PICOGRAM NON-SPECIFIC DNA DETECTION SYSTEM

描述（改编自申请人摘要）：基于初步 结果，构建了一个通用的系统，用于检测DNA在 皮克（1 × 10（-12））级是可能的。该系统将在 大多数水溶液中的DNA序列无关。 作为 建议，它不需要复杂的仪器，应该 能够检测样本中的DNA，试剂成本约为50美分， 特别地，该系统将能够检测DNA中的DNA。 存在大量的蛋白质，碳水化合物，和小 分子。 该系统的组件可商购获得。 关键在于 商业上可行的系统是在以下条件下对测定技术进行验证： 潜在客户可能感兴趣和受益的条件， 从事实验室研究和药品生产的人。一个简单 定量复杂混合物中DNA浓度的可靠方法 是必要的。 如果不纯化DNA， available.

DESCRIPTION (Adapted from applicant's abstract): Based on preliminary results, construction of a general system for detection of DNA at the picogram (1 x 10(-12)) level may be possible. The system would work in most aqueous solutions without regard to the sequence of the DNA. As proposed, it does not require sophisticated instrumentation and should be able to detect DNA in samples for a reagent cost of about 50 cents per assay.In particular, the system will be able to detect DNA in the presence of large amounts of proteins, carbohydrates, and small molecules. Components of the system are commercially available. The key to making a commercially viable system is validation of the assay technique under conditions likely to be of interest and benefit to potential customers, people doing laboratory research and pharmaceutical production. A simple and reliable method of quantitating DNA concentration in complex mixtures is needed. without purifying DNA, no sequence independent method is available.