱仪。核心工作人员和专家用户扩展了 利用创新技术，如MIB/MS分析细胞范围内的激酶活性。贡献包括 验证和表征癌症相关药物靶标，定量蛋白质组和信号网络 对新兴疗法的反应变化，研究激酶组的动态调节，描述如何 特异性途径在整体上重塑癌症蛋白质组，而不依赖于基因表达的变化， 确定蛋白质相互作用网络如何在癌症中失调。 仪器包括;最先进的质谱仪包括：Thermo Lumos、Thermo QExactive HF- X、Thermo QExactive Bioderma、Thermo QExactive HF、Thermo Orbitrap Velos和Sciex 5800 MALDI/TOF/TOF。所有这些都是由LCCC部分购买或租赁的，包括过去四年内的四个。的 购买和维护目前的PROT SR仪器超出了任何单个实验室的能力。 LCCC的投资使多个实验室能够获得高端技术。PROT的成本 SR服务通常比可比的私人公司或其他学术机构低25%， LCCC会员获得额外折扣，使该设施具有相当的成本效益。LCCC要求 CCSG的135，301美元，占总运营预算的21%; SR使用的48%由 LCCC教师。

ABSTRACT: PROTEOMICS SHARED RESOURCE The Proteomics SR (PROT) is a state-of-the-art facility that brings cutting edge proteomics research to LCCC faculty. It is a well-established shared resource (SR) that provides consultation for the design of experiments, sample preparation, mass spectrometry (MS) and extensive data analysis for LCCC members. Directed by an expert staff, the SR is full service allowing users with projects but little experience to understand the range of data acquisition opportunities. The SR also has the instrumentation that allows more experienced users access to expensive, well maintained mass spectrometers. Core staff and expert users have extended the capabilities with innovative techniques such as the MIB/MS analysis of cell wide kinase activity. Contributions include validating and characterizing cancer-relevant drug targets, quantifying proteome and signaling network changes in response to emerging therapeutics, studying the dynamic regulation of the kinome, describing how specific pathways globally remodel cancer proteomes independent of changes in gene expression, and determining how protein interaction networks are dysregulated in cancer. Instrumentation includes; state-of-the-art mass spectrometers include: Thermo Lumos, Thermo QExactive HF- X, Thermo QExactive Biopharma, Thermo QExactive HF, Thermo Orbitrap Velos and Sciex 5800 MALDI/TOF/TOF. All were partially purchased or leased by LCCC including four within the last four years. The purchase and maintenance of the current PROT SR instruments are beyond the means of any individual lab. LCCC investments have made it possible for multiple labs to access high end technology. The cost of PROT SR services are generally lower by 25% than comparable private company or other academic institutions and LCCC members receive an additional discount making the facility quite cost effective. The LCCC is requesting $135,301 from the CCSG, which represents 21% of the total operating budget; 48% of the SR usage is by LCCC faculty.