超灵敏化学发光酶联检测-猫眼课题宝

超灵敏化学发光酶联检测

批准号：

2147543

负责人：

Hashem Akhavan-Tafti

金额：

$7.45万

依托单位：

LUMIGEN, INC.

依托单位国家：

美国

项目类别：

财政年份：

1994

资助国家：

美国

项目状态：

已结题

起止时间：

1994-03-01 至 1994-09-30

来源：

https://reporter.nih.gov/project-details/2147543

关键词：

AIDS /HIV test HIV envelope protein gp120 alkaline phosphatase chemical synthesis chloride channels cystic fibrosis enzyme substrate human genetic material tag inborn metabolism disorder diagnosis luminescence method development nucleic acid probes nucleic acid sequence oligonucleotides rapid diagnosis southern blotting western blottings

中文摘要

翻译

本研究的目的是证明以下方法的可行性 21个氨基酸多肽的超灵敏荧光分析从HIV gp120蛋白质印迹和囊性纤维化跨膜 Southern杂交检测调控基因。我们开发了一种专有的具有增强灵敏度的酶标记荧光检测系统以固相检测的形式。专利基板的共同标记酶碱性磷酸酶和增强剂提供了极好的特异性、灵敏度和检测速度。我们将开发一套用于测试蛋白质检测可行性的荧光试剂和核酸。这项研究应该证明新一代荧光试剂用于酶检测和荧光检测的新方法具有生物医学意义的分析物。最初的商业应用将为HIV gp120和囊性纤维化跨膜调节基因，将在第二阶段开发。

英文摘要

The objective of the proposed research is to prove feasibility of ultrasensitive chemiluminescent assays for the 21 amino acid polypeptide from HIV gp 120 by Western blotting and cystic fibrosis transmembrane regulator gene by Southern blotting. We have developed a proprietary enzyme-label chemiluminescent detection system with enhanced sensitivity in solid-phase detection formats. Proprietary substrates for the common marker enzyme alkaline phosphatase and enhancers provide excellent specificity, sensitivity and speed of detection. We will develop sets of chemiluminescent reagents to test feasibility of detection of proteins and nucleic acids in the above assays. The proposed research should prove the feasibility of a new generation of chemiluminescent reagents for enzyme detection and a novel method of chemiluminescent detection of analytes of biomedical significance. The initial commercial application will be for HIV gp 120 and cystic fibrosis transmembrane regulator gene, which will be developed in Phase Il.

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