i-Bacillus: Adapting Bacillus licheniformis for 21st century IB applications

地衣芽孢杆菌是生产工业酶的首选宿主，包括用于洗涤剂的蛋白酶、用于食品和生物燃料的淀粉酶和纤维素酶。为了提高其遗传学/实用性，我们的主要目标是使用SynBio工具来提高地衣芽孢杆菌内源和外源酶生产的经济性，以便通过领先的最终用户进行开发。基因组传递技术将扩增/靶向基因到高水平/可预测表达的位置，克服与非靶向整合相关的问题。基于核酸酶的基因组编辑将a)解决通过系统生物学鉴定的应激条件下宿主代谢的产量妥协方面；b)利用原位蛋白质工程来改善内源性酶的功能，包括在操作条件下的耐热性和最佳活性。在这些领域的成功将降低成本，提高地衣芽孢杆菌生产的工业酶的多功能性和效率。

Bacillus licheniformis is a preferred host for the production of industrial enzymes, including proteasesfor detergent and amylases and cellulases for food and biofuels. To advance its genetics/utility our keyobjective is to deploy SynBio tools to improve endogenous and heterologous enzyme productioneconomics in B. licheniformis for exploitation via leading end users. Genome delivery technologies willamplify/target genes to locations validated for high-level/predictable expression, overcoming issuesassociated with non-targeted integration. Nuclease-based genome editing will a) address yield-compromising aspects of host metabolism under stress conditions identified through systems biologyand b) capitalize on in situ protein engineering to improve endogenous enzyme function includingthermotolerance and optimal activity under operating conditions. Success in these areas will reduce thecost and improve the versatility and efficiency of industrial enzymes produced in B. licheniformis.