AGE RELATED RESPONSE OF SALIVARY GLANDS TO ISOPROTERENOL

唾液腺对异丙肾上腺素的年龄相关反应

基本信息

项目摘要

Salivary glands produce secretions that bathe and protect the oral cavity. Cystatin S, a naturally occurring cysteine proteinase inhibitor secreted by submandibular glands, is believed to play a major protective role. Until recently, nothing was known about the regulation of cystatin, S gene expression despite the role these proteins play in oral pathophysiology. Our long-range goal is to determine the mechanisms regulating cystatin genes. The goal of the proposed research is to identify the functional regulatory elements of the rat cystatin S gene, highly homologous to human cystatin S genes, and the DNA binding proteins that modulate their function. The Specific aims are to: 1) examine the hormonal, neuronal, and developmental regulation of the rat cystatin S gene, 2) determine the functional cis-acting DNA regulatory elements in the cystatin S gene, and 3) identify, isolate and characterize the trans-acting DNA binding proteins regulating the rat cystatin S gene and the genes encoding them. We will examine cystatin S mRNA levels in the submandibular glands of postnatally developing males, of male and female rats after castration and hormone replacement, and in glands whose innervation was blocked. Transfection of segments of the 5' region of the cystatin S gene in constructs containing the CAT reporter gene into appropriate recipient cells will allow us to determine which of the potential regulatory sequences we have identified are functional. Gel shift and DNase I protection assays will permit us to identify the DNA binding proteins regulating this gene. Purifying these proteins, obtaining their amino acid sequence and synthesizing oligonucleotides corresponding to the amino acid sequence and generating antibodies to these DNA binding proteins will make it possible to clone and characterize the genes encoding these regulatory proteins. Knowledge of how expression of cystatin genes is regulated may lead to clinical treatments for patients who are orally compromised because of periodontal disease, Sjogren's syndrome, and HIV disease.
唾液腺产生的分泌物可以沐浴和保护口腔。

项目成果

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PHYLLIS Ann SHAW其他文献

PHYLLIS Ann SHAW的其他文献

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{{ truncateString('PHYLLIS Ann SHAW', 18)}}的其他基金

Controlled expression of a cystatin C-peptide as therapy for Alzheimer's disease
半胱氨酸蛋白酶抑制剂 C 肽的受控表达作为阿尔茨海默病的治疗方法
  • 批准号:
    8190713
  • 财政年份:
    2011
  • 资助金额:
    $ 22.79万
  • 项目类别:
Controlled expression of a cystatin C-peptide as therapy for Alzheimer's disease
半胱氨酸蛋白酶抑制剂 C 肽的受控表达作为阿尔茨海默病的治疗方法
  • 批准号:
    8323235
  • 财政年份:
    2011
  • 资助金额:
    $ 22.79万
  • 项目类别:
RIBOZYME MEDIATED KNOCKOUT OF THE CYSTATIN S GENE
核酶介导的胱抑素 S 基因敲除
  • 批准号:
    2132565
  • 财政年份:
    1995
  • 资助金额:
    $ 22.79万
  • 项目类别:
RIBOZYME MEDIATED KNOCKOUT OF THE CYSTATIN S GENE
核酶介导的胱抑素 S 基因敲除
  • 批准号:
    2132566
  • 财政年份:
    1995
  • 资助金额:
    $ 22.79万
  • 项目类别:
AGE-RELATED RESPONSE OF SALIVARY GLANDS TO ISOPROTERENOL
唾液腺对异丙肾上腺素的年龄相关反应
  • 批准号:
    3221952
  • 财政年份:
    1987
  • 资助金额:
    $ 22.79万
  • 项目类别:
REGULATION OF CYSTATIN S GENE EXPRESSION
胱抑素S基因表达的调控
  • 批准号:
    6379637
  • 财政年份:
    1987
  • 资助金额:
    $ 22.79万
  • 项目类别:
Regulation of Salivary Glands-Specific Cystatin S
唾液腺特异性胱抑素 S 的调节
  • 批准号:
    7455149
  • 财政年份:
    1987
  • 资助金额:
    $ 22.79万
  • 项目类别:
AGE RELATED RESPONSE OF SALIVARY GLANDS TO ISOPROTERENOL
唾液腺对异丙肾上腺素的年龄相关反应
  • 批准号:
    2129979
  • 财政年份:
    1987
  • 资助金额:
    $ 22.79万
  • 项目类别:
Regulation of Salivary Glands-Specific Cystatin S
唾液腺特异性胱抑素 S 的调节
  • 批准号:
    6922028
  • 财政年份:
    1987
  • 资助金额:
    $ 22.79万
  • 项目类别:
Regulation of Salivary Glands-Specific Cystatin S
唾液腺特异性胱抑素 S 的调节
  • 批准号:
    6823909
  • 财政年份:
    1987
  • 资助金额:
    $ 22.79万
  • 项目类别:

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DNA footprinting of a plant defense gene family; to support visit by A.M. Yorkin, Department of Genetics, St. Petersburg State University, St. Petersburg, Russia
植物防御基因家族的 DNA 足迹;
  • 批准号:
    147394-1992
  • 财政年份:
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  • 资助金额:
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  • 项目类别:
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