LARYNGEAL DEVELOPMENT AND NERVE REGENERATION

急性喉返神经（RLN）损伤的患者不会 不仅声带运动受损的功能后果，而且 减少沟通的心理影响。患者 单侧喉返神经损伤可导致失音和误吸， 而双侧喉返神经损伤阻塞气道，因此需要 气管切开术这些条件往往是不适当的直接结果 喉肌的神经再支配的轴突是错误的，在 再生的过程。神经再支配的过程受到 促进细胞粘附的表面分子。理想情况下，该过程应 模拟正常的发育过程，其中轴突-轴突和轴突- 肌肉连接正确。当正确的连接失败时， 发展，出现残余喉无力和功能障碍。 本研究的目的是明确运动单位的细胞变化 负责观察喉返神经再支配模式的组织 大鼠喉肌组织。拟议的研究继续了以前的工作， 提示喉神经再支配的模式受 内收肌运动神经元优先接触喉肌 疑核为了进一步评估喉的过程， 在大鼠模型中，再生的运动神经元将 使用逆行标记根据其原始功能进行分类 技术.细胞数量和体细胞大小将与代谢相关。 与神经损伤相关的改变，使用组织化学分析， 琥珀酸脱氢酶和细胞色素氧化酶。免疫化学 技术将被用来评估细胞粘附的作用， 神经再生过程中的分子， 表达与喉运动神经元和肌肉功能有关。 将比较修复中细胞粘附分子表达的模式 发生在正常发育的中枢和外周的事件 神经系统 这是加拿大国际开发署的申请是基于拟议的研究和先进的 发育神经生物学、免疫细胞化学、电子 显微镜和生物统计学。基本培训旨在培养 应用研究计划侧重于喉的发展和机制 RLN的再生。

The patient with acute recurrent laryngeal nerve (RLN) injury suffers not only the functional consequences of impaired vocal fold motion, but also the psychological impact of reduced communication. Patients with unilateral RLN injury are variably disabled by aphonia and aspiration, while bilateral RLN injuries obstruct the airway, thereby necessitating tracheotomy. These conditions are often the direct result of inappropriate reinnervation of laryngeal muscles by axons that are misdirected in the course of regeneration. The process of reinnervation is influenced by surface molecules that promote cell adhesion. Ideally, this process should simulate the normal developmental process wherein axon-axon and axon- muscle connections are made correctly. When proper connections fail to develop, residual laryngeal weakness and impairment of function occur. The purpose of this study is to define the cellular changes in motor unit organization responsible for observed patterns of RLN reinnervation of the rat laryngeal musculature. The proposed study continues earlier work which suggests that patterns of laryngeal reinnervation are subject to preferential contact of the laryngeal muscles by adductor motoneurons of the nucleus ambiguus. To further evaluate the process of laryngeal reinnervation in the rat model, regenerating motoneurons will be classified according to their original function using retrograde labeling techniques. Cell number and somatic size will be correlated with metabolic alterations associated with nerve injury using histochemical assays for succinic dehydrogenase and cytochrome oxidase. Immunocytochemistry techniques will be employed to evaluate the role of cell adhesion molecules in the process of reinnervation, specifically addressing expression with respect to laryngeal motoneuron and muscle function. Patterns of cell adhesion molecule expression in repair will be compared to the events occurring in the normal developing central and peripheral nervous system. This CIDA application is based on the proposed studies and advanced training in developmental neurobiology, immunocytochemistry, electron microscopy and biostatistics. The basic training is intended to develop an applied research program focused on laryngeal development and mechanisms of RLN regeneration.