CRANIOFACIAL CHONDROGENESIS AND RETINOID RECEPTORS

颅面软骨形成和类维生素A受体

基本信息

  • 批准号:
    3087134
  • 负责人:
  • 金额:
    $ 8.1万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    1992
  • 资助国家:
    美国
  • 起止时间:
    1992-07-01 至 1997-06-30
  • 项目状态:
    已结题

项目摘要

The major theme of this proposal is to improve our understanding of how retinoic acid and its receptors contribute to craniofacial development. The first phase of the study will define the temporal and spatial expressions of the specific retinoic acid receptors and cellular retinoic acid binding protein (CRABP) in embryonic mouse craniofacial mesenchyme. This will be done by in situ and Northern blot hybridization. Receptor gene expression from different facial areas will be quantitatively compared using an image analyzer system. Receptor expression will also be studied in embryos exposed to teratogenic doses of exogenous retinoic acid. The second phase of the investigation will analyze the expression of retinoic acid receptors and CRABP in high density micromass cell cultures of mesenchyme taken from different craniofacial areas, with and without exogenous retinoic acid. This study will provide information concerning the role of specific retinoic acid receptors in controlling mesectodermal chondrogenesis. In the studies of both of these phases, special emphasis will be made on observing any differences between first and second brachial arch mesenchyme, because consistent differences in their responses to exogenous retinoids have been found in mice, nonhuman primates, and humans. The third phase probes retinoic acid's role in craniofacial development by blocking expression of retinoic acid receptor genes. Antisense oligonucleotides or retroviruses containing antisense fragments of receptor cDNAs will be used to block receptor expression. The antisense oligonucleotides will be added to the micromass mesectodermal cell cultures. The retroviruses will be injected into the neural fold of mouse embryos, in order to transfect some cells destined to become cranial neural crest. These embryos will then be allowed to grow in whole embryo and micromass cell culture. This series of experiments should provide insight into the developmental regulation of retinoic acid receptors and CRABP during normal craniofacial chondrogenesis and following exogenous application of retinoic acid that is known to induce craniofacial malformations.
这项建议的主要主题是提高我们对如何

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Edward James Lammer其他文献

Edward James Lammer的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Edward James Lammer', 18)}}的其他基金

Chromosomal microdeletions causing heart defects
染色体微缺失导致心脏缺陷
  • 批准号:
    8456072
  • 财政年份:
    2009
  • 资助金额:
    $ 8.1万
  • 项目类别:
Chromosomal microdeletions causing heart defects
染色体微缺失导致心脏缺陷
  • 批准号:
    8297897
  • 财政年份:
    2009
  • 资助金额:
    $ 8.1万
  • 项目类别:
Chromosomal microdeletions causing heart defects
染色体微缺失导致心脏缺陷
  • 批准号:
    7655152
  • 财政年份:
    2009
  • 资助金额:
    $ 8.1万
  • 项目类别:
Chromosomal microdeletions causing heart defects
染色体微缺失导致心脏缺陷
  • 批准号:
    7851378
  • 财政年份:
    2009
  • 资助金额:
    $ 8.1万
  • 项目类别:
Conotruncal defects: genetic and nutritional risk
圆锥干缺陷:遗传和营养风险
  • 批准号:
    7227448
  • 财政年份:
    2006
  • 资助金额:
    $ 8.1万
  • 项目类别:
Conotruncal defects: genetic and nutritional risk
圆锥干缺陷:遗传和营养风险
  • 批准号:
    7091090
  • 财政年份:
    2006
  • 资助金额:
    $ 8.1万
  • 项目类别:
Conotruncal defects: genetic and nutritional risk
圆锥干缺陷:遗传和营养风险
  • 批准号:
    7825376
  • 财政年份:
    2006
  • 资助金额:
    $ 8.1万
  • 项目类别:
Conotruncal defects: genetic and nutritional risk
圆锥干缺陷:遗传和营养风险
  • 批准号:
    7617597
  • 财政年份:
    2006
  • 资助金额:
    $ 8.1万
  • 项目类别:
Conotruncal defects: genetic and nutritional risk
圆锥干缺陷:遗传和营养风险
  • 批准号:
    7416701
  • 财政年份:
    2006
  • 资助金额:
    $ 8.1万
  • 项目类别:
CRANIOFACIAL CHONDROGENESIS AND RETINOID RECEPTORS
颅面软骨形成和类维生素A受体
  • 批准号:
    3087133
  • 财政年份:
    1992
  • 资助金额:
    $ 8.1万
  • 项目类别:
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了