ANTIPROLIFERATIVE EFFECT OF WILD-TYPE P53

编码核磷蛋白p53的基因是一个常见的靶点， 在各种各样的人类肿瘤中的突变。 野生型p53蛋白 正常p53基因编码的p53基因表现出有效的生长抑制作用， 该活性在蛋白质的突变形式中不存在。 最近 研究表明，p53蛋白可以发挥积极和消极的， 对基因表达的影响 野生型p53蛋白的能力， 调节基因表达与其生长抑制有关 活动 我们之前已经证明过表达野生型- 型p53蛋白的表达下调， 编码增殖细胞核的内源性生长调节基因 抗原（PNCA），p53反应元件的B-鉴定和 与p53协同作用的核蛋白组分的表征 在调节这些基因中的两个的表达中; PCNA和DNA 聚合酶α 从这项研究中获得的信息将增加 了解P53介导的基因调控机制 表情

The gene encoding the nuclear phosphoprotein p53 is a frequent target of mutation in a wide variety of human tumors. The wild-type p53 protein encoded for the normal p53 gene exhibits a potent growth-suppressing activity which is absent from mutant forms of the protein. Recent studies have shown that p53 proteins can exert both positive and negative effects on gene expression. The ability of the wild-type p53 protein to modulate gene expression has been linked to its growth-suppressing activity. We have previously demonstrated that overexpression of wild- type p53 protein is correlated with down-regulation in the expression of the endogenous growth-regulated genes encoding proliferating cell nuclear antigen (PNCA), B-identification of p53-responsive elements and the characterization of nuclear protein components that collaborate with p53 in modulating the expression of two of these genes; PCNA an DNA polymerase alpha. Information obtained from this study will increase out understanding of the mechanisms of P53-mediated regulation of gene expression.