CELLULAR AND MOLECULAR MECHANISMS OF TASTE TRANSDUCTION

产品说明： (From申请人摘要）： 建议的研究是定义，对于氨基酸的味觉接受， 将刺激-受体相互作用与 受体细胞中随后的离子事件。 研究将集中 味觉传导的研究 在两个模型中 系统：鲶鱼 （L-精氨酸） 小鼠（MSG）。电压依赖和刺激调节 将在分离的受体中检测膜电导的变化。 使用全细胞记录技术和刺激诱导的变化 细胞内钙水平将被监测在真实的时间使用 Fura-2和视频显微镜。 将研究单通道特性 在切除的贴片和平面双层中，味膜 片段被合并。 L-Arg和MSG受体， 研究人员希望被另一名研究人员克隆， 在异源系统中功能性表达，并与 就特异性、敏感性、离子 选择性、通道动力学和药理学。希望这些 实验将提供氨基酸的全面描述 味觉信号转导的鲶鱼模型，以及基础 用于哺乳动物味觉细胞的类似研究。

DESCRIPTION: (From applicant's Abstract): The major goal of the proposed research is to define,for amino acid taste reception, the molecular mechanisms that link stimulus-receptor interaction with subsequent ionic events in the receptor cells. Research will focus on the study of taste transduction in two model systems: catfish (L-Arg) and mice (MSG). Voltage-dependent and stimulus-regulated changes in membrane conductance will be examined in isolated receptor cells using whole-cell recording techniques and stimulus-induced changes in intracellular calcium levels will be monitored in real time using fura-2 and video microscopy. Single-channel properties will be studied in excised patches and planar bilayers into which taste membrane fragments have been incorporated. L-Arg and MSG receptors, that the investigator hopes will be cloned by another investigator, will be functionally expressed in heterologous systems and compared with native receptors in terms of specificity, sensitivity, ion selectivity, channel kinetics and pharmacology. It is hoped that these experiments will provide a comprehensive description of amino acid taste signal transduction in the catfish model, as well as the basis for similar studies in mammalian taste cells.