Core C: IgSeq and NGS Core
Core C: IgSeq and NGS Core
批准号:
10549607
负责人:
Jiwon Lee
金额:
$36.36万
依托单位国家:
美国
项目类别:
财政年份:
2011
资助国家:
美国
项目状态:
未结题
起止时间:
2011-08-01 至 2028-03-31
关键词:
AntibodiesAntibody RepertoireAntibody ResponseAntigensAttentionB cell repertoireB-LymphocytesB-cell receptor repertoire sequencingDNA Sequencing FacilityDataData AnalysesDevelopmentElderlyEngineeringExposure toFundingGoalsHemagglutininHumanHumoral ImmunitiesImmuneIndividualInfantInfectionInfluenzaInfluenza HemagglutininInfluenza vaccinationInfrastructureInvestigationLengthLiquid ChromatographyMediatingMemory B-LymphocyteMolecularMonitorMonoclonal AntibodiesNeuraminidaseOnline SystemsPlasmablastPlayPopulationProteomicsRecombinantsResearch PersonnelResearch SupportResolutionResourcesRoleSamplingSeasonsSequence AnalysisSeriesSerumServicesShapesStructureTechnologyTimeTranscriptVaccinationVaccinesViralVirus DiseasesWorkage groupanalytical toolcollegedata repositorydatabase querydesignimprintinfluenza virus vaccinenext generation sequencingprofessorprogramsresponseseasonal influenzasuccesstandem mass spectrometrytooltranscriptomicsvaccination strategyyoung adult
中文摘要
摘要
英文摘要
ABSTRACT
A principal goal of the Program Project is to chart the changes in influenza antibody repertoire following
vaccination or infection and to build on those results to direct immunogen design efforts. Core C, the Ig-Seq
and NGS Core, will serve the overall goals of the Program Project by providing high-throughput antibody
repertoire profiling services. Specifically, it will provide in-depth NextGen Sequencing (NGS) of antibody
transcripts from B cells (BCR-Seq) and quantitative high-resolution proteomic analysis of serum antibody
molecules (Ig-Seq). From these sequence analyses, the Core will generate full-length antibody sequences for
recombinant expression and characterizations as monoclonal antibodies.The Core will also serve all Projects
and Cores of the Program Project by providing a centralized data repository, management of all antibody
sequence data, robust and consistent data analysis. The specific aims of Core C are: (1) provide the NGS
service to define the B cell repertoire; (2) provide the proteomic-based antibody analysis service to define
serum antibody repertoire; (3) provide a centralized data repository and analysis for antibody sequences
generated by all the Projects and Cores; and (4) expand and optimize the provided services. The Ig-Seq and
NGS Core is critical for the success of the Program Project, offering high-throughput tools for deep and
comprehensive monitoring of changes in three distinct components of antibody repertoires: serum antibody
molecules, memory B cells, and plasmablasts.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Understanding Early-life Influenza Antibody Repertoire Imprinting Through Infection or Vaccination
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批准号:10215742
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项目类别:
-
资助金额:$27.42万
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财政年份:2019
-
负责人:Jiwon Lee
-
依托单位:
Understanding the impact of imprinted serum antibody repertoire on subsequent vaccine responses
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批准号:10271749
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项目类别:
-
资助金额:$26.24万
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财政年份:2016
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负责人:Jiwon Lee
-
依托单位:
Understanding the impact of imprinted serum antibody repertoire on subsequent vaccine responses
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批准号:10460275
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项目类别:
-
资助金额:$26.24万
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财政年份:2016
-
负责人:Jiwon Lee
-
依托单位:
Understanding the impact of imprinted serum antibody repertoire on subsequent vaccine responses
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批准号:10647708
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项目类别:
-
资助金额:$26.24万
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财政年份:2016
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负责人:Jiwon Lee
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依托单位:
海外基金