BMP 5 MEDIATION BONE MECHANICAL ADAPTATION AND REPAIR

这项研究的目的是检查骨形态发生的影响 骨骼生长过程中的蛋白-5(BMP-5)。了解在体内的作用 BMP-5的应用将使这些生长因子被考虑用于骨质疏松症 治疗和治疗。长骨功能适应与再生 将在短耳小鼠和它的对照小鼠身上进行检查 两项实验研究。此动物模型删除了 BMP-5基因。这两组实验的设计都是为了评估 以及短耳小鼠和年龄匹配小鼠的长期骨组织反应 控制。这些研究结合在一起，加深了我们对 骨形态发生蛋白在骨生长、适应和再生中的作用机制。 在第一组实验中，BMP-5在机械-骨形成过程中的作用 后肢功能适应将被研究 暂停和暂停，然后重新装填。卸货过程中 后肢悬吊将使用体内应变计进行量化。 将检查股骨的结构、几何和材料特性 遗传效应对适应性反应的影响。根据实验数据， 长骨生长发育的解析模型 将进行细化和扩展，以包括非机械效果。 第二组实验将检测BMP-5在骨折中的作用 治愈。标准的股骨干闭合性骨折将是 已创建。短期的组织反应将在组织学上量化 在骨折后的前两周，和长期的生物力学 愈合的股骨的完整性将在前五个阶段进行评估 骨折后几周。

The goal of this research is to examine the effects of bone morphogenetic protein-5 (BMP-5) during skeletal growth. Understanding the in vivo role of BMP-5 will allow these growth factors to be considered for osteoporosis treatment and therapy. Long bone functional adaptation and regeneration will be examined in the short ear mouse and its control litter mates in a pair of experimental studies. This animal model has a deletion for the BMP-5 gene. Both sets of experiments are designed to evaluate the short and long-term bone tissue responses in the short ear mouse and age-matched controls. These studies combine to enhance our understanding of the mechanisms of action of BMPs in bone growth, adaptation and regeneration. In the first set of experiments, the role of BMP-5 in mechanically- regulated functional adaptation will be studied following hindlimb suspension and suspension followed by reloading. Unloading during hindlimb suspension will be quantified using in vivo strain gages. Femoral structural, geometric and material properties will be examined for genetic effects on the adaptational response. With the experimental data, previously-developed analytical models of long bone growth and development will be refined and expanded to include non-mechanical effects. The second set of experiments will examine the role of BMP-5 in fracture healing. Standard closed fractures of the femoral diaphysis will be created. Short-term tissue response will be quantified histologically during the first two weeks post-fracture, and long-term biomechanical integrity of the healing femur will be evaluated during the first five weeks following fracture.