NEUTRAL GLYCOLIPID ANTIGENS

中性糖脂抗原

• 批准号: 2259426
• 负责人: FRANCINE J VRIESENDORP
• 金额: $ 9.02万
• 依托单位: UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-08-01 至 1996-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2259426
• 关键词: Guillain Barre syndrome; complement; complement fixation tests; disease /disorder model; electrophysiology; enzyme linked immunosorbent assay; glycolipids; heterophile antibody; heterophile antigens; histology; laboratory rat; myelinopathy; sciatic nerve; thin layer chromatography

Guillain-Barre' syndrome (GBS) is an immune-mediated, inflammatory, demyelinating polyneuropathy. In serum of GBS patients, complement fixing antibodies against peripheral nerve myelin can be found, which correlate with the clinical course and precede the appearance of activated complement components in CSF, serum and peripheral nerve. A significant portion of this Ab activity in some GBS patients is directed against a neutral glycolipid of peripheral nerve myelin that cross-reacts with Forssman antigen. Forssman is a component of several viruses and bacteria, and heterophile responses accompanying many infections are partly directed against Forssman antigen. In this proposal we want to study the role of antibody and complement in peripheral nerve demyelination in experimental allergic neuritis (EAN), an animal model of GBS. Our specific aims are: 1) To study the development of experimental allergic neuritis in the Lewis rat, following immunization with Forssman antigen. 2) To determine which epitope on Forssman is recognized by serum Ab from Forssman induced EAN animals. 3) To determine if affinity-purified Ab from EAN serum can cause demyelination following intraneural injection in Lewis rat sciatic nerve. 4) To study the effect of complement depletion on blood-nerve barrier integrity in EAN induced by peripheral nerve myelin. Electrophysiologic testing and histologic studies will be done to assess peripheral nerve demyelination in EAN, and after intraneural injection. Immunological techniques to be used include ELISA, C1 fixation-transfer assay and HPTLC.

格林-巴利综合征（GBS）是一种免疫介导的炎症性疾病， 脱髓鞘性多发性神经病 在GBS患者血清中， 可以发现抗周围神经髓鞘的固定抗体， 与临床病程相关，并先于 激活CSF、血清和外周神经中的补体成分。 一 在一些GBS患者中，该Ab活性的显著部分是针对 针对外周神经髓鞘的中性糖脂， 与福斯曼抗原有交叉反应 福斯曼是几个 病毒和细菌，以及伴随许多 感染部分针对福斯曼抗原。 在这 我们想研究抗体和补体在 实验性变态反应性神经炎（EAN）中的外周神经脱髓鞘， GBS的动物模型。 我们的具体目标是：1）研究 在刘易斯大鼠中发生实验性过敏性神经炎， 用Forssman抗原免疫后。2)以确定哪些 Forssman上的表位被来自Forssman诱导的EAN的血清Ab识别 动物3)为了确定从EAN血清中亲和纯化的Ab是否可以 引起刘易斯大鼠坐骨神经内注射后脱髓鞘 神经。4)研究补体耗竭对血神经的影响 周围神经髓鞘诱导的EAN屏障完整性。 将进行电生理测试和组织学研究，以评估 周围神经脱髓鞘在EAN和神经内注射后。 使用的免疫学技术包括ELISA、C1固定-转移 测定和HPTLC。