Core C: IgSeq and NGS Core

摘要 该计划项目的主要目标是绘制流感抗体库的变化， 疫苗接种或感染，并建立在这些结果的指导免疫原设计的努力。核心C，Ig-Seq 和NGS核心，将通过提供高通量抗体服务于该计划项目的总体目标 曲目分析服务。具体来说，它将提供深入的NextGen测序（NGS）抗体 B细胞转录物（BCR-Seq）和血清抗体的定量高分辨率蛋白质组学分析 分子（Ig-Seq）。从这些序列分析中，核心将产生全长抗体序列，用于 重组表达和表征为单克隆抗体。核心也将服务于所有项目 通过提供一个集中的数据存储库，管理所有抗体， 序列数据，强大和一致的数据分析。核心C的具体目标是：（1）提供国家一般服务 定义B细胞库的服务;（2）提供基于蛋白质组学的抗体分析服务， 血清抗体库;（3）提供集中的抗体序列数据库和分析 由所有项目和核心产生;以及（4）扩展和优化所提供的服务。Ig-Seq和 NGS Core是该计划项目成功的关键，它为深度和 全面监测抗体库三种不同成分的变化：血清抗体 分子、记忆B细胞和浆母细胞。

ABSTRACT A principal goal of the Program Project is to chart the changes in influenza antibody repertoire following vaccination or infection and to build on those results to direct immunogen design efforts. Core C, the Ig-Seq and NGS Core, will serve the overall goals of the Program Project by providing high-throughput antibody repertoire profiling services. Specifically, it will provide in-depth NextGen Sequencing (NGS) of antibody transcripts from B cells (BCR-Seq) and quantitative high-resolution proteomic analysis of serum antibody molecules (Ig-Seq). From these sequence analyses, the Core will generate full-length antibody sequences for recombinant expression and characterizations as monoclonal antibodies.The Core will also serve all Projects and Cores of the Program Project by providing a centralized data repository, management of all antibody sequence data, robust and consistent data analysis. The specific aims of Core C are: (1) provide the NGS service to define the B cell repertoire; (2) provide the proteomic-based antibody analysis service to define serum antibody repertoire; (3) provide a centralized data repository and analysis for antibody sequences generated by all the Projects and Cores; and (4) expand and optimize the provided services. The Ig-Seq and NGS Core is critical for the success of the Program Project, offering high-throughput tools for deep and comprehensive monitoring of changes in three distinct components of antibody repertoires: serum antibody molecules, memory B cells, and plasmablasts.