Core C: IgSeq and NGS Core

核心 C：IgSeq 和 NGS 核心

• 批准号: 10549607
• 负责人: Jiwon Lee
• 金额: $ 36.36万
• 依托单位: BOSTON CHILDREN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-08-01 至 2028-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10549607
• 关键词: Antibodies; Antibody Repertoire; Antibody Response; Antigens; Attention; B cell repertoire; B-Lymphocytes; B-cell receptor repertoire sequencing; DNA Sequencing Facility; Data; Data Analyses; Development; Elderly; Engineering; Exposure to; Funding; Goals; Hemagglutinin; Human; Humoral Immunities; Immune; Individual; Infant; Infection; Influenza; Influenza Hemagglutinin; Influenza vaccination; Infrastructure; Investigation; Length; Liquid Chromatography; Mediating; Memory B-Lymphocyte; Molecular; Monitor; Monoclonal Antibodies; Neuraminidase; Online Systems; Plasmablast; Play; Population; Proteomics; Recombinants; Research Personnel; Research Support; Resolution; Resources; Role; Sampling; Seasons; Sequence Analysis; Series; Serum; Services; Shapes; Structure; Technology; Time; Transcript; Vaccination; Vaccines; Viral; Virus Diseases; Work; age group; analytical tool; college; data repository; database query; design; imprint; influenza virus vaccine; next generation sequencing; professor; programs; response; seasonal influenza; success; tandem mass spectrometry; tool; transcriptomics; vaccination strategy; young adult

ABSTRACT A principal goal of the Program Project is to chart the changes in influenza antibody repertoire following vaccination or infection and to build on those results to direct immunogen design efforts. Core C, the Ig-Seq and NGS Core, will serve the overall goals of the Program Project by providing high-throughput antibody repertoire profiling services. Specifically, it will provide in-depth NextGen Sequencing (NGS) of antibody transcripts from B cells (BCR-Seq) and quantitative high-resolution proteomic analysis of serum antibody molecules (Ig-Seq). From these sequence analyses, the Core will generate full-length antibody sequences for recombinant expression and characterizations as monoclonal antibodies.The Core will also serve all Projects and Cores of the Program Project by providing a centralized data repository, management of all antibody sequence data, robust and consistent data analysis. The specific aims of Core C are: (1) provide the NGS service to define the B cell repertoire; (2) provide the proteomic-based antibody analysis service to define serum antibody repertoire; (3) provide a centralized data repository and analysis for antibody sequences generated by all the Projects and Cores; and (4) expand and optimize the provided services. The Ig-Seq and NGS Core is critical for the success of the Program Project, offering high-throughput tools for deep and comprehensive monitoring of changes in three distinct components of antibody repertoires: serum antibody molecules, memory B cells, and plasmablasts.

摘要