LIQUID CHROMATOGRAPHY OF BIOLOGICAL SUBSTANCES

这项建议旨在扩大高性能的范围 液相色谱技术及其在医学和生命科学中的应用 沿着几条同调线的科学。分子间的相互作用 与固定相对生物聚合物的保留有关 将研究表面介导的层析过程 详细地说。本研究的结果是疏水性、静电性 而生物特异性的相互作用有望揭示 生物系统中的相关表面现象并提供一种 用于表征所使用的各种固定相的手段 在实践中。将进行一项重大研究工作，以 开发高效的色谱分离技术 为满足紧急需求而生产的多糖及其结合物 对于用于分析和提纯此类物质的适当工具， 它的生物学意义正日益被人们认识到。 同时，将开发有效的方法来解决 凝集素和低聚糖的分离纯化 与我们对层析的研究结合使用 生物碳水化合物。将这些程序系统化 将允许建立一套用于 从生物基质中分离复杂分子。 在这个项目过程中获得的经验将在广告中使用 关于分居问题的特设合作研究项目 对生命科学和医学的重要性。的另一部分 拟议的研究将集中在蛋白质和蛋白质的快速分析上 用微球吸附剂填充的柱上的核酸 使用适当修改的仪器。这种方法 承诺将分析时间至少减少十分之一。 基于大孔硅质和硅胶的新型固定相 聚合物微球将被开发用于分析 蛋白质、核酸和具有生物重要性的糖； 在这些物质的分析层析中获得的经验 物质将被用来为它们的制备准备吸着剂 分离。非线性力学的理论与实践研究 洗脱色谱有望提供一个框架 制备型色谱仪的分析与设计 进程。

This proposal is aimed at expanding the scope of high performance liquid chromatography and its application to medicine and the life sciences along several congruos lines. The molecular interactions involved in the retention of biopolymers by stationary phases in surface mediated chromatographic processes will be investigated in detail. The results of this study on hydrophobic, electrostatic and biospecific interactions are expected to shed light on the relevant surface phenomena in biological systems and to provide a means for the characterization of various stationary phases used in practice. A major research effort will be undertaken to develop highly efficient chromatographic separation techniques for glycans and their conjugates in order to meet an acute need for suitable tools for analysis and purification of such substances, whose biological significance is being increasingly recognized. Concurrently, efficient methods will be developed for the isolation and purification of lectins and oligosaccharides to be used in conjunction with our research on chromatography of biological carbohydrates. Systematization of these procedures will permit the establishment of a set of unit operations for the isolation of complex molecules from biological matrices. Experience gained in the course of this project will be used in ad hoc collaborative research projects on separation problems of importance to the life sciences and medicine. Another part of the proposed research will focus on the rapid analysis of proteins and nucleic acid on columns packed with micropellicular sorbents and using appropriately modified instrumentation. This approach promises at least a ten-fold reduction in the time of analysis. Novel stationary phases based on macroporous siliceous and polymeric microspheres will be developed for the analysis of proteins, nucleic acids and biologically important sugars; the experience gained in the analytical chromatography of these substances will be used to prepare sorbents for their preparative separation. Studies on the theory and practice of non-linear elution chromatography are expected to provide a framework for the analysis and design of the preparative chromatographic process.