TRANSCRIPTIONAL REPRESSION BY FUR REPRESSOR PROTEIN

细胞Fe（II）库的严格调节对于防止细胞内Fe（II）库的形成至关重要。 有害副产品的积累。 铁吸收调节（Fur） 来自大肠杆菌的阻遏蛋白是铁稳态的一部分， 抑制Fe（II）清除转录的系统和功能 铁丰富的条件下的机械。 有了这个支持 奖学金，这个系统如何运作的基本知识可能是 收集，然后可以应用于铁的调节系统， 其他金属离子在高等生物体中。 拟议的工作将解决这两个问题 Fur对Fe（II）的选择性和灵敏度 金属离子 一组光谱学方法将被用来识别 金属离子的配位环境。 dna结合 将测试络合物的性质，并将Fe（II）-Fur 将评估充当高响应金属传感器的能力。 的 蛋白质/DNA接触将被映射和识别。 最后，角色 铁（II）在转录抑制机制将是 已解决。

Tight regulation of the cellular Fe(II) pool is imperative to prevent the accumulation of harmful byproducts. The Ferric Uptake Regulation (Fur) repressor protein from Escherichia coli is part of the iron homeostatic system and functions to repress transcription of Fe(II)-scavenging machinery under conditions of iron abundance. With the support of this fellowship, fundamental knowledge of how this system functions may be gathered which can then be applied to regulatory systems for iron and other metal ions in higher organisms. Proposed work will address both the selectivity and the sensitivity of Fur for Fe(II) relative to other metal ions. A battery of spectroscopic methods will be used to identify the coordination environments of the metal ions. The DNA-binding properties of the complex will be tested, and the potential of Fe(II)-Fur to act as a highly responsive metal sensor will be assessed. The protein/DNA contacts will be mapped and identified. Finally, the role of Fe(II) in the mechanism of transcriptional repression will be addressed.