PICOGRAM NON-SPECIFIC DNA DETECTION SYSTEM

皮克非特异性 DNA 检测系统

• 批准号: 2189477
• 负责人: GORDON B COLLIER
• 金额: $ 7.91万
• 依托单位: TREVIGEN, INC.
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-05-01 至 1994-10-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2189477
• 关键词: DNA; alkaline phosphatase; colorimetry; cytosine analog; enzyme linked immunosorbent assay; hydroxylamine; immunofluorescence technique; luminescence; method development; monoclonal antibody; sulfates; sulfation; western blottings

DESCRIPTION (Adapted from applicant's abstract): Based on preliminary results, construction of a general system for detection of DNA at the picogram (1 x 10(-12)) level may be possible. The system would work in most aqueous solutions without regard to the sequence of the DNA. As proposed, it does not require sophisticated instrumentation and should be able to detect DNA in samples for a reagent cost of about 50 cents per assay.In particular, the system will be able to detect DNA in the presence of large amounts of proteins, carbohydrates, and small molecules. Components of the system are commercially available. The key to making a commercially viable system is validation of the assay technique under conditions likely to be of interest and benefit to potential customers, people doing laboratory research and pharmaceutical production. A simple and reliable method of quantitating DNA concentration in complex mixtures is needed. without purifying DNA, no sequence independent method is available.

描述（改编自申请人的摘要）：基于初步 结果，构建了用于检测 DNA 的通用系统 皮克 (1 x 10(-12)) 级别是可能的。该系统将工作在 大多数水溶液，而不考虑 DNA 的序列。 作为 建议，它不需要复杂的仪器，并且应该 能够检测样本中的 DNA，试剂成本约为 50 美分 特别是，该系统将能够检测样品中的 DNA 存在大量的蛋白质、碳水化合物和小分子物质 分子。 该系统的组件可在市场上买到。 制作的关键 商业上可行的系统是对测定技术的验证 潜在客户可能感兴趣并受益的条件， 从事实验室研究和药品生产的人员。一个简单的 复杂混合物中 DNA 浓度定量的可靠方法 是需要的。 在不纯化 DNA 的情况下，没有任何独立于序列的方法 可用的。