AMALGAM-INDUCED CHANGES IN ANTIBIOTIC RESISTANT FLORA

汞合金引起的抗生素耐药菌群变化

• 批准号: 3425943
• 负责人: Anne O'Neill Summers
• 金额: $ 5.1万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-08-01 至 1995-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3425943
• 关键词: Bacteroides gingivalis; Enterobacteriaceae; R factors; antibiotics; dental amalgam; drug resistance; feces analysis; genetic strain; human subject; mercury; microorganism culture; microorganism growth

The most important effects of dental restorative materials are considered to be those which directly impinge on the physiology of the human host. However, our recent laboratory studies have demonstrated that installation of amalgam fillings in monkeys results in a rapid proliferation of Hg and antibiotic resistant oral and intestinal bacteria. The possibility that this amalgam-provoked increase in bacterial resistance might also occur in humans is suggested by epidemiological data indicating a strong correlation between the occurrences of Hg resistance and of multiple antibiotic resistances in the intestinal flora of humans, even those known not to have recently consumed an antibiotic. Unfortunately, data specifically correlating the proliferation of resistant flora to amalgam status of individual humans are presently lacking. The current public health concern with the increasing prevalence of antibiotic resistant pathogens makes it very important to determine whether the routine exposure to Hg arising from dental amalgams can stimulate the proliferation of multiply antibiotic resistant bacteria in the human host. Thus, we propose to quantify the changes in Hg and antibiotic resistances in the gingival and intestinal bacteria of individual human subjects on the occasion of their receiving their first dental amalgam restorations. We will test the hypothesis, arising from our animal studies, that installation of amalgam fillings in a naive subject (i.e. one who has never had any amalgam fillings), leads to a proliferation of rare pre- existing Hg and antibiotic resistant bacterial strains in both the gingival and intestinal flora. It is important to determine whether human flora responds in the same way as the monkey flora because, such an enrichment of antibiotic resistance in human normal flora in response to the selective pressure of Hg released from dental amalgam would constitute a novel and previously unrecognized risk factor in the widespread use of amalgam dental restorations.

牙科修复材料的最重要的影响被认为是 是那些直接影响人类宿主生理的病毒。 然而，我们最近的实验室研究表明， 汞合金填充物在猴子体内的扩散导致汞的迅速扩散， 耐抗生素的口腔和肠道细菌。 的可能性 这种混合物引起的细菌耐药性的增加也可能发生在 流行病学数据表明， 抗汞性和多重耐汞性之间的相关性 人类肠道植物群中的抗生素耐药性，即使是那些已知的 最近没有服用过抗生素 不幸的是，数据 具体地将抗性植物群的增殖与汞合金相关联 人类个体的状态目前是缺乏的。 当前公共 随着抗生素耐药性的日益普遍， 病原体使得确定是否常规 接触牙科汞合金产生的汞可刺激 人类宿主中多重抗生素抗性细菌的增殖。 因此，我们建议量化汞和抗生素耐药性的变化 在牙龈和肠道细菌的个体人类受试者 在他们接受第一次牙科汞合金矫正的时候。 我们将检验从动物研究中得出的假设， 在幼稚受试者体内安装汞合金填充物（即 从未使用过任何汞合金填充物），导致罕见的预- 现有的汞和抗生素耐药菌株， 牙龈和肠道植物群。重要的是要确定人类是否 植物群的反应和猴子的植物群一样， 人类正常植物群中抗生素耐药性的富集 从牙科汞合金中释放汞的选择性压力将构成 一种新的和以前未被认识的危险因素，在广泛使用的 汞合金牙科矫正