CELL ADHESION IN TISSUE MORPHOGENESIS

组织形态发生中的细胞粘附

• 批准号: 2093228
• 负责人: WILLIAM G. CARTER
• 金额: $ 28.26万
• 依托单位: FRED HUTCHINSON CANCER RESEARCH CENTER
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-12-01 至 1997-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2093228
• 关键词: basement membrane; cell adhesion; cell differentiation; cellular polarity; collagen; cytokine; epithelium; extracellular matrix; extracellular matrix proteins; histogenesis; human tissue; integrins; keratinocyte; laboratory mouse; laboratory rabbit; molecular cloning; monoclonal antibody; protein sequence; receptor binding; receptor expression

Our long term goals are to determine the receptors and mechanisms involved in cell interactions with the extracellular matrix (ECM) and other cells and how these recaptors function cooperatively in differentiation and morphogenic processes. We have defined three structurally and functionally related ECM receptors (ECMRs), II, VI and I, of 140 kDa each that mediate mesenchymal cell adhesion to collagen, fibronectin and collagen/fibronectin/laminin, respectively, We have also defined a fourth ECMR of 90 kDa, that binds to collagen, termed collagen receptor III (CRIII), that is expressed in most nucleated cells. CRIII interacts with a proteoglycan (GAG200) and is homologous to the Hermes-1 and Mel 14 antigens, mediators of lymphocyteendothelial cell adhesion. Thus, CRIII may mediate cell-cell adhesion in many cell populations as well as interact with the ECM. The ECMR are also expressed by epithelial cells and often ECM ligand suggesting additional possible functions for the ECMRs. This proposal will utilize biochemical and immunological techniques to evaluate; 1. THE FUNCTIONAL ROLE OF CRIII IN MEDIATING INTERCELLULAR ADHESION IN MULTIPLE CELL POPULATIONS. (A) Development of assays to examine the role of CRIII in cell-cell adhesion. (B) Identification of a ligand, possible GAG200 or collagen, for CRIII involved in cell-cell adhesion. II.THE FUNCTIONAL ROLE OF ECMRS IN LOCALIZING EPITHELIAL AND OTHER CELLS TO TISSUE DOMAINS (A) Localization of ECMRs in normal and malignant tissue in relation to ECM components. (B) Development of assays to evaluate the role of ECM-ECMB interactions in epithelium. (C) The role of ECM-ECMR interactions in expression of epithelial cell characteristics (polarity, migration, differentiation).

我们的长期目标是确定相关的受体和机制 细胞与细胞外基质（ECM）和其他细胞的相互作用 以及这些受体如何在分化中协同作用， 形态发生过程我们在结构和功能上定义了三个 相关ECM受体（ECMR），II、VI和I，各自为140 kDa，其介导 间充质细胞与胶原蛋白、纤连蛋白和 胶原蛋白/纤连蛋白/层粘连蛋白，我们还定义了第四个 ECMR为90 kDa，与胶原蛋白结合，称为胶原蛋白受体III （CRIII），其在大多数有核细胞中表达。中区填海第三期工程与 蛋白聚糖（GAG 200），并且与爱马仕-1和Mel 14同源 抗原，淋巴细胞内皮细胞粘附介质。因此，中区填海第三期工程可 在许多细胞群中介导细胞-细胞粘附， 与ECM。ECMR也由上皮细胞表达，并且通常由ECM表达。 配体提示ECMR的其他可能功能。这 建议将利用生物化学和免疫学技术进行评估; 1. CRIII在介导细胞间粘附中的作用 多细胞群体。(A)开发检测方法，以检查 CRIII在细胞-细胞粘附中的作用。(B)可能识别配体 GAG 200或胶原蛋白，用于参与细胞-细胞粘附的CRIII。II.THE ECMRS在上皮细胞和其他细胞定位于组织中的功能作用 结构域（A）ECMR在正常和恶性肿瘤组织中的定位 与ECM组件的关系。(B)开发检测方法，以评估 ECM-ECMB的相互作用。(C)ECM-ECMR的作用 上皮细胞特征表达中的相互作用（极性， 迁移、分化）。