BUNYAVIRUS/MOSQUITO INTERACTIONS

布尼亚病毒/蚊子相互作用

• 批准号: 2072633
• 负责人: KEVIN T SCHULTZ
• 金额: $ 14.88万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-04-01 至 1998-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2072633
• 关键词: Aedes; Bunyaviridae; disease /disorder model; disease vectors; epitope mapping; host organism interaction; laboratory mouse; nervous system infection; tissue /cell culture; virus diseases; virus infection mechanism; virus replication

There is specificity of California serogroup bunyaviruses for particular mosquitoes. Because of this vector specificity even when two human encephalitis viruses such as La Crosse and Jamestown Canyon exist in the same area, they maintain separate life cycles. The recently introduced non-native mosquito Aedes albopictus can be infected with both viruses. Moreover, this mosquito is colonizing many areas of the United States where both viruses are present. It seems reasonable to assume that this mosquito will become infected with both viruses and this co-infection will result in the generation of JC/LACV reassorted viruses. The long term goal of the proposed research is to understand the interaction of a bunyavirus with its mosquito vector. Specifically, the research aims are: 1) to determine the genetic basis for differences in insect transmission of lACV and JCV by the use of reassorted viruses 2) to determine the requirement of genome segments in infection, amplification and neuropathology in mammals 3) to identify epitopes on lACV G2 responsible for attachment of the virus to mosquito cells and determine the amino acid sequences involved and to examine other bunyaviruses for similar epitopes. Moreover, these reassortants may have a significant public health threat and examination of these viruses and their vector should allow us to assess this risk. Future studies may exploit this knowledge for the prevention of bunyavirus diseases using techniques such as transgenic mosquitoes.

加州血清群本扬病毒对特定的 蚊子 由于这种载体特异性，即使两个人 脑炎病毒，如拉克罗斯和詹姆斯敦峡谷存在于 在同一地区，它们保持着不同的生命周期。 最近推出的 非本地蚊子白纹伊蚊可以感染这两种病毒。 此外，这种蚊子正在美国的许多地区定居， 两种病毒都存在。 似乎有理由认为这种蚊子 会同时感染这两种病毒，这种合并感染会导致 JC/LACV重组病毒的产生。 的长期目标 拟议的研究是了解布尼亚病毒与其 蚊媒。 具体而言，研究目的是：1）确定 通过对IACV和JCV的昆虫传播差异的遗传基础的研究， 使用重组病毒2）确定基因组的需求 哺乳动物中的感染、扩增和神经病理学片段3）至 鉴定IACV G2上负责将病毒附着于 蚊子细胞，并确定所涉及的氨基酸序列， 检查其他布尼亚病毒的相似表位。 而且这些 食品可能对公众健康构成重大威胁， 这些病毒和它们的载体应该使我们能够评估这种风险。 未来 研究可以利用这些知识来预防布尼亚病毒 利用转基因蚊子等技术来预防疾病。