i-Bacillus: Adapting Bacillus licheniformis for 21st century IB applications

i-Bacillus：使地衣芽孢杆菌适应 21 世纪 IB 应用

• 批准号: 102498
• 金额: $ 50.53万
• 依托单位: INGENZA LIMITED
• 依托单位国家: 英国
• 项目类别: Collaborative R&D
• 财政年份: 2016
• 资助国家: 英国
• 起止时间: 2016 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=102498
• 关键词: Bacillus; Adapting; licheniformis; 21st; century

Bacillus licheniformis is a preferred host for the production of industrial enzymes, including proteasesfor detergent and amylases and cellulases for food and biofuels. To advance its genetics/utility our keyobjective is to deploy SynBio tools to improve endogenous and heterologous enzyme productioneconomics in B. licheniformis for exploitation via leading end users. Genome delivery technologies willamplify/target genes to locations validated for high-level/predictable expression, overcoming issuesassociated with non-targeted integration. Nuclease-based genome editing will a) address yield-compromising aspects of host metabolism under stress conditions identified through systems biologyand b) capitalize on in situ protein engineering to improve endogenous enzyme function includingthermotolerance and optimal activity under operating conditions. Success in these areas will reduce thecost and improve the versatility and efficiency of industrial enzymes produced in B. licheniformis.

地衣芽孢杆菌是生产工业酶的优选宿主，包括用于洗涤剂的蛋白酶和用于食品和生物燃料的淀粉酶和纤维素酶。为了推进其遗传学/实用性，我们的主要目标是部署SynBio工具，以改善B中的内源和异源酶生产经济性。licheniformis通过领先的最终用户进行开发。基因组递送技术将扩增/靶向基因到经验证的高水平/可预测表达的位置，克服与非靶向整合相关的问题。基于核酸酶的基因组编辑将a）解决通过系统生物学鉴定的胁迫条件下宿主代谢的产量妥协方面，以及B）利用原位蛋白质工程来改善内源酶功能，包括耐热性和操作条件下的最佳活性。这些领域的成功将降低成本，提高B生产的工业酶的多功能性和效率。地衣形的