GENE REGULATION IN LENS REGENERATION

成年期晶状体再生是一种独特的现象，仅限于 对一些黑猩猩来说。一旦取出镜片，新的镜片就会重新生成 通过这些细胞的转分化，从虹膜背部色素上皮 细胞到晶状体细胞。这个动作只发生在背部，而不是背部 来自腹侧的虹膜。然而，正如培养眼睛细胞所显示的那样， 视网膜色素上皮、晶状体上皮或视网膜的能力 各种物种，包括老年人，将转化为晶状体 保留体外培养。此外，腹侧虹膜色素上皮是 在体外也能分化。自从细胞培养以来 涉及细胞的解离和黏附，所涉及的分子的调节 在这些进程中应该是一个重要的因素。只有一个人的能力 虹膜背侧在体内再生为晶状体也具有很强的空间性 监管。Homeo盒和包含pax的基因被认为是编码 调控分子参与图案的形成、分化和 轴规范。这些特性使这些基因成为 控制以时空方式调节的事件，镜头 再生就是其中之一。此外，一种含有pax的基因具有 与眼病无虹膜有牵连。我们建议克隆这些 从经历再生的眼睛组织中提取基因并检查它们的 在体内和体外向成骨细胞分化中的表达 镜头。我们打算通过聚合酶链的方法来克隆这些序列 反应(PCR)。表达将通过原位杂交进行研究， Northern分析或聚合酶链反应。这类研究将提供分子工具 研究眼睛的基因调控，帮助理解基本的分子 晶状体再生和转分化的机制 眼睛细胞。

Lens regeneration during adulthood is a unique phenomenon restricted only to some urodeles. Once the lens is removed the new lens is regenerated from the dorsal iris pigmented epithelium by transdifferentiation of these cells to lens cells. This event takes place only from the dorsal but not from the ventral iris. However, as has been shown by culturing eye cells, the ability of pigment epithelium, lens epithelium or retina from a variety of species, including old human, to transdifferentiate to lens in vitro is retained. Furthermore, the ventral iris pigmented epithelium is also capable of differentiation in vitro. Since the culturing of cells involves cell dissociation and adhesion, regulation of molecules involved in these processes should be an important element. The ability of only the dorsal iris to regenerate to lens in vivo also implies strongly spatial regulation. Homeo box- and pax-containing genes, are known to code for regulatory molecules involved in pattern formation, differentiation and axes specification. These properties make these genes good candidates for controlling events that are regulated in a spatio-temporal manner, lens regeneration being one of them. In addition, a pax-containing gene has been implicated in the eye disease aniridia. We propose to clone these genes from the eye tissues that undergo regeneration and examine their expression during the in vivo and in vitro differentiation of cells into lens. We intend to clone these sequences by means of the Polymerase Chain Reaction (PCR). The expression will be studied by in situ hybridization, Northern analysis or PCR. Such studies will provide molecular tools to study gene regulation in the eye and help fathom the basic molecular mechanisms underlying lens regeneration and transdifferentiation of the eye cells.