ALTERATION OF THE ERYTHROCYTE MEMBRANE DURING MALARIA

疟疾寄生虫在其红细胞期会改变宿主的 薄膜。因为红细胞膜的改变很可能是 寄生虫生存所必需的，更好地了解 宿主和寄生虫之间的相互作用将为我们提供对小说的洞察 治疗方法。建议的长远目标 研究是为了描述和更好地理解相互作用 在寄生虫和红细胞膜之间的分子或 生化水平。尤其是寄生虫来源的蛋白质，但 与宿主红细胞膜相关，将被研究。一 该提案的具体目标之一是克隆和测序基因 啮齿动物的三种先前鉴定的酸性磷酸蛋白 与疟原虫细胞质表面相互作用的疟疾寄生虫 红细胞膜。93 kDa疟原虫gt11的克隆 Chabuadi酸性磷酸蛋白已经被分离出来，65和 46 kDa伯氏假单胞菌酸性磷酸蛋白的筛选 含合成寡核苷酸的重组DNA文库(基于 蛋白质的氨基酸序列)。酸性分子的序列 磷蛋白将提供关于它们的结构和它们的 与其他蛋白质的关联性。克隆的基因也将被用作 检测酸性磷酸蛋白在人体内分布的探针 疟疾物种，从而提供了一个应用于 人类疾病情况，并提供关于人类疾病演变的信息 宿主与寄生虫的相互作用。克隆基因的长期计划包括 大量生产用于生产的重组蛋白 功能研究。此外，现有的单抗 将被用来定位酸性磷酸蛋白在 免疫电子显微镜超微结构水平及作用机制的研究 将酸性磷酸蛋白从寄生虫运送到 红细胞膜将通过脉冲追逐实验进行检测。在……里面 综上所述，拟议的研究将提供有关 酸性物质的结构、功能和转运机制 磷蛋白。这些结果将有助于我们了解 宿主-寄生虫相互作用的分子生物学，并最终可能 导致新的治疗方法的发展。此外， 这样的研究将提供有关结构和功能的信息 大体上是膜。

The malarial parasite, during its erythrocytic stage, alters the host's membrane. Since the alterations of the erythrocyte membrane are probably necessary for parasite survival, a better understanding of the interactions between host and parasite will provide insight into novel therapeutic approaches. The long-term objectives of the proposed research are to characterize and better understand the interactions between the parasite and the erythrocyte membrane on a molecular or biochemical level. In particular, proteins of parasite origin, but associated with the host erythrocyte membrane, will be investigated. One of the specific aims of the proposal is to clone and sequence the genes of three previously characterized acidic phosphoproteins from rodent malarial parasites that interact with the cytoplasmic face of the erythrocyte membrane. A lambda gt11 clone of the 93 kDa Plasmodium chabuadi acidic phosphoprotein has already been isolated, and the 65 and 46 kDa P. berghei acidic phosphoproteins will be cloned by screening recombinant DNA libraries with synthetic oligonucleotides (based upon the amino acid sequences of the proteins) . The sequences of the acidic phosphoproteins will provide information about their structures and their relatedness to other proteins. The cloned genes will also be used as probes to examine the distribution of the acidic phosphoproteins in human malarial species, thus providing a framework for applications to the human disease situation and providing information about the evolution of host-parasite interactions. Long-term plans for the cloned genes include the production of large quantities of recombinant proteins to be used in functional studies. In addition, the available monoclonal antibodies will be used to localize the acidic phosphoproteins on the ultrastructural level using immuno-electron microscopy, and the mechanism of transport of the acidic phosphoproteins from the parasite to the erythrocyte membrane will be examined by pulse-chase experiments. In summary, the proposed studies will provide information concerning the structures, functions, and mechanism of transport of the acidic phosphoproteins. These results will contribute to our knowledge about the molecular biology of host-parasite interactions and may eventually lead to the development of novel therapeutic approaches. Furthermore, such studies will provide information about the structure and function of membranes in general.