MODEL DIURNAL CIRCADIAN MAMMAL

所描述的研究的主要目的是确定 奥特登·德古斯是一种南美昼夜活动的杂交类啮齿类动物 适合的昼夜节律动物模型最终用于 人类昼夜节律紊乱的研究及与一般相关的研究 昼夜哺乳动物昼夜节律的神经生物学控制。 以前开发昼夜动物模型的尝试受到以下限制 很难获得足够的动物，昼夜节律 足够嘈杂，以至于排除了对形式属性的研究 昼夜节律系统，实验室中的行为不符合 实地报告的数据或方法仅限于收集 并对数据进行分析。到目前为止，很少有研究人员试图找到 适合日间活动的物种以广泛描述形式 昼夜节律特征。 开发一种昼夜啮齿动物模型将允许更具成本效益的 而不是使用人类受试者或灵长类动物来开发治疗方法 人类疾病，如跨子午线喷气式飞机引起的问题 旅行、轮班工作、疾病和因创伤问题而住院。 这些情况会导致精神警觉性的恶化，睡眠 破裂、溃疡，在最严重的情况下，还有抑郁症。物美价廉 昼夜啮齿动物模型也将允许广泛的比较生理学 和神经生物学研究。 因此，该项目的主要目标是确定 Degu的形式昼夜节律属性来确定它是否可以证明 成为昼夜活动的模范物种。目前可用于周日的数据 物种表明这些物种的昼夜节律功能可能不同 与夜间活动物种有很大不同。 要确定正式的昼夜节律属性，请使用以下参数 将在成年男性和女性中进行检查：1)稳定性和 携带的昼夜活动节律的精确性。2)再夹带 对明暗环境中不同大小相移的响应(时间和形式) (LD)循环。3)自由奔跑节律的稳定性及其依赖性 关于恒定的照度(阿肖夫法则)。4)发展 使用1小时光脉冲的动物的相响应曲线(PRC) 在持续的黑暗中运行(DD)。5)夹带的限度(各种 LD比率)。6)Ld幅值在夹带中的相对重要性。7) 活度和温度节律夹带与游离态的比较 奔跑的节奏。8)社交线索起到非光波作用的能力 夹带剂。

The primary purpose of the research described is to determine whether the Octodon degus, a South American, diurnal, hystricimorph rodent is a suitable animal model of diurnal circadian rhythms to eventually use in studies of human circadian disorders and research related to the general neurobiological control of circadian rhythms in diurnal mammals. Previous attempts to develop a diurnal animal model have been limited by difficulty obtaining sufficient animals, circadian rhythms were sufficiently noisy so as to exclude studies of the formal properties of the circadian system, behaviors in the laboratory did not agree with those reported in the field, or methodologies were limited for gathering and analyzing data. To date, few researchers have attempted to find a suitable day-active species for extensive description of the formal circadian properties. Development of a diurnal rodent model will allow a more cost-effective means than using human subjects or primates to develop treatments for human disorders such as the problems that result from transmeridian jet travel, shiftwork, illness and hospitalization for traumatic problems. These situations cause deterioration of mental alertness, sleep disruption, ulcers and, in the most severe cases, depression. A good diurnal rodent model will also allow extensive comparative physiological and neurobiological studies. Thus, the main objective of this project is the determination of the formal circadian properties of the degu to determine whether it may prove to be a model diurnal species. The data currently available for diurnal species indicate that circadian function in such species may differ substantially from that of nocturnal species. To determine the formal circadian properties, the following parameters will be examined in adult male and female degus: 1) The stability and precision of entrained circadian activity rhythms. 2) The reentrainment response (time and form) to various size phase-shifts in the light-dark (LD) cycle. 3) Stability of free-running rhythms and their dependence on constant levels of illumination (Aschoff's Rule). 4) Development of phase response curves (PRC's) using 1 hr light pulses for animals free- running in constant darkness (DD). 5) The limits of entrainment (various LD ratios). 6) Relative importance of LD amplitude in entrainment. 7) Comparison of activity and temperature rhythm entrainment and free- running rhythms. 8) Ability of social cues to act as a non-photic entraining agent.