AGE RELATED RESPONSE OF SALIVARY GLANDS TO ISOPROTERENOL
唾液腺对异丙肾上腺素的年龄相关反应
基本信息
- 批准号:2129978
- 负责人:
- 金额:$ 22.79万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1987
- 资助国家:美国
- 起止时间:1987-05-01 至 1997-04-30
- 项目状态:已结题
- 来源:
- 关键词:DNA binding protein DNA footprinting animal age group chloramphenicol acetyltransferase cysteine developmental genetics gel mobility shift assay gene expression genetic regulation genetic regulatory element hormone regulation /control mechanism hormone therapy isoproterenol laboratory rat male castration messenger RNA molecular cloning neurotransmitters nucleic acid sequence oligonucleotides protease inhibitor protein biosynthesis protein purification protein sequence reporter genes saliva submandibular gland tissue /cell culture transfection
项目摘要
Salivary glands produce secretions that bathe and protect the oral cavity.
Cystatin S, a naturally occurring cysteine proteinase inhibitor secreted by
submandibular glands, is believed to play a major protective role. Until
recently, nothing was known about the regulation of cystatin, S gene
expression despite the role these proteins play in oral pathophysiology.
Our long-range goal is to determine the mechanisms regulating cystatin
genes. The goal of the proposed research is to identify the functional
regulatory elements of the rat cystatin S gene, highly homologous to human
cystatin S genes, and the DNA binding proteins that modulate their
function. The Specific aims are to: 1) examine the hormonal, neuronal, and
developmental regulation of the rat cystatin S gene, 2) determine the
functional cis-acting DNA regulatory elements in the cystatin S gene, and
3) identify, isolate and characterize the trans-acting DNA binding proteins
regulating the rat cystatin S gene and the genes encoding them. We will
examine cystatin S mRNA levels in the submandibular glands of postnatally
developing males, of male and female rats after castration and hormone
replacement, and in glands whose innervation was blocked. Transfection of
segments of the 5' region of the cystatin S gene in constructs containing
the CAT reporter gene into appropriate recipient cells will allow us to
determine which of the potential regulatory sequences we have identified
are functional. Gel shift and DNase I protection assays will permit us to
identify the DNA binding proteins regulating this gene. Purifying these
proteins, obtaining their amino acid sequence and synthesizing
oligonucleotides corresponding to the amino acid sequence and generating
antibodies to these DNA binding proteins will make it possible to clone and
characterize the genes encoding these regulatory proteins. Knowledge of
how expression of cystatin genes is regulated may lead to clinical
treatments for patients who are orally compromised because of periodontal
disease, Sjogren's syndrome, and HIV disease.
唾液腺产生的分泌物可以沐浴和保护口腔。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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PHYLLIS Ann SHAW其他文献
PHYLLIS Ann SHAW的其他文献
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{{ truncateString('PHYLLIS Ann SHAW', 18)}}的其他基金
Controlled expression of a cystatin C-peptide as therapy for Alzheimer's disease
半胱氨酸蛋白酶抑制剂 C 肽的受控表达作为阿尔茨海默病的治疗方法
- 批准号:
8190713 - 财政年份:2011
- 资助金额:
$ 22.79万 - 项目类别:
Controlled expression of a cystatin C-peptide as therapy for Alzheimer's disease
半胱氨酸蛋白酶抑制剂 C 肽的受控表达作为阿尔茨海默病的治疗方法
- 批准号:
8323235 - 财政年份:2011
- 资助金额:
$ 22.79万 - 项目类别:
RIBOZYME MEDIATED KNOCKOUT OF THE CYSTATIN S GENE
核酶介导的胱抑素 S 基因敲除
- 批准号:
2132565 - 财政年份:1995
- 资助金额:
$ 22.79万 - 项目类别:
RIBOZYME MEDIATED KNOCKOUT OF THE CYSTATIN S GENE
核酶介导的胱抑素 S 基因敲除
- 批准号:
2132566 - 财政年份:1995
- 资助金额:
$ 22.79万 - 项目类别:
AGE-RELATED RESPONSE OF SALIVARY GLANDS TO ISOPROTERENOL
唾液腺对异丙肾上腺素的年龄相关反应
- 批准号:
3221952 - 财政年份:1987
- 资助金额:
$ 22.79万 - 项目类别:
Regulation of Salivary Glands-Specific Cystatin S
唾液腺特异性胱抑素 S 的调节
- 批准号:
7455149 - 财政年份:1987
- 资助金额:
$ 22.79万 - 项目类别:
AGE RELATED RESPONSE OF SALIVARY GLANDS TO ISOPROTERENOL
唾液腺对异丙肾上腺素的年龄相关反应
- 批准号:
2129979 - 财政年份:1987
- 资助金额:
$ 22.79万 - 项目类别:
Regulation of Salivary Glands-Specific Cystatin S
唾液腺特异性胱抑素 S 的调节
- 批准号:
6922028 - 财政年份:1987
- 资助金额:
$ 22.79万 - 项目类别:
Regulation of Salivary Glands-Specific Cystatin S
唾液腺特异性胱抑素 S 的调节
- 批准号:
6823909 - 财政年份:1987
- 资助金额:
$ 22.79万 - 项目类别:
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- 批准号:
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