INTERCELLULAR COMMUNICATION IN THE LENS

研究计划的长远目标是取得详细的 3透镜光纤间隙连接的功能特性信息 连接蛋白结构与功能的关系。 如果我们想了解这些过程的详细信息， 了解不同连接蛋白的功能特性如何有助于 器官内环境的稳定以及白内障的发生 有五个具体目标： 1.进一步表征3个透镜间隙的功能特性 连接蛋白在爪蟾卵母细胞对中表达。 2.为了表征单个非连接cx46诱导的 通道在孤立的卵母细胞。 3.确定cx46和cx50的羧基端结构域的作用， 渠道功能。 4.为了确定其他蛋白质结构域在通道门控中的作用， 渗透 5.为了表征大鼠cx46和大鼠cx50的功能特性， 使用双膜片钳的连接蛋白转染的哺乳动物细胞系 法

The long term objectives of the research proposal are to obtain detailed information about the functional properties of 3 lens fiber gap junctional proteins and the relationship between connexin structure and function. Detailed knowledge about these processes is important if we are to understand how the functional properties of different connexins contribute to organ homeostasis and how cataracts arise. There are 5 specific aims: 1. To characterize further the functional properties of 3 lens gap junctional proteins expressed in Xenopus oocyte pairs. 2. To characterize the behavior of single, nonjunctional cx46-induced channels in solitary oocytes. 3. To determine the role of the carboxyl terminal domain of cx46 and cx50 in channel function. 4. To determine the role of other protein domains in channel gating and permeation. 5. To characterize the functional properties of rat cx46 and rat cx50 in connexin-transfected mammalian cell lines using the dual patch clamp technique.