Determining the role of afadin in tubular development and maintenance in the kidney

确定阿法丁在肾小管发育和维护中的作用

• 批准号: 10543403
• 负责人: Isabel A Alejandra Lopez-Garcia
• 金额: $ 5.18万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-01 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10543403
• 关键词: 3-Dimensional; Adherens Junction; Apical; Architecture; Automobile Driving; Binding; Cells; Central cord canal structure; Chronic Kidney Failure; Data; Defect; Development; Dialysis procedure; Epithelial; Evolution; F-Actin; Filtration; Future; Goals; Grant; Heel; Image; Implant; In Vitro; Intercellular Junctions; Kidney; Kidney Transplantation; Knock-out; Knockout Mice; Knowledge; Label; Lead; Liquid substance; Maintenance; Mediating; Modeling; Molecular; Morphogenesis; Mus; Nephrons; Organoids; Patients; Process; Proteins; Regulation; Renal Replacement Therapy; Renal function; Renal tubule structure; Research; Role; Scaffolding Protein; Signal Transduction; Structure; System; Testing; Therapeutic; Tissues; Tube; Tubular formation; Work; afadin; base; burden of illness; clinically relevant; design; follow-up; global health; improved; in vitro Model; in vivo; in vivo Model; innovation; mouse model; nephrogenesis; novel therapeutic intervention; novel therapeutics; renal epithelium; replacement tissue; tissue regeneration; trafficking

Project Summary/Abstract Chronic kidney disease (CKD) poses an important global disease burden with limited therapeutic options. Novel therapeutic strategies to improve renal function include implanting patient-derived kidney organoids onto the native kidneys. However, an inability to connect organoid tubules with the host’s kidney tubules presents a major problem that remains to be solved. Currently, the molecular mechanisms driving interconnection between tubules and their lumens are poorly understood. Filling this gap in knowledge is critical to advance work with implantable renal replacement tissues. Our previous studies have shown that mice lacking afadin, a cytoskeletal scaffolding protein, from renal epithelial tubules, have a defect in lumen continuity. The goal of this project is to determine how lumen connection/fusion occurs within tubules and determine the molecular mechanism by which afadin promotes lumen fusion and maintenance in renal epithelia. We hypothesize that lumen fusion requires cellular rearrangements and that these are facilitated by afadin-mediated regulation of cell-cell contacts. Here, I propose to use well-defined in vitro and in vivo models to: (1) Determine the mechanism of lumen fusion after the onset of de novo lumenogenesis, (2) Identify the afadin domains required for lumen fusion, and (3) Determine the role of afadin in lumen maintenance in vitro and in an in vivo inducible knockout mouse model. The knowledge obtained from these studies will enable a better design of epithelial structures that have the ability to form and maintain a continuous lumen for future use as implantable therapeutics.

项目总结/文摘