GENOME SEQUENCING CENTER

拟建的基因组测序中心将扩大测序能力 中国国际研究院正在进行的多重测序项目。一个额外的焦点 将是新技术和软件的发展，以期 显著提高吞吐量。40名个人将参与到 两个项目和两个核心。以下是他们的目标摘要。 项目1：自动多重测序。总体目标是 五个序列中的2.4、4、6.4、11.3和20Mb的邻接区 连续几年的项目。增加的吞吐量将是 通过实验室和计算机程序的自动化来完成。这个 麻风菌和结核分枝杆菌基因组将首先完成。这个 测序目标将转移到人类染色体10q24-q26和它的 小鼠共线区域。总体而言，将有24人参与这一活动 项目。预计第5年的成本约为每个基地25美分。 项目2：红外探测和自动化。该项目将重点放在 自动化高通量杂交和红外荧光 侦测。红外荧光的使用将显著增加 吞吐量，简化质量控制，并通过消除 使用放射性化学品和胶片。通过集成自动 杂交和检测，所有人工过程中的 多重测序的杂交/检测部分将是 被淘汰了。在五年的时间里，六台机器实现了自动化 将构建杂交和检测，使原始数据在 每年超过500 Mb的速率。总体而言，将有7个人 参与这个项目。 信息学核心。总体来说，5年的目标是发展和 实施能够维持处理速度超过 1.5Mb的原始序列数据和大约100kb的完成序列 一天。需要解决的四个方面是：改善基地呼叫 例程，改进序列组装，自动编辑重叠群，以及 开发工具以促进序列分析。附加系统 管理和计算任务包括网络和硬件升级， 数据备份、开发项目管理工具和编程 支持机器人自动化。总体而言，这一核心将涉及8 个人。

The proposed Genome Sequencing Center will expand the sequencing capacity of the ongoing multiplex sequencing project at CRI. An additional focus will be the development of new technology and software in order to significantly increase throughput. Forty individuals will be involved in two projects and two cores. A summary of their goals follows. Project 1: Automated Multiplex Sequencing. The overall goal is to sequence 2.4, 4, 6.4, 11.3 and 20 Mb contiguous regions in the five consecutive years of the project. The increased throughput will be accomplished by automating both laboratory and computer procedures. The M. leprae and M. tuberculosis genomes will be completed first. The sequencing target will then shift to human chromosome 10q24-q26 and its mouse syntenic regions. Overall, 24 individuals will participate in this project. The projected cost in Year 5 is approximately 25 cents per base. Project 2: Infrared Detection and Automation. This project will focus on automated high throughput hybridization and infrared fluorescence detection. The use of infrared fluorescence will significantly increase throughput, simplify quality control, and reduce costs by eliminating the use of radioactive chemicals and films. By integrating automatic hybridization and detection, all manual processes in the hybridization/detection component of multiplex sequencing will be eliminated. Over the course of five years, six machines for automated hybridization and detection will be built, enabling raw data generation at a rate of more than 500 Mb per year. Overall, 7 individuals will participate in this project. Informatics Core. The overall, 5-year objective is to develop and implement software that is capable of sustaining a processing rate of over 1.5 Mb of raw sequence data and approximately 100 kb of finished sequence per day. The four areas to be addressed are: improving base calling routines, improving sequence assembly, automating contig editing, and developing tools to facilitate sequence analysis. Additional system administration and computing tasks include network and hardware upgrades, data backups, development of project management tools, and programming support for robotics automation. Overall, this core will involve 8 individuals.