CELLULAR AND MOLECULAR MECHANISMS OF TASTE TRANSDUCTION

味觉传导的细胞和分子机制

• 批准号: 2126886
• 负责人: JOHN H TEETER
• 金额: $ 16.07万
• 依托单位: MONELL CHEMICAL SENSES CENTER
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-12-01 至 1995-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2126886
• 关键词: alternatives to animals in research; arginine; biological models; biological signal transduction; biophysics; calcium channel; calcium flux; calcium indicator; catfish; chemoreceptors; glutamate receptor; glutamates; laboratory mouse; lipid bilayer membrane; membrane potentials; membrane reconstitution /synthesis; microscopy; taste; video microscopy; video recording system; voltage /patch clamp; voltage gated channel

DESCRIPTION: (From applicant's Abstract): The major goal of the proposed research is to define,for amino acid taste reception, the molecular mechanisms that link stimulus-receptor interaction with subsequent ionic events in the receptor cells. Research will focus on the study of taste transduction in two model systems: catfish (L-Arg) and mice (MSG). Voltage-dependent and stimulus-regulated changes in membrane conductance will be examined in isolated receptor cells using whole-cell recording techniques and stimulus-induced changes in intracellular calcium levels will be monitored in real time using fura-2 and video microscopy. Single-channel properties will be studied in excised patches and planar bilayers into which taste membrane fragments have been incorporated. L-Arg and MSG receptors, that the investigator hopes will be cloned by another investigator, will be functionally expressed in heterologous systems and compared with native receptors in terms of specificity, sensitivity, ion selectivity, channel kinetics and pharmacology. It is hoped that these experiments will provide a comprehensive description of amino acid taste signal transduction in the catfish model, as well as the basis for similar studies in mammalian taste cells.

描述：(摘自申请者摘要)： 拟议的研究是为了定义，对于氨基酸口味接收， 将刺激-感受器相互作用与 随后受体细胞中的离子事件。研究将集中于 关于两种模型系统的味觉转导研究 (L-Arg)和小鼠(味精)。电压依赖和刺激调节 膜电导的变化将在分离的受体中进行检测 使用全细胞记录技术的细胞和刺激诱导的变化 在细胞内钙水平将被实时监测使用 Fura-2和视频显微镜。将研究单通道属性 在切除的斑块和进入味觉膜的平面双层中 碎片已被合并。L-精氨酸和味精受体，即 调查员希望被另一名调查员克隆，将是 在异源系统中的功能表达及其与 天然受体的特异性、敏感性、离子 选择性、通道动力学和药理学。希望这些都是 实验将提供对氨基酸的全面描述 味觉信号转导在鲶鱼模型中的作用以及基础 在哺乳动物味觉细胞中进行类似的研究。