Electrical Detection of Small Molecule Binding to Biological Receptors using Organic Thin Film Transistors : A new approach for label free assays

使用有机薄膜晶体管对小分子与生物受体结合的电检测：一种无标记测定的新方法

• 批准号: 133593
• 金额: $ 8.84万
• 依托单位: NEUDRIVE LIMITED
• 依托单位国家: 英国
• 项目类别: Feasibility Studies
• 财政年份: 2018
• 资助国家: 英国
• 起止时间: 2018 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=133593
• 关键词: Electrical; Detection; Small; Molecule; Binding

Despite continual advances in our knowledge of human biology, with the attendant opportunity for therapeutic interventions in newly discovered biological pathways, the pharmaceutical industry faces a number of significant challenges. Foremost amongst these is a need to improve the efficiency of drug discovery, reducing the time to market for new medicines and lowering attrition in the later stages of drug development. Optimisation of methods for the measurement of the interaction between drug 'candidate' compounds and their desired or undesired biological targets have a critical role to play in this. Label free analysis approaches remain enduringly popular, as they can provide a direct measure of these interactions; at the current time these approaches involve optical detection using precision instrumentation. Drawbacks of the current state of the art include high instrumentation and operating costs and limited sample throughput (the number of 'candidate' compounds that can be measured in a day). Non-optical detection approaches can also be considered. This project aims to introduce a disruptive technology approach for label free analysis, based on the use of organic thin film transistor (OTFT) biosensors, where the detection is electrical in nature. OTFT offers a route to low cost fabrication of biosensor arrays, inspired by low temperature, additive fabrication methods originally designed for the consumer electronics industries. Electrical detection using OTFT promises to disrupt the label free analysis market by dispensing with optical instrumentation and will enable massive parallelisation compared to current technologies, resulting in lowered costs and a step change in productivity for end users within the pharmaceutical industry. The project will demonstrate the feasibility of using OTFT biosensors for label free analysis, using exemplifying systems selected for their relevance to the drug discovery process.

尽管人类生物学知识不断进步，伴随着新发现的生物学途径的治疗干预机会，制药行业面临着许多重大挑战。其中最重要的是需要提高药物发现的效率，减少新药上市的时间，并减少药物开发后期的损耗。用于测量药物“候选”化合物与其期望或不期望的生物靶标之间的相互作用的方法的优化在这方面起着关键作用。无标记分析方法仍然非常受欢迎，因为它们可以提供这些相互作用的直接测量;目前这些方法涉及使用精密仪器的光学检测。现有技术的缺点包括高仪器和操作成本以及有限的样品通量（一天中可以测量的“候选”化合物的数量）。也可以考虑非光学检测方法。该项目旨在引入一种基于有机薄膜晶体管（OTFT）生物传感器的无标签分析的颠覆性技术方法，其中检测本质上是电学的。OTFT提供了一种低成本制造生物传感器阵列的途径，其灵感来自最初为消费电子行业设计的低温增材制造方法。使用OTFT的电检测有望通过免除光学仪器来扰乱无标签分析市场，并将实现与当前技术相比的大规模并行化，从而降低成本并为制药行业的最终用户带来生产力的阶跃变化。该项目将证明使用OTFT生物传感器进行无标签分析的可行性，使用与药物发现过程相关的示例系统。