超灵敏化学发光酶联检测-猫眼课题宝

超灵敏化学发光酶联检测

批准号：

2147543

负责人：

Hashem Akhavan-Tafti

金额：

$7.45万

依托单位：

LUMIGEN, INC.

依托单位国家：

美国

项目类别：

财政年份：

1994

资助国家：

美国

项目状态：

已结题

起止时间：

1994-03-01 至 1994-09-30

来源：

https://reporter.nih.gov/project-details/2147543

关键词：

AIDS /HIV test HIV envelope protein gp120 alkaline phosphatase chemical synthesis chloride channels cystic fibrosis enzyme substrate human genetic material tag inborn metabolism disorder diagnosis luminescence method development nucleic acid probes nucleic acid sequence oligonucleotides rapid diagnosis southern blotting western blottings

中文摘要

翻译

拟议研究的目的是证明 21个氨基酸多肽的超灵敏化学发光分析免疫印迹和囊性纤维化跨膜技术检测HIV gp120蛋白 Southern blotting检测到调节基因。我们已经开发出一种专有的一种增敏的酶标记化学发光检测系统以固相检测格式。共同使用的专有衬底标记酶碱性磷酸酶和促进剂提供了极好的检测的特异性、敏感性和速度。我们将开发一套用于检测蛋白质可行性的化学发光试剂以及上述检测中的核酸。拟议的研究应该证明新一代化学发光试剂用于化学发光的可行性酶检测及化学发光检测新方法具有生物医学意义的分析物。最初的商业应用将针对HIV gp120和囊性纤维化跨膜调节基因，它将在第一阶段开发。

英文摘要

The objective of the proposed research is to prove feasibility of ultrasensitive chemiluminescent assays for the 21 amino acid polypeptide from HIV gp 120 by Western blotting and cystic fibrosis transmembrane regulator gene by Southern blotting. We have developed a proprietary enzyme-label chemiluminescent detection system with enhanced sensitivity in solid-phase detection formats. Proprietary substrates for the common marker enzyme alkaline phosphatase and enhancers provide excellent specificity, sensitivity and speed of detection. We will develop sets of chemiluminescent reagents to test feasibility of detection of proteins and nucleic acids in the above assays. The proposed research should prove the feasibility of a new generation of chemiluminescent reagents for enzyme detection and a novel method of chemiluminescent detection of analytes of biomedical significance. The initial commercial application will be for HIV gp 120 and cystic fibrosis transmembrane regulator gene, which will be developed in Phase Il.

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