FACTORS INFLUENCING SURVIVAL OF VASCULARIZED BONE GRAFTS

影响血管化骨移植存活的因素

• 批准号: 2078548
• 负责人: Joseph Michael Lane
• 金额: $ 15.3万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 1979
• 资助国家: 美国
• 起止时间: 1979-07-01 至 1995-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2078548
• 关键词: angiogenesis; biomaterial development /preparation; biomaterial evaluation; bone marrow; bone regeneration; bone transplantation; cell differentiation; ceramics; fluorescence microscopy; histochemistry /cytochemistry; histology; homologous transplantation; hydroxyapatites; implant; laboratory rabbit; microangiography; musculoskeletal circulation; orthopedics; osteoblasts; osteogenesis; scanning electron microscopy; tissue /cell culture; transforming growth factors; wound healing

The specific aims of this application are to continue the investigation of factors influencing the principles of vascularized bone grafts. Currently available vascularized bone grafts used to reconstruct massive segmental bone loss in compromised beds following wide resections of malignant bone tumors, traumatic bony defects, and infected non-unions have significant limitations resulting from structural confirmation of the graft, and donor site morbidity. The central goal of this proposal is to utilize biosynthetic components (hydroxyapatite ceramic mold), osteoinductive principles and osteoprogenitor cells coupled with a vascularized pedicle to produce "a molded vascularized osteogenesis". The second goal of this proposal is to isolate bone marrow stromal osteoprogenitor cells, determine the appropriate degree of differentiation that maximizes osteogenesis in vivo and expand this to defined populations ex vivo prior to utilization in molded osteogenesis. Studies have been designed to isolate the characterize osteoprogenitor cells from rabbit marrow in vitro and utilize diffusion chambers to determine the appropriate level of contact with matrix host cells and to determine the effects of demineralized bone matrix and of growth factors on heterotopic bone regeneration. This vascularized molded bone graft will be used as an island graft or a free transfer and the healing and material properties of these grafts will be examined and compared to conventional bone grafts.

此应用程序的具体目的是继续进行调查