DEVELOPMENTAL VIROLOGY RESEARCH

艾滋病是一种病毒性疾病，临床进展与 增加了病毒负担。对体内HIV-1水平的准确评估将是 对监测治疗效果至关重要。我们的实验室是第一个 发展可测定感染性滴度的定量培养方法 在患者的血浆和外周血单核细胞中检测到HIV-1。 此外，我们还利用这些终点稀释法来 证明体内是否存在抗病毒药物的有效性 探员们。近两年来，HIV-1 DNA在临床上的定量 我们实验室也已经实现了用聚合酶链式反应方法检测样品。这 定量聚合酶链式反应技术已经能够检测到 在我们遇到的患者中HIV-1的载量。我们现在提议提炼 这些定量技术使我们能够准确和定量地 检测传染性HIV-1及其核酸在多个临床中的应用 标本。

AIDS is a viral disease, and clinical progression is associated with increased viral burden. Accurate assessment of HIV-1 level in vivo will be critical for monitoring treatment efficacy. Our laboratory was the first to develop quantitative cultures which can determine the infectious titers of HIV-1 in the plasma and peripheral blood mononuclear cells of patients. Furthermore, we have utilized these end-point-dilution assays to demonstrate the presence or absence of in vivo efficacy of antiviral agents. In the past two years, quantitation of HIV-1 DNA in clinical samples by PCR methods has also been achieved in our laboratory. This quantitative PCR technique has permitted the detection of fluctuations in HIV-1 load in the patients encountered by us. We now propose to refine these quantitative techniques so that we can accurately and quantitatively measure infectious HIV-1 and its nucleic acids in a number of clinical specimens.